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The map label for this gene is yiaE [C]

Identifier: 118581356

GI number: 118581356

Start: 3221697

End: 3222665

Strand: Direct

Name: yiaE [C]

Synonym: Ppro_2951

Alternate gene names: 118581356

Gene position: 3221697-3222665 (Clockwise)

Preceding gene: 118581355

Following gene: 118581357

Centisome position: 80.38

GC content: 61.82

Gene sequence:

>969_bases
ATGAATATTGTCGTGCTGGACGGATACACCCTGAACCCGGGAGACTTAAGCTGGGACGACCTGAAGGAACTGGGTGACAC
GGTCGTCTACGAGCGAACCCCGGCAGACAGGACGGTGGAACGTATCGGCAACGCTGAAATCGTCATCACCAACAAGACAG
TCATCGACCGGGATGTGCTGGACTCCTGTCCGTCACTGCGCTATGTGGGGGTCCTGGCAACGGGGTACAACGTGGTTGAC
GTGGAGACAGCACGTGAGCGGGGGATAACCGTCACCAACGTGCCCAGCTACGGGACAAGCGCCGTCGGCCAGTTCGCCAT
CGCCCTGCTTCTGGAGATCTGCCATCACATAGGCCTGCATGGCCGGAGCGTCAGGGAGGGGGAGTGGGCGGAGAGCGCCG
ACTTCTGCTACTGGAAACAACCGCTGATCGAACTGGACGGCAAGACCATGGGCATCATCGGTTTCGGGCGCATTGGCCGC
AAAACCGGGCAGATCGCCCAGGCCCTGGGAATGAGGGTCCTGGCTGCGGGCAGCAGGAGAGTCCCGGAACGGGAAAGCGA
GACCATGCAGTACGTGGAGCTGGACGAACTGCTGGAAAAGTCGGACGTCATCTCCCTGCACTGCCCCCTGTCGGAAAAGA
CCCGTGGGATCATCAACAGGGACAGCATCGCCAGGATGAAGGACGGGGTGATCATCATCAACACCTCCCGCGGCCCGTTG
ATCGTCGAAGAGGACCTGGCCCAGGCGCTCAGCTCCGGCAAGGTCTACGCCGCCGGCCTGGACGTCGTCTCCGTCGAGCC
GGTGCAAAGCGACAATCCGCTTTTGAGCGCCAGGAACTGCATCATCACCCCGCACATCGCCTGGGCGCCGAAGGAGGCGC
GGGAACGACTGCTGGAGATCGCCGTGGGCAACGTCAGGAGCTTCTGTGCCGGATCGCCGGTGAATGTGGTCAACAGAGAG
GAGAAGTGA

Upstream 100 bases:

>100_bases
GTCGCTGATGCACATACTGAAAATTAGCGCTTCGGGCCCCCATATCGTGACACGGCTTTGATGCCGCACCTTGTACCCGA
TACGGAGAGGAGTCTTGATC

Downstream 100 bases:

>100_bases
GCATACAATGCGTGGCCGCTGCCATGAGCGGCACCATGGCGGTTCTACCGAAAACAGGTCACGACTACCAGGAGGGAGCT
ATCTCATGAACGAAAAAACA

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: hydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNIVVLDGYTLNPGDLSWDDLKELGDTVVYERTPADRTVERIGNAEIVITNKTVIDRDVLDSCPSLRYVGVLATGYNVVD
VETARERGITVTNVPSYGTSAVGQFAIALLLEICHHIGLHGRSVREGEWAESADFCYWKQPLIELDGKTMGIIGFGRIGR
KTGQIAQALGMRVLAAGSRRVPERESETMQYVELDELLEKSDVISLHCPLSEKTRGIINRDSIARMKDGVIIINTSRGPL
IVEEDLAQALSSGKVYAAGLDVVSVEPVQSDNPLLSARNCIITPHIAWAPKEARERLLEIAVGNVRSFCAGSPVNVVNRE
EK

Sequences:

>Translated_322_residues
MNIVVLDGYTLNPGDLSWDDLKELGDTVVYERTPADRTVERIGNAEIVITNKTVIDRDVLDSCPSLRYVGVLATGYNVVD
VETARERGITVTNVPSYGTSAVGQFAIALLLEICHHIGLHGRSVREGEWAESADFCYWKQPLIELDGKTMGIIGFGRIGR
KTGQIAQALGMRVLAAGSRRVPERESETMQYVELDELLEKSDVISLHCPLSEKTRGIINRDSIARMKDGVIIINTSRGPL
IVEEDLAQALSSGKVYAAGLDVVSVEPVQSDNPLLSARNCIITPHIAWAPKEARERLLEIAVGNVRSFCAGSPVNVVNRE
EK
>Mature_322_residues
MNIVVLDGYTLNPGDLSWDDLKELGDTVVYERTPADRTVERIGNAEIVITNKTVIDRDVLDSCPSLRYVGVLATGYNVVD
VETARERGITVTNVPSYGTSAVGQFAIALLLEICHHIGLHGRSVREGEWAESADFCYWKQPLIELDGKTMGIIGFGRIGR
KTGQIAQALGMRVLAAGSRRVPERESETMQYVELDELLEKSDVISLHCPLSEKTRGIINRDSIARMKDGVIIINTSRGPL
IVEEDLAQALSSGKVYAAGLDVVSVEPVQSDNPLLSARNCIITPHIAWAPKEARERLLEIAVGNVRSFCAGSPVNVVNRE
EK

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=267, Percent_Identity=34.0823970037453, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI23308577, Length=276, Percent_Identity=32.9710144927536, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI145580578, Length=316, Percent_Identity=31.9620253164557, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557499, Length=316, Percent_Identity=31.9620253164557, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580575, Length=316, Percent_Identity=31.9620253164557, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI4557497, Length=311, Percent_Identity=28.9389067524116, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI61743967, Length=311, Percent_Identity=28.9389067524116, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI87082289, Length=275, Percent_Identity=32.7272727272727, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789279, Length=289, Percent_Identity=31.8339100346021, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787645, Length=259, Percent_Identity=31.2741312741313, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87081824, Length=155, Percent_Identity=35.4838709677419, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=281, Percent_Identity=29.1814946619217, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=243, Percent_Identity=34.5679012345679, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=311, Percent_Identity=31.8327974276527, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=325, Percent_Identity=31.0769230769231, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322116, Length=345, Percent_Identity=30.1449275362319, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324964, Length=302, Percent_Identity=29.8013245033113, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324980, Length=183, Percent_Identity=30.0546448087432, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=147, Percent_Identity=34.6938775510204, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=171, Percent_Identity=28.0701754385965, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28574286, Length=293, Percent_Identity=29.3515358361775, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28571528, Length=260, Percent_Identity=33.4615384615385, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24585514, Length=303, Percent_Identity=27.7227722772277, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28574282, Length=303, Percent_Identity=27.7227722772277, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28574284, Length=303, Percent_Identity=27.7227722772277, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45551003, Length=303, Percent_Identity=27.7227722772277, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45552429, Length=305, Percent_Identity=28.1967213114754, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646446, Length=282, Percent_Identity=31.9148936170213, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646448, Length=282, Percent_Identity=31.9148936170213, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646452, Length=282, Percent_Identity=31.9148936170213, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646450, Length=282, Percent_Identity=31.9148936170213, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI62472511, Length=282, Percent_Identity=31.9148936170213, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585516, Length=296, Percent_Identity=25.6756756756757, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19921140, Length=288, Percent_Identity=29.1666666666667, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.29

Molecular weight: Translated: 35252; Mature: 35252

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVVLDGYTLNPGDLSWDDLKELGDTVVYERTPADRTVERIGNAEIVITNKTVIDRDVL
CEEEEECCEEECCCCCCHHHHHHHCCEEEEECCCCHHHHHHCCCEEEEEECCCEECHHHH
DSCPSLRYVGVLATGYNVVDVETARERGITVTNVPSYGTSAVGQFAIALLLEICHHIGLH
HCCCCCEEEEEEECCCEEEEEEHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GRSVREGEWAESADFCYWKQPLIELDGKTMGIIGFGRIGRKTGQIAQALGMRVLAAGSRR
CCCCCCCCCCCCCCCEEECCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCC
VPERESETMQYVELDELLEKSDVISLHCPLSEKTRGIINRDSIARMKDGVIIINTSRGPL
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCHHHHHHHCCEEEEECCCCCE
IVEEDLAQALSSGKVYAAGLDVVSVEPVQSDNPLLSARNCIITPHIAWAPKEARERLLEI
EEHHHHHHHHHCCCEEEECCCEEEECCCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHH
AVGNVRSFCAGSPVNVVNREEK
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNIVVLDGYTLNPGDLSWDDLKELGDTVVYERTPADRTVERIGNAEIVITNKTVIDRDVL
CEEEEECCEEECCCCCCHHHHHHHCCEEEEECCCCHHHHHHCCCEEEEEECCCEECHHHH
DSCPSLRYVGVLATGYNVVDVETARERGITVTNVPSYGTSAVGQFAIALLLEICHHIGLH
HCCCCCEEEEEEECCCEEEEEEHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GRSVREGEWAESADFCYWKQPLIELDGKTMGIIGFGRIGRKTGQIAQALGMRVLAAGSRR
CCCCCCCCCCCCCCCEEECCCCEEECCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCC
VPERESETMQYVELDELLEKSDVISLHCPLSEKTRGIINRDSIARMKDGVIIINTSRGPL
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCHHHHHHHCCEEEEECCCCCE
IVEEDLAQALSSGKVYAAGLDVVSVEPVQSDNPLLSARNCIITPHIAWAPKEARERLLEI
EEHHHHHHHHHCCCEEEECCCEEEECCCCCCCCEEECCCEEEECCCCCCCHHHHHHHHHH
AVGNVRSFCAGSPVNVVNREEK
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-glycerate; NAD+

Specific reaction: (R)-glycerate + NAD+ = hydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]