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The map label for this gene is fabI [H]

Identifier: 118581299

GI number: 118581299

Start: 3165718

End: 3166488

Strand: Direct

Name: fabI [H]

Synonym: Ppro_2894

Alternate gene names: 118581299

Gene position: 3165718-3166488 (Clockwise)

Preceding gene: 118581298

Following gene: 118581300

Centisome position: 78.98

GC content: 60.7

Gene sequence:

>771_bases
ATGTCACTATTACAGGGCAAAAAAGCGCTCATCTTCGGCATTGCCAACGACAAGAGCATCGCCTGGGCCATTGCGCAGGC
GATGCACCGACAGGGAGCCGAACTGGCCGTCACCTATGCGGGAGAAGCACTGGAAAAGCGGGTCCGCCCCCTGGCGGAGT
CACTGAACGCCACAGCAATCCTCCCCTGCAACGTCACCTGCGACGAGGACATAGCATCCGTAATAAAGCACCTCTCAACC
ATCTGGGACGGCATCGACATCATCGTCCATGCCATAGCCTTCGCCAACAAGGAAGAATTGAAGGGGACCATCCTCAACAC
CACCAGGGAAGGGTTCGCTACCGCCATGGACATCAGCGCCTACTCCTTCATCGCCATACTGAAGGAGGCACACGCCATGA
TGCAGGGGCGAAACGCAGCAGCACTGACCCTCACCTACCACGGCGCCAGGAAGGTCTTCCCCAATTACAATGTCATGGGC
GTGGCCAAGGCGGCCCTGGAGGCATCGGTGCACTACCTGTCCGAGGCGCTCGGCCCCGAGGGTATCCGGGTCAACGCCAT
CTCAGCCGGCCCGATCCGCACCCTGGCATCGGCAGGCGTCAGCGGTTTCGCGCATATCCTCAACCAGGTTGAGGGTAAGG
CGCCCCTGCGCCGCAACACCACCCAGGAAGACGTGGCCGGTTCGGCGGTCTACCTGTGCAGCGATCTGGCCAATGCCGTC
ACCGGCGAGGTTCACTACGTCGACTGCGGTTACAATGTGATCGGACTGTAG

Upstream 100 bases:

>100_bases
CCCGGCAGCAGCGACAGGCTATCGACCTGACTCCCGCTGCCTTCAGTTATCACATGCATTCCCATGACCACCTTATCTCA
AACCGGAGGATAGAACACCT

Downstream 100 bases:

>100_bases
CCTCCCCCAGCACGACACCCGAATAAAGAAGGCCCTGGATGCCGGAATGGTCCAGGGCCTCTACATTGCCACCTCACGGC
CTGGAACGCCGCGCGCTTGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSLLQGKKALIFGIANDKSIAWAIAQAMHRQGAELAVTYAGEALEKRVRPLAESLNATAILPCNVTCDEDIASVIKHLST
IWDGIDIIVHAIAFANKEELKGTILNTTREGFATAMDISAYSFIAILKEAHAMMQGRNAAALTLTYHGARKVFPNYNVMG
VAKAALEASVHYLSEALGPEGIRVNAISAGPIRTLASAGVSGFAHILNQVEGKAPLRRNTTQEDVAGSAVYLCSDLANAV
TGEVHYVDCGYNVIGL

Sequences:

>Translated_256_residues
MSLLQGKKALIFGIANDKSIAWAIAQAMHRQGAELAVTYAGEALEKRVRPLAESLNATAILPCNVTCDEDIASVIKHLST
IWDGIDIIVHAIAFANKEELKGTILNTTREGFATAMDISAYSFIAILKEAHAMMQGRNAAALTLTYHGARKVFPNYNVMG
VAKAALEASVHYLSEALGPEGIRVNAISAGPIRTLASAGVSGFAHILNQVEGKAPLRRNTTQEDVAGSAVYLCSDLANAV
TGEVHYVDCGYNVIGL
>Mature_255_residues
SLLQGKKALIFGIANDKSIAWAIAQAMHRQGAELAVTYAGEALEKRVRPLAESLNATAILPCNVTCDEDIASVIKHLSTI
WDGIDIIVHAIAFANKEELKGTILNTTREGFATAMDISAYSFIAILKEAHAMMQGRNAAALTLTYHGARKVFPNYNVMGV
AKAALEASVHYLSEALGPEGIRVNAISAGPIRTLASAGVSGFAHILNQVEGKAPLRRNTTQEDVAGSAVYLCSDLANAVT
GEVHYVDCGYNVIGL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=49.2125984251969, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27179; Mature: 27048

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLQGKKALIFGIANDKSIAWAIAQAMHRQGAELAVTYAGEALEKRVRPLAESLNATAI
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
LPCNVTCDEDIASVIKHLSTIWDGIDIIVHAIAFANKEELKGTILNTTREGFATAMDISA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHCCHHHHHHHHHHHH
YSFIAILKEAHAMMQGRNAAALTLTYHGARKVFPNYNVMGVAKAALEASVHYLSEALGPE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLASAGVSGFAHILNQVEGKAPLRRNTTQEDVAGSAVYLCSDLANAV
CEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
TGEVHYVDCGYNVIGL
CCCEEEEECCCEEECC
>Mature Secondary Structure 
SLLQGKKALIFGIANDKSIAWAIAQAMHRQGAELAVTYAGEALEKRVRPLAESLNATAI
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEE
LPCNVTCDEDIASVIKHLSTIWDGIDIIVHAIAFANKEELKGTILNTTREGFATAMDISA
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHCCHHHHHHHHHHHH
YSFIAILKEAHAMMQGRNAAALTLTYHGARKVFPNYNVMGVAKAALEASVHYLSEALGPE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GIRVNAISAGPIRTLASAGVSGFAHILNQVEGKAPLRRNTTQEDVAGSAVYLCSDLANAV
CEEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
TGEVHYVDCGYNVIGL
CCCEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]