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The map label for this gene is nadE [H]

Identifier: 118581273

GI number: 118581273

Start: 3137881

End: 3138879

Strand: Reverse

Name: nadE [H]

Synonym: Ppro_2868

Alternate gene names: 118581273

Gene position: 3138879-3137881 (Counterclockwise)

Preceding gene: 118581274

Following gene: 118581272

Centisome position: 78.32

GC content: 49.85

Gene sequence:

>999_bases
ATGAATGGAACGGTGCACCATTTGCGTGAGGCATTGCGCCTGAACCCGGACAATGAAGCGTCGAGGATCAGTCGCGAAAT
TCGTGAGATGATGCTGGGCCAGATAAAAAGGCGCGGCCTGGTCGTTGGGCTCTCCGGCGGAATCGACAGCAGCGTCACAG
CGGCGCTTGCCGTGAAGGCCCTGGGGCAGGACAGGGTTCTGGGGTTACTGATGCCTGAGCGAAATTCGTCCGGAGATTCC
TTGCATCTTGGCAAGATGGTGGCTGGCTATCTCGGCATAGAGACAGTTACGGAAGAGATTACCAACATACTTGAGGTAGT
TGGGTTCTATCAAAAGTACGATTCAGCTGTTTGCCGGGTAATACCCGAATACGGCAGGGGGTGGAAATCCAAAATAGTAA
TATCAAACGCTCTGAAGCAACATTCCTATGCATCATTTTATATTGTTGCCCAGCCCAAAGAAGGGGCGGTTATCAAGAAA
CGCCTGTCCCTTGAGCCTTATCTGGAAATTGTCGCCGCGACAAACTTTAAACAACGCATACGAAAAATGCTGGAGTACTA
CCATGCCGATCGTCTGAACTACGCCGTTGCGGGCACACCGAACCGTCTGGAGTATGATCAGGGATTTTTTGTAAAACTTG
GTGACGGAGCTGCTGACATAAAACCGATTGCGCACCTGTACAAGTCACAGGTTTACCAGCTTGCAGAATACTTCGGCATA
CCGGAAGAGATCAGGCAGCGGAAACCGACCACGGATACCTATTCTCTGGAGCAGGGGCAGGATGAATTCTTTTTTACGCT
CCCCTATGATCAGATGGATCTCTGTCTATTTGGCAAGAACAATTCCGTTCCAGCCGGGGACATTGCCAACGCACTCGGCA
TGCCAGAGCAACACATTGAAATTATTTATAAAGATATCGACACAAAACGCGCCACCACGCGTTACCTCCATCTTTCAGCG
CTAGTGATGGAAAATGGTATACTTTCATACACAGCTTGA

Upstream 100 bases:

>100_bases
AACGATGAATCCGGGCCGCATAGGCCGAAATTGTGATTGATGCGGGTTTAAAAGCCATTGTTAGTGCAGTTCCCCAAACA
CGGAACGGAGAGGAGGACCG

Downstream 100 bases:

>100_bases
CTACCTGATACGACCTGCAGGGAGGCGACAGTCCAATGCGCGAAAGCGAAAAAATCAGAGCATTCATAATCGATAATTTT
CTTTTTGGGGAAGACCGTGG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNGTVHHLREALRLNPDNEASRISREIREMMLGQIKRRGLVVGLSGGIDSSVTAALAVKALGQDRVLGLLMPERNSSGDS
LHLGKMVAGYLGIETVTEEITNILEVVGFYQKYDSAVCRVIPEYGRGWKSKIVISNALKQHSYASFYIVAQPKEGAVIKK
RLSLEPYLEIVAATNFKQRIRKMLEYYHADRLNYAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYFGI
PEEIRQRKPTTDTYSLEQGQDEFFFTLPYDQMDLCLFGKNNSVPAGDIANALGMPEQHIEIIYKDIDTKRATTRYLHLSA
LVMENGILSYTA

Sequences:

>Translated_332_residues
MNGTVHHLREALRLNPDNEASRISREIREMMLGQIKRRGLVVGLSGGIDSSVTAALAVKALGQDRVLGLLMPERNSSGDS
LHLGKMVAGYLGIETVTEEITNILEVVGFYQKYDSAVCRVIPEYGRGWKSKIVISNALKQHSYASFYIVAQPKEGAVIKK
RLSLEPYLEIVAATNFKQRIRKMLEYYHADRLNYAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYFGI
PEEIRQRKPTTDTYSLEQGQDEFFFTLPYDQMDLCLFGKNNSVPAGDIANALGMPEQHIEIIYKDIDTKRATTRYLHLSA
LVMENGILSYTA
>Mature_332_residues
MNGTVHHLREALRLNPDNEASRISREIREMMLGQIKRRGLVVGLSGGIDSSVTAALAVKALGQDRVLGLLMPERNSSGDS
LHLGKMVAGYLGIETVTEEITNILEVVGFYQKYDSAVCRVIPEYGRGWKSKIVISNALKQHSYASFYIVAQPKEGAVIKK
RLSLEPYLEIVAATNFKQRIRKMLEYYHADRLNYAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYFGI
PEEIRQRKPTTDTYSLEQGQDEFFFTLPYDQMDLCLFGKNNSVPAGDIANALGMPEQHIEIIYKDIDTKRATTRYLHLSA
LVMENGILSYTA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 37292; Mature: 37292

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGTVHHLREALRLNPDNEASRISREIREMMLGQIKRRGLVVGLSGGIDSSVTAALAVKA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
LGQDRVLGLLMPERNSSGDSLHLGKMVAGYLGIETVTEEITNILEVVGFYQKYDSAVCRV
CCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPEYGRGWKSKIVISNALKQHSYASFYIVAQPKEGAVIKKRLSLEPYLEIVAATNFKQRI
HHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHCCHHHHH
RKMLEYYHADRLNYAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYFGI
HHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PEEIRQRKPTTDTYSLEQGQDEFFFTLPYDQMDLCLFGKNNSVPAGDIANALGMPEQHIE
CHHHHCCCCCCCCEECCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHH
IIYKDIDTKRATTRYLHLSALVMENGILSYTA
HHHHHCCCHHHHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MNGTVHHLREALRLNPDNEASRISREIREMMLGQIKRRGLVVGLSGGIDSSVTAALAVKA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
LGQDRVLGLLMPERNSSGDSLHLGKMVAGYLGIETVTEEITNILEVVGFYQKYDSAVCRV
CCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPEYGRGWKSKIVISNALKQHSYASFYIVAQPKEGAVIKKRLSLEPYLEIVAATNFKQRI
HHHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHHCCHHHHH
RKMLEYYHADRLNYAVAGTPNRLEYDQGFFVKLGDGAADIKPIAHLYKSQVYQLAEYFGI
HHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
PEEIRQRKPTTDTYSLEQGQDEFFFTLPYDQMDLCLFGKNNSVPAGDIANALGMPEQHIE
CHHHHCCCCCCCCEECCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHH
IIYKDIDTKRATTRYLHLSALVMENGILSYTA
HHHHHCCCHHHHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]