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The map label for this gene is mycA [H]

Identifier: 118581177

GI number: 118581177

Start: 3030831

End: 3032585

Strand: Reverse

Name: mycA [H]

Synonym: Ppro_2770

Alternate gene names: 118581177

Gene position: 3032585-3030831 (Counterclockwise)

Preceding gene: 118581178

Following gene: 118581176

Centisome position: 75.66

GC content: 64.22

Gene sequence:

>1755_bases
ATGCATACACTCAAAAAGACACCCACTAATAACAGCATCGCCCTGCGCCGCGGCGACTTCAACAGCCTGCCCGAAGCTCT
CGACTATGCCGCCGAAGGCAGGACCGGGTTCAATTTCTACTCCGGCAAGGGAAGCCTGGCAGTCACGCTTCCCTATTCCC
AGATGCGTGACAGCGCCCGCTCCCTTGCCCGCAAGCTCGTGGGGCTCGGCCTGTCGCGGGGGGATCGCATGGCCCTGGTC
GCCGAGACCGATGCTGATTTCGTGCGTTTCTTCTTCGCCTGCCAGTATGCCGGGTTGGTTCCCGTGCCTCTGCCCGCCGC
GGTTCATCTGGGGGGGCGCCAGGCCATTATCGATAACCTGCGAAAACTGGTGGAGTCCTGCGGAGCTAGGGCCGCCATGT
CTTCCCGCTCCTTCTTTGCCTTCCTGTCCAAGGCTGCCGAGGGGCTCGACCTGAAACACGTGGGGATACCCGACTGCTTC
GACGCGCTTCCTGAGGCCGACCAGATGTTGCAGGCGCCGGACCCCGAGGATGTTGCCTATCTGCAGTACACTTCCGGCAG
CACCCGCTTTCCCCGCGGCGTGGTGATCACCCAGAAGTCGATCATGGCCAACCTGGCCTGCATCATCAGCCATGGCCTAG
CCGTCCAGCCGGGCGACCGGGCCACCTCGTGGCTCCCCTTCTACCACGATATGGGACTGGTCGGTTTCATGCTTGCCCCC
ATGGCCTGCCAGCTCTCCATCGATTACCTGAGCACCCGCGATTTCGCCATGCGGCCGCGCCTCTGGCCGGCGCTGATCTC
CAAAAACCGGGCCACCATCTCCTACAGTCCAACCTTTGGCTACGCGCTGTGCGCGCGGCGTCTCCGGCCGGCCGAGATCG
AACAGTACGATCTCAGTTCCTGGCGCATCGCCGGAACCGGGGCCGAGCCGATACGGGCGGATATCCTGGAGAACTTCGGC
AAACTGCTGGCGCCGGCCGGTTTCAGTTCCACAGCCTATGTGGCCTCCTACGGCATGGCCGAGTGTTCCCTGGCAATCAG
TTTCGCCCCCTTGGGCAGGGGGCTGGAGATCGACCGGATCGATGCCGAAGCGCTCACCAACAGCGGACGCGCCATTCCCC
TTGAAGAGGGGGCCAGCGGGGCAGAGGTGAAGGAACTCGTCCTGTGCGGTTCGGTGATCCCCGGCCATGAGATCGAGATC
CGCGACGAGCATGGCATGCCGCTCCCCGAACGGTGCTGCGGTGTGTTGCACGTGCGCGGTCCCAGCGTCATGCGCGAATA
CCTGGGAGCCCCGGAGACCACCCGGGAGGTGCTTTGCCCGGATGGGTGGCTCAATACGGGCGACCTGGCCTATCTGGCCG
GGGGGCAGGTGGTCATCACCGGCCGCATGAAGGACCTGATCATCATCAATGGCCGCAATATCTGGCCCCAGGATATCGAA
TTCGTCGTCGAACAGTTGCCGGAAATCCGTTCCCGGGACGCTGCCGCCTTTTCCATTCCCGGCCAGGGAGGTGAGGAACA
GGTGGTGCTGGTGGTGCAGTGCCGCCATTTTGACGAGACCGAGCGTTGCCGGCTGGTGGAGCTGCTCCGAGGCCGGGTCC
GTGAGGAGTTCGGCGTCGACTGCCTGGTCGAGCTGGTTGCCCCCCATACCCTGCCCCAGACATCGTCGGGGAAGATCTCG
CGCTCCATGGCCAAGGCGGATTACCTGCGGCGCATCGGCGACGCCTCCTCCCCAGCCCGTGCGGGACAAGACTGA

Upstream 100 bases:

>100_bases
CTGTGCCAATGACGTGGGCGATGAAACCCGCCAGGCCATCAGGCAGCTCATACTCTCACACTGGGACTAACATCACTGCT
CCCCCAAAGTATCCGATACT

Downstream 100 bases:

>100_bases
GCCATGGGGGAGCCGGAGGGGGCAGTTCCTTTATCCCCTGGGCTGACCGTAGCCCTTACCGGAGGGACCGGGTTCGTCGG
CGGTGCCATCATCCGCCGTC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MHTLKKTPTNNSIALRRGDFNSLPEALDYAAEGRTGFNFYSGKGSLAVTLPYSQMRDSARSLARKLVGLGLSRGDRMALV
AETDADFVRFFFACQYAGLVPVPLPAAVHLGGRQAIIDNLRKLVESCGARAAMSSRSFFAFLSKAAEGLDLKHVGIPDCF
DALPEADQMLQAPDPEDVAYLQYTSGSTRFPRGVVITQKSIMANLACIISHGLAVQPGDRATSWLPFYHDMGLVGFMLAP
MACQLSIDYLSTRDFAMRPRLWPALISKNRATISYSPTFGYALCARRLRPAEIEQYDLSSWRIAGTGAEPIRADILENFG
KLLAPAGFSSTAYVASYGMAECSLAISFAPLGRGLEIDRIDAEALTNSGRAIPLEEGASGAEVKELVLCGSVIPGHEIEI
RDEHGMPLPERCCGVLHVRGPSVMREYLGAPETTREVLCPDGWLNTGDLAYLAGGQVVITGRMKDLIIINGRNIWPQDIE
FVVEQLPEIRSRDAAAFSIPGQGGEEQVVLVVQCRHFDETERCRLVELLRGRVREEFGVDCLVELVAPHTLPQTSSGKIS
RSMAKADYLRRIGDASSPARAGQD

Sequences:

>Translated_584_residues
MHTLKKTPTNNSIALRRGDFNSLPEALDYAAEGRTGFNFYSGKGSLAVTLPYSQMRDSARSLARKLVGLGLSRGDRMALV
AETDADFVRFFFACQYAGLVPVPLPAAVHLGGRQAIIDNLRKLVESCGARAAMSSRSFFAFLSKAAEGLDLKHVGIPDCF
DALPEADQMLQAPDPEDVAYLQYTSGSTRFPRGVVITQKSIMANLACIISHGLAVQPGDRATSWLPFYHDMGLVGFMLAP
MACQLSIDYLSTRDFAMRPRLWPALISKNRATISYSPTFGYALCARRLRPAEIEQYDLSSWRIAGTGAEPIRADILENFG
KLLAPAGFSSTAYVASYGMAECSLAISFAPLGRGLEIDRIDAEALTNSGRAIPLEEGASGAEVKELVLCGSVIPGHEIEI
RDEHGMPLPERCCGVLHVRGPSVMREYLGAPETTREVLCPDGWLNTGDLAYLAGGQVVITGRMKDLIIINGRNIWPQDIE
FVVEQLPEIRSRDAAAFSIPGQGGEEQVVLVVQCRHFDETERCRLVELLRGRVREEFGVDCLVELVAPHTLPQTSSGKIS
RSMAKADYLRRIGDASSPARAGQD
>Mature_584_residues
MHTLKKTPTNNSIALRRGDFNSLPEALDYAAEGRTGFNFYSGKGSLAVTLPYSQMRDSARSLARKLVGLGLSRGDRMALV
AETDADFVRFFFACQYAGLVPVPLPAAVHLGGRQAIIDNLRKLVESCGARAAMSSRSFFAFLSKAAEGLDLKHVGIPDCF
DALPEADQMLQAPDPEDVAYLQYTSGSTRFPRGVVITQKSIMANLACIISHGLAVQPGDRATSWLPFYHDMGLVGFMLAP
MACQLSIDYLSTRDFAMRPRLWPALISKNRATISYSPTFGYALCARRLRPAEIEQYDLSSWRIAGTGAEPIRADILENFG
KLLAPAGFSSTAYVASYGMAECSLAISFAPLGRGLEIDRIDAEALTNSGRAIPLEEGASGAEVKELVLCGSVIPGHEIEI
RDEHGMPLPERCCGVLHVRGPSVMREYLGAPETTREVLCPDGWLNTGDLAYLAGGQVVITGRMKDLIIINGRNIWPQDIE
FVVEQLPEIRSRDAAAFSIPGQGGEEQVVLVVQCRHFDETERCRLVELLRGRVREEFGVDCLVELVAPHTLPQTSSGKIS
RSMAKADYLRRIGDASSPARAGQD

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=517, Percent_Identity=24.5647969052224, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761343, Length=517, Percent_Identity=24.5647969052224, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI44888818, Length=542, Percent_Identity=24.169741697417, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI55749758, Length=577, Percent_Identity=23.9168110918544, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=26.9439421338156, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI145693145, Length=558, Percent_Identity=24.7311827956989, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=575, Percent_Identity=25.2173913043478, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=558, Percent_Identity=22.9390681003584, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=521, Percent_Identity=24.3761996161228, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=415, Percent_Identity=23.6144578313253, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=180, Percent_Identity=32.7777777777778, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=180, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI18859661, Length=553, Percent_Identity=25.497287522604, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076582, Length=239, Percent_Identity=28.0334728033473, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653035, Length=149, Percent_Identity=31.5436241610738, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63608; Mature: 63608

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTLKKTPTNNSIALRRGDFNSLPEALDYAAEGRTGFNFYSGKGSLAVTLPYSQMRDSAR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCEEEEECHHHHHHHHH
SLARKLVGLGLSRGDRMALVAETDADFVRFFFACQYAGLVPVPLPAAVHLGGRQAIIDNL
HHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHEECCCHHHHHHHH
RKLVESCGARAAMSSRSFFAFLSKAAEGLDLKHVGIPDCFDALPEADQMLQAPDPEDVAY
HHHHHHCCCHHHHCCHHHHHHHHHHHCCCCEECCCCCHHHHHCCCHHHHHCCCCCCCEEE
LQYTSGSTRFPRGVVITQKSIMANLACIISHGLAVQPGDRATSWLPFYHDMGLVGFMLAP
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHC
MACQLSIDYLSTRDFAMRPRLWPALISKNRATISYSPTFGYALCARRLRPAEIEQYDLSS
HHHEEEEHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCC
WRIAGTGAEPIRADILENFGKLLAPAGFSSTAYVASYGMAECSLAISFAPLGRGLEIDRI
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCEEECC
DAEALTNSGRAIPLEEGASGAEVKELVLCGSVIPGHEIEIRDEHGMPLPERCCGVLHVRG
CHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHCCEEEECC
PSVMREYLGAPETTREVLCPDGWLNTGDLAYLAGGQVVITGRMKDLIIINGRNIWPQDIE
HHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECCEEEEEECCCEEEEECCCCCCHHHHH
FVVEQLPEIRSRDAAAFSIPGQGGEEQVVLVVQCRHFDETERCRLVELLRGRVREEFGVD
HHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHH
CLVELVAPHTLPQTSSGKISRSMAKADYLRRIGDASSPARAGQD
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHTLKKTPTNNSIALRRGDFNSLPEALDYAAEGRTGFNFYSGKGSLAVTLPYSQMRDSAR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCEEEEECHHHHHHHHH
SLARKLVGLGLSRGDRMALVAETDADFVRFFFACQYAGLVPVPLPAAVHLGGRQAIIDNL
HHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHEECCCHHHHHHHH
RKLVESCGARAAMSSRSFFAFLSKAAEGLDLKHVGIPDCFDALPEADQMLQAPDPEDVAY
HHHHHHCCCHHHHCCHHHHHHHHHHHCCCCEECCCCCHHHHHCCCHHHHHCCCCCCCEEE
LQYTSGSTRFPRGVVITQKSIMANLACIISHGLAVQPGDRATSWLPFYHDMGLVGFMLAP
EEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHC
MACQLSIDYLSTRDFAMRPRLWPALISKNRATISYSPTFGYALCARRLRPAEIEQYDLSS
HHHEEEEHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCC
WRIAGTGAEPIRADILENFGKLLAPAGFSSTAYVASYGMAECSLAISFAPLGRGLEIDRI
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCEEECC
DAEALTNSGRAIPLEEGASGAEVKELVLCGSVIPGHEIEIRDEHGMPLPERCCGVLHVRG
CHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHCCEEEECC
PSVMREYLGAPETTREVLCPDGWLNTGDLAYLAGGQVVITGRMKDLIIINGRNIWPQDIE
HHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEECCEEEEEECCCEEEEECCCCCCHHHHH
FVVEQLPEIRSRDAAAFSIPGQGGEEQVVLVVQCRHFDETERCRLVELLRGRVREEFGVD
HHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHH
CLVELVAPHTLPQTSSGKISRSMAKADYLRRIGDASSPARAGQD
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]