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The map label for this gene is dfrA [H]

Identifier: 118581176

GI number: 118581176

Start: 3029871

End: 3030827

Strand: Reverse

Name: dfrA [H]

Synonym: Ppro_2769

Alternate gene names: 118581176

Gene position: 3030827-3029871 (Counterclockwise)

Preceding gene: 118581177

Following gene: 118581175

Centisome position: 75.62

GC content: 68.13

Gene sequence:

>957_bases
ATGGGGGAGCCGGAGGGGGCAGTTCCTTTATCCCCTGGGCTGACCGTAGCCCTTACCGGAGGGACCGGGTTCGTCGGCGG
TGCCATCATCCGCCGTCTGCTGGAGGATCGGGTGCGCGTCAGGGCGCTGGTGCGGCCGGCTTCCCTGGCATCCTGTTTCG
AAGCAAAAGGGTTGACCTGGATCCATGGCGAACTGGGGGACCGGGAGAGCCTGCGACGGCTGGTGGAGGGTGCCTCGGCC
GTTATCCACTGCGCCGGTTCCGTTCGCGGCGCCTGCCCGGCCGACTTTGAGCCGGCCAATGTCAGCGGAGTGGAGCGCAT
CGTGGCGGCAGCCCGTGAGTCGGCCGGTCATCCGCGGTTGCTCCTGTTCTCATCCCTGGCCGCCAGGTCGCCCGAACTGT
CCGACTATGCCGCCAGCAAGCGGCGGGGCGAGGATGCCCTGCGCAGCGCTGCCCAGGGGCTTGACTGGACGATCCTGCGA
CCGCCGGCGGTGTACGGTCCCGGAGACCGGGAGATGCTCCCCCTGCTGCAGTGGATGCGCCGGGGAATCCTGTTCGTTCC
GGGGAGCGGTGCGGGGCGCCTCTCGCTGATCTACGTCAGCGACCTTGCCGAAGCCGTCCTGTGCTGGCTCATGAACGGCA
GCGGCCTGAACGGTTGCTTTGAACCGCATGACGGCAAGGCAGGCGGCTACAGCTGGGAGGAGATCAGGGGGGTCGCCAGG
GAGCTTTACCGGCGCTCCGTCCTCCGCGTCGACATTCCGCGCCCGCTTCTGGAGCTGGCGGCGCGCCTGAACATCCCTGC
ATCACGGTTCTTCGGCTATCGGCCGATGCTCACGCCGGGCAAGGTCAGGGAGCTCTGCCACCAGGACTGGGTCTGCCATG
ATGAGGATTTTCGCCGGGCAGTCAACTGGAGCCCCCGGGTGGGGCTGGCCGAGGGATTGCGGCGCACCCTGGGATGA

Upstream 100 bases:

>100_bases
GACATCGTCGGGGAAGATCTCGCGCTCCATGGCCAAGGCGGATTACCTGCGGCGCATCGGCGACGCCTCCTCCCCAGCCC
GTGCGGGACAAGACTGAGCC

Downstream 100 bases:

>100_bases
TGGAACGGTTCAAACCCCGAATCCGGCGGGCGAAGCAGGCCAGGAAATTCACGACAAGGAAAGCATGACGATGGCCACAG
CAGACTATACGGAAATCTAT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MGEPEGAVPLSPGLTVALTGGTGFVGGAIIRRLLEDRVRVRALVRPASLASCFEAKGLTWIHGELGDRESLRRLVEGASA
VIHCAGSVRGACPADFEPANVSGVERIVAAARESAGHPRLLLFSSLAARSPELSDYAASKRRGEDALRSAAQGLDWTILR
PPAVYGPGDREMLPLLQWMRRGILFVPGSGAGRLSLIYVSDLAEAVLCWLMNGSGLNGCFEPHDGKAGGYSWEEIRGVAR
ELYRRSVLRVDIPRPLLELAARLNIPASRFFGYRPMLTPGKVRELCHQDWVCHDEDFRRAVNWSPRVGLAEGLRRTLG

Sequences:

>Translated_318_residues
MGEPEGAVPLSPGLTVALTGGTGFVGGAIIRRLLEDRVRVRALVRPASLASCFEAKGLTWIHGELGDRESLRRLVEGASA
VIHCAGSVRGACPADFEPANVSGVERIVAAARESAGHPRLLLFSSLAARSPELSDYAASKRRGEDALRSAAQGLDWTILR
PPAVYGPGDREMLPLLQWMRRGILFVPGSGAGRLSLIYVSDLAEAVLCWLMNGSGLNGCFEPHDGKAGGYSWEEIRGVAR
ELYRRSVLRVDIPRPLLELAARLNIPASRFFGYRPMLTPGKVRELCHQDWVCHDEDFRRAVNWSPRVGLAEGLRRTLG
>Mature_317_residues
GEPEGAVPLSPGLTVALTGGTGFVGGAIIRRLLEDRVRVRALVRPASLASCFEAKGLTWIHGELGDRESLRRLVEGASAV
IHCAGSVRGACPADFEPANVSGVERIVAAARESAGHPRLLLFSSLAARSPELSDYAASKRRGEDALRSAAQGLDWTILRP
PAVYGPGDREMLPLLQWMRRGILFVPGSGAGRLSLIYVSDLAEAVLCWLMNGSGLNGCFEPHDGKAGGYSWEEIRGVARE
LYRRSVLRVDIPRPLLELAARLNIPASRFFGYRPMLTPGKVRELCHQDWVCHDEDFRRAVNWSPRVGLAEGLRRTLG

Specific function: Unknown

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 34568; Mature: 34437

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPEGAVPLSPGLTVALTGGTGFVGGAIIRRLLEDRVRVRALVRPASLASCFEAKGLTW
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEE
IHGELGDRESLRRLVEGASAVIHCAGSVRGACPADFEPANVSGVERIVAAARESAGHPRL
EECCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLFSSLAARSPELSDYAASKRRGEDALRSAAQGLDWTILRPPAVYGPGDREMLPLLQWMR
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHH
RGILFVPGSGAGRLSLIYVSDLAEAVLCWLMNGSGLNGCFEPHDGKAGGYSWEEIRGVAR
CCEEEECCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
ELYRRSVLRVDIPRPLLELAARLNIPASRFFGYRPMLTPGKVRELCHQDWVCHDEDFRRA
HHHHHHHHEECCCHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VNWSPRVGLAEGLRRTLG
CCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GEPEGAVPLSPGLTVALTGGTGFVGGAIIRRLLEDRVRVRALVRPASLASCFEAKGLTW
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEE
IHGELGDRESLRRLVEGASAVIHCAGSVRGACPADFEPANVSGVERIVAAARESAGHPRL
EECCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLFSSLAARSPELSDYAASKRRGEDALRSAAQGLDWTILRPPAVYGPGDREMLPLLQWMR
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHH
RGILFVPGSGAGRLSLIYVSDLAEAVLCWLMNGSGLNGCFEPHDGKAGGYSWEEIRGVAR
CCEEEECCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
ELYRRSVLRVDIPRPLLELAARLNIPASRFFGYRPMLTPGKVRELCHQDWVCHDEDFRRA
HHHHHHHHEECCCHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VNWSPRVGLAEGLRRTLG
CCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]