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The map label for this gene is hupR1 [H]

Identifier: 118581132

GI number: 118581132

Start: 2973331

End: 2974188

Strand: Reverse

Name: hupR1 [H]

Synonym: Ppro_2723

Alternate gene names: 118581132

Gene position: 2974188-2973331 (Counterclockwise)

Preceding gene: 118581133

Following gene: 118581131

Centisome position: 74.21

GC content: 58.16

Gene sequence:

>858_bases
ATGGATCCCGAACCAATCCGCATACTTCTGGTCGATGACGAACGGAACGTGCTCCGGTCACTGGAACGGCTCTTTCTGGA
TGAAGAGTACGAGATCTTCACCGCCGGTTCCGGGGGGGATGGGTTGGAGATTCTGGAACAGCACGGTCCGTTCCGGTTGG
TTATTTCGGACTACCGCATGTCCGGCATGCACGGCGTCGAGTTCTTGAGCGAGGTCTGTCGTCGTTGGCCCGATACGGAA
CGGATGATCCTCTCCGGTTATGCCGACCTGGCCGCCATCGTGGCTTCCATCAACCTGGGGCGGATCTACAAGTTCATCGC
CAAGCCGTGGAACGATGATGACCTGCTCTGTACCGTCCGCGAGGCGCTGGAACGCTACGAATTGCGGGCCAGAAACCGTC
AACTTGTGACGGATCTGATGGCTGCCAACGAAGAATTGCGGAGAATCAACGACCATCTGAATGACATGGTGGACGAGCGT
GTCAAGGAAGTGCTGCTGAAGAGCCGCGCCCTTCACGGTTTTCAGGGCGTGGTGGACGCGCTGCCGATAGGGGTCGTGGG
GGCGGACGGATACGGCATGGTCGTCCAGTGCAACGCCCTTGGAGCCGAGATGCTGGGTGTCGATCGAGAAGATCTAATCG
GTCGTGACCTGGCTACCATGTTCCCCCGGCATCTGGTCCAATCCATTGTGCAGTTGGATTACAATTCGGGGTGCACATGT
TCGGTGGAGTTGCCCAGCGGCGCCTGCCGAGCGTTCCTGACGCGGATTGAACAGGGGGATCAGCGGGCGATCGTAATTGC
GCTGATGAAGGATACTATGACGTCCGGAGGAGCCGGATATGGCTATGGATATGGATGA

Upstream 100 bases:

>100_bases
TCCGGGCTGTCCTGGACGGTGAGGCTGGGGGAGATGTCCCGGTATAAACGCATGGGATGGAACTCTCGGCGCATTGCCGA
TGGACGGGGAGAGAACAAAG

Downstream 100 bases:

>100_bases
AACGCATCAGGCCGGGCAGGGGCCGATTTCGGTCCTGCTGGTCGATGACGAAGAGTACATACTGACGTCGCTTCGGCGTC
TTTTGATGGATGAGAAGGAT

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MDPEPIRILLVDDERNVLRSLERLFLDEEYEIFTAGSGGDGLEILEQHGPFRLVISDYRMSGMHGVEFLSEVCRRWPDTE
RMILSGYADLAAIVASINLGRIYKFIAKPWNDDDLLCTVREALERYELRARNRQLVTDLMAANEELRRINDHLNDMVDER
VKEVLLKSRALHGFQGVVDALPIGVVGADGYGMVVQCNALGAEMLGVDREDLIGRDLATMFPRHLVQSIVQLDYNSGCTC
SVELPSGACRAFLTRIEQGDQRAIVIALMKDTMTSGGAGYGYGYG

Sequences:

>Translated_285_residues
MDPEPIRILLVDDERNVLRSLERLFLDEEYEIFTAGSGGDGLEILEQHGPFRLVISDYRMSGMHGVEFLSEVCRRWPDTE
RMILSGYADLAAIVASINLGRIYKFIAKPWNDDDLLCTVREALERYELRARNRQLVTDLMAANEELRRINDHLNDMVDER
VKEVLLKSRALHGFQGVVDALPIGVVGADGYGMVVQCNALGAEMLGVDREDLIGRDLATMFPRHLVQSIVQLDYNSGCTC
SVELPSGACRAFLTRIEQGDQRAIVIALMKDTMTSGGAGYGYGYG
>Mature_285_residues
MDPEPIRILLVDDERNVLRSLERLFLDEEYEIFTAGSGGDGLEILEQHGPFRLVISDYRMSGMHGVEFLSEVCRRWPDTE
RMILSGYADLAAIVASINLGRIYKFIAKPWNDDDLLCTVREALERYELRARNRQLVTDLMAANEELRRINDHLNDMVDER
VKEVLLKSRALHGFQGVVDALPIGVVGADGYGMVVQCNALGAEMLGVDREDLIGRDLATMFPRHLVQSIVQLDYNSGCTC
SVELPSGACRAFLTRIEQGDQRAIVIALMKDTMTSGGAGYGYGYG

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=125, Percent_Identity=33.6, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 31837; Mature: 31837

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPEPIRILLVDDERNVLRSLERLFLDEEYEIFTAGSGGDGLEILEQHGPFRLVISDYRM
CCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCEEEEEECCCC
SGMHGVEFLSEVCRRWPDTERMILSGYADLAAIVASINLGRIYKFIAKPWNDDDLLCTVR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHH
EALERYELRARNRQLVTDLMAANEELRRINDHLNDMVDERVKEVLLKSRALHGFQGVVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPIGVVGADGYGMVVQCNALGAEMLGVDREDLIGRDLATMFPRHLVQSIVQLDYNSGCTC
CCCCEECCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
SVELPSGACRAFLTRIEQGDQRAIVIALMKDTMTSGGAGYGYGYG
EEECCCHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDPEPIRILLVDDERNVLRSLERLFLDEEYEIFTAGSGGDGLEILEQHGPFRLVISDYRM
CCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCEEEEEECCCC
SGMHGVEFLSEVCRRWPDTERMILSGYADLAAIVASINLGRIYKFIAKPWNDDDLLCTVR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHH
EALERYELRARNRQLVTDLMAANEELRRINDHLNDMVDERVKEVLLKSRALHGFQGVVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPIGVVGADGYGMVVQCNALGAEMLGVDREDLIGRDLATMFPRHLVQSIVQLDYNSGCTC
CCCCEECCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
SVELPSGACRAFLTRIEQGDQRAIVIALMKDTMTSGGAGYGYGYG
EEECCCHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]