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The map label for this gene is crp [H]

Identifier: 118581012

GI number: 118581012

Start: 2823715

End: 2824404

Strand: Reverse

Name: crp [H]

Synonym: Ppro_2600

Alternate gene names: 118581012

Gene position: 2824404-2823715 (Counterclockwise)

Preceding gene: 118581013

Following gene: 118581011

Centisome position: 70.47

GC content: 62.9

Gene sequence:

>690_bases
ATGGAACTGGTGGAACAGCTGAAACAGTCCCTGCTCTTCTCCGGTCTGTCCGGTGATGACCTGGCCGACCTGGCCACCAT
CACCGTGCGACGCAGGTTCGCCAAAGGGGAGTCCCTGTTCTGCGAGGGAGAGCAGGCCACAGGCTTCTATCTGCTGGTGG
CCGGGAGCATCAAGCTCTGCCGCGTCTCCCCGGATGGGCGCGAAAAGGTGCTCCACTTCGTCAAACCGCGGGAGACCTTT
GCCGAGGCGGCTTTTTTCGGCGACGGCAGATACCCGGCCGAGGCCCGCGCCATGGAGAGTGGCGAGGCGCTTTTCCTCCC
CCGTGAGGGGTTCATGGAGCTGATGAGTCGCAAGCCGCAGTTATCCCTCAACCTGGTGGTCTCCCTCTCCCTGATGCTGC
GCCAGTTCGTGCGGCAGATCGAGGAGCTCACCTTCGCCGATGTCACCTCCCGGCTGGCCTCGTTCCTGGTGCGGCGGGCA
GAAGAAAAGGCGGCCAGCTATGGCGGCATCACCTACATCGACCTGGGGATCAAAAAGGGGGAGCTGGCTTCCCGGCTGGG
CACGGCCAGCGAGACCATCTCCCGAACCCTGCGCAAGCTTAAGGATGAGGGGATCATCGAGGTGCAGGGAAGCCGGGTGC
TGATCCACCGCATGGACAAACTGCAGAAACTGTGCGAACGACACGAGTAG

Upstream 100 bases:

>100_bases
GCCTCGGCGCCGGGAGATGTGCTGGCCAGGCAGTTCGGCCTCACTCCGCCCCAGGTGGCCGAGAAGATCGCCGTCTTCCT
GAAGAGGTAAGCGTCCGTAC

Downstream 100 bases:

>100_bases
GTGCGGCACCGTGTGGCCGCCCAAGATGTGGCCACACCGGTTGCCGCTCGGGTTTCGGGGATAAGGAGAATTCCAGATGC
TGATCGTCATGAATCATACG

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MELVEQLKQSLLFSGLSGDDLADLATITVRRRFAKGESLFCEGEQATGFYLLVAGSIKLCRVSPDGREKVLHFVKPRETF
AEAAFFGDGRYPAEARAMESGEALFLPREGFMELMSRKPQLSLNLVVSLSLMLRQFVRQIEELTFADVTSRLASFLVRRA
EEKAASYGGITYIDLGIKKGELASRLGTASETISRTLRKLKDEGIIEVQGSRVLIHRMDKLQKLCERHE

Sequences:

>Translated_229_residues
MELVEQLKQSLLFSGLSGDDLADLATITVRRRFAKGESLFCEGEQATGFYLLVAGSIKLCRVSPDGREKVLHFVKPRETF
AEAAFFGDGRYPAEARAMESGEALFLPREGFMELMSRKPQLSLNLVVSLSLMLRQFVRQIEELTFADVTSRLASFLVRRA
EEKAASYGGITYIDLGIKKGELASRLGTASETISRTLRKLKDEGIIEVQGSRVLIHRMDKLQKLCERHE
>Mature_229_residues
MELVEQLKQSLLFSGLSGDDLADLATITVRRRFAKGESLFCEGEQATGFYLLVAGSIKLCRVSPDGREKVLHFVKPRETF
AEAAFFGDGRYPAEARAMESGEALFLPREGFMELMSRKPQLSLNLVVSLSLMLRQFVRQIEELTFADVTSRLASFLVRRA
EEKAASYGGITYIDLGIKKGELASRLGTASETISRTLRKLKDEGIIEVQGSRVLIHRMDKLQKLCERHE

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=190, Percent_Identity=24.2105263157895, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787595, Length=210, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25666; Mature: 25666

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVEQLKQSLLFSGLSGDDLADLATITVRRRFAKGESLFCEGEQATGFYLLVAGSIKLC
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCEEEE
RVSPDGREKVLHFVKPRETFAEAAFFGDGRYPAEARAMESGEALFLPREGFMELMSRKPQ
EECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECHHHHHHHHCCCCC
LSLNLVVSLSLMLRQFVRQIEELTFADVTSRLASFLVRRAEEKAASYGGITYIDLGIKKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
ELASRLGTASETISRTLRKLKDEGIIEVQGSRVLIHRMDKLQKLCERHE
HHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELVEQLKQSLLFSGLSGDDLADLATITVRRRFAKGESLFCEGEQATGFYLLVAGSIKLC
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCEEEE
RVSPDGREKVLHFVKPRETFAEAAFFGDGRYPAEARAMESGEALFLPREGFMELMSRKPQ
EECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECHHHHHHHHCCCCC
LSLNLVVSLSLMLRQFVRQIEELTFADVTSRLASFLVRRAEEKAASYGGITYIDLGIKKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCH
ELASRLGTASETISRTLRKLKDEGIIEVQGSRVLIHRMDKLQKLCERHE
HHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]