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The map label for this gene is thiC2 [H]

Identifier: 118581008

GI number: 118581008

Start: 2818635

End: 2819933

Strand: Direct

Name: thiC2 [H]

Synonym: Ppro_2596

Alternate gene names: 118581008

Gene position: 2818635-2819933 (Clockwise)

Preceding gene: 118581007

Following gene: 118581009

Centisome position: 70.33

GC content: 63.82

Gene sequence:

>1299_bases
ATGACCCAGCTTGAATACGCCCGCAGGGGTGTCATAACCGAAAAGATGAAGATAGCGGCCCAATCCGAGGGAGTAGCCCC
CGAATTCATCCGCGACGGCATCGCCGCCGGCACCATCGTCATCTGCCACAACATCAAACATCTGAACGGCACGCCACTGC
CGGTGGGAACCGGCCTGCGCACCAAGATCAACGCCAATATCGGCTCGTCATCGGATGACACCGACATGCAGAAGGAGTTG
GAAAAGGCACGGGTAGCCGTTAAATACGGAGCCGACGCCATCATGGACCTGTCCACCGGCGGGCCGGTGGACGAGATCCG
ACGCGCCGTCGTGGCCGAGACCAACGCCTGCATCGGCAGCGTGCCGCTCTACCAGGCCGCCCTGGATGCGGTGCGCGTCA
AGAAGAAGGCCATCGTCGACATGACCGTGGACGACATCTTCGCCGGCATCATCAAGCATGCCGAGGATGGCGTGGACTTC
ATCACCGTGCACTGCGGCGTGACCCGCTCCACCGTGGAGAGAATGAAGAACGAGGGACGCATCATGGACGTGGTCTCCCG
TGGCGGCGCCTTCACCATCGAGTGGATGGCCTACAACAACCAGGACAACCCGCTGTTTGAACATTTCGACAAACTGCTGG
AGATCACCAAAGAGTACGACATGACCCTCTCCCTGGGAGACGGCTTCCGCCCCGGCTGCCTGGCCGATGCCACCGACCGG
GCCCAGATCCACGAACTGATCCTGCTGGGAGAACTGACCCAGCGCGCCCAGGCCGCCGGCGTGCAGGTCATGATCGAGGG
GCCTGGCCATGTGCCGCTGCAACAGATCCAGGCCAACATACTGCTGCAGAAGCGTCTCTGTCACGGCGCCCCCTTCTACG
TGCTGGGACCGCTGGTGACCGACATCGCCCCCGGTTACGACCACATCACCTGCGCCATCGGCGGCGCCATAGCCGCCGCG
GCCGGAGCCGACTTCCTCTGCTATGTCACCCCCAGCGAGCACCTGCGCCTGCCTGACGTTCAGGACGTGCGTGACGGCGT
GATCGCTTCCCGCATCGCCGCCCACGCCGGCGACATTGCCAAGGGGGTCAAGGGGGCCATGAACAAGGACATCCAGATGG
CCAAGTGCAGGAAGAAGCTTGACTGGGAGGGGCAGTACTCCCTGGCCCTGGATCCGGAGCGCGCCCGCACCCTGCGGGAG
AGCTCGCCGGTCTCCGATCACGGTGCCTGCACCATGTGCGGCGAGTTCTGCGCCTACAAGGTCATGGACGACGCCATGGC
CCGCCAGGCAGCCAGCTAA

Upstream 100 bases:

>100_bases
GAGCGCATCAGGGCTCTTTCGTCGTAATTACCGACGAAATGGAGCGCGAACACAGACAGCTTCCAACTACCAAATCTAGG
TTTTATACAACGGAGATCCC

Downstream 100 bases:

>100_bases
GGGACAACGGCAGTTTTCCACGCGGCAGGCTTCAGGTTCACGGGGGAGACTCCTGGTACACACCAGGAGTCTCCCCCATT
GTACATCCCCCTCCCCTCAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2 [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTQLEYARRGVITEKMKIAAQSEGVAPEFIRDGIAAGTIVICHNIKHLNGTPLPVGTGLRTKINANIGSSSDDTDMQKEL
EKARVAVKYGADAIMDLSTGGPVDEIRRAVVAETNACIGSVPLYQAALDAVRVKKKAIVDMTVDDIFAGIIKHAEDGVDF
ITVHCGVTRSTVERMKNEGRIMDVVSRGGAFTIEWMAYNNQDNPLFEHFDKLLEITKEYDMTLSLGDGFRPGCLADATDR
AQIHELILLGELTQRAQAAGVQVMIEGPGHVPLQQIQANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIAAA
AGADFLCYVTPSEHLRLPDVQDVRDGVIASRIAAHAGDIAKGVKGAMNKDIQMAKCRKKLDWEGQYSLALDPERARTLRE
SSPVSDHGACTMCGEFCAYKVMDDAMARQAAS

Sequences:

>Translated_432_residues
MTQLEYARRGVITEKMKIAAQSEGVAPEFIRDGIAAGTIVICHNIKHLNGTPLPVGTGLRTKINANIGSSSDDTDMQKEL
EKARVAVKYGADAIMDLSTGGPVDEIRRAVVAETNACIGSVPLYQAALDAVRVKKKAIVDMTVDDIFAGIIKHAEDGVDF
ITVHCGVTRSTVERMKNEGRIMDVVSRGGAFTIEWMAYNNQDNPLFEHFDKLLEITKEYDMTLSLGDGFRPGCLADATDR
AQIHELILLGELTQRAQAAGVQVMIEGPGHVPLQQIQANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIAAA
AGADFLCYVTPSEHLRLPDVQDVRDGVIASRIAAHAGDIAKGVKGAMNKDIQMAKCRKKLDWEGQYSLALDPERARTLRE
SSPVSDHGACTMCGEFCAYKVMDDAMARQAAS
>Mature_431_residues
TQLEYARRGVITEKMKIAAQSEGVAPEFIRDGIAAGTIVICHNIKHLNGTPLPVGTGLRTKINANIGSSSDDTDMQKELE
KARVAVKYGADAIMDLSTGGPVDEIRRAVVAETNACIGSVPLYQAALDAVRVKKKAIVDMTVDDIFAGIIKHAEDGVDFI
TVHCGVTRSTVERMKNEGRIMDVVSRGGAFTIEWMAYNNQDNPLFEHFDKLLEITKEYDMTLSLGDGFRPGCLADATDRA
QIHELILLGELTQRAQAAGVQVMIEGPGHVPLQQIQANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITCAIGGAIAAAA
GADFLCYVTPSEHLRLPDVQDVRDGVIASRIAAHAGDIAKGVKGAMNKDIQMAKCRKKLDWEGQYSLALDPERARTLRES
SPVSDHGACTMCGEFCAYKVMDDAMARQAAS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=456, Percent_Identity=41.6666666666667, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 46634; Mature: 46503

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEYARRGVITEKMKIAAQSEGVAPEFIRDGIAAGTIVICHNIKHLNGTPLPVGTGLR
CCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
TKINANIGSSSDDTDMQKELEKARVAVKYGADAIMDLSTGGPVDEIRRAVVAETNACIGS
EEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
VPLYQAALDAVRVKKKAIVDMTVDDIFAGIIKHAEDGVDFITVHCGVTRSTVERMKNEGR
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCC
IMDVVSRGGAFTIEWMAYNNQDNPLFEHFDKLLEITKEYDMTLSLGDGFRPGCLADATDR
EEEHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCH
AQIHELILLGELTQRAQAAGVQVMIEGPGHVPLQQIQANILLQKRLCHGAPFYVLGPLVT
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITCAIGGAIAAAAGADFLCYVTPSEHLRLPDVQDVRDGVIASRIAAHAGDIA
HCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KGVKGAMNKDIQMAKCRKKLDWEGQYSLALDPERARTLRESSPVSDHGACTMCGEFCAYK
HHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VMDDAMARQAAS
HHHHHHHHHCCC
>Mature Secondary Structure 
TQLEYARRGVITEKMKIAAQSEGVAPEFIRDGIAAGTIVICHNIKHLNGTPLPVGTGLR
CCHHHHHCCCHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCC
TKINANIGSSSDDTDMQKELEKARVAVKYGADAIMDLSTGGPVDEIRRAVVAETNACIGS
EEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
VPLYQAALDAVRVKKKAIVDMTVDDIFAGIIKHAEDGVDFITVHCGVTRSTVERMKNEGR
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCC
IMDVVSRGGAFTIEWMAYNNQDNPLFEHFDKLLEITKEYDMTLSLGDGFRPGCLADATDR
EEEHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCH
AQIHELILLGELTQRAQAAGVQVMIEGPGHVPLQQIQANILLQKRLCHGAPFYVLGPLVT
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITCAIGGAIAAAAGADFLCYVTPSEHLRLPDVQDVRDGVIASRIAAHAGDIA
HCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KGVKGAMNKDIQMAKCRKKLDWEGQYSLALDPERARTLRESSPVSDHGACTMCGEFCAYK
HHHHHHHCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VMDDAMARQAAS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA