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The map label for this gene is thiDE [H]

Identifier: 118581007

GI number: 118581007

Start: 2817006

End: 2818490

Strand: Direct

Name: thiDE [H]

Synonym: Ppro_2595

Alternate gene names: 118581007

Gene position: 2817006-2818490 (Clockwise)

Preceding gene: 118580995

Following gene: 118581008

Centisome position: 70.28

GC content: 66.46

Gene sequence:

>1485_bases
ATGAGCGACTCAGGCAAACCGTTCCGTCTCGTCGTCAACAGGAACACGCATTCCTCGCCCATCACGGGAGTCTATCTGGT
TACCGACCAGGACGACGATCTGGTAGAACGGGTGTCGACGGCCCTCAAGGGGGGCGTCTCGGCTCTGCAGTATCGCGCCA
AGGGGAAGGAGCGTTGCCACTGCCTCTCCGAGGCCGCCGAACTGAAGCGACTCTGCCGCGACTTCGGGGTGGCCTTCATC
GTCAACGACGACATGCTGCTGGCCAAGGTACTGGAGGCGGACGGCGTCCACCTGGGTCAGGACGACGGCAGCGTTGCCCA
GGCCCGCGCCCTGCTGGGCCCGGACAGCATCATCGGCAAATCGACCCACGACCTGCGGGAGGCCTTGGAGGCGGAAGCCG
AGGGGGCCGACTACATCGGATTCGGTTCCATGTATCCCACCACCAGCAAATCGGTCAGCCACCTGCCGGGGACGACCGGC
CTGATGGAGATCCGCGACCGGATCAGGCTCCCCATCGTGGCCATCGGCGGCATCACCCCCGCCAATGCCTGTCGGGTGGT
GGATGCTGGCGCCGACGCCCTGGCGGTGATCTCCTCGGTGCTCTCCTCCCCGCGGCCGGACGTGGCTGTGACGGAACTAA
AGCTGCTCTTCAACCGCAGGCGCCCCCTGCCCCGCGGCGCGGTGCTGACGGTGGCCGGCAGCGATTCCGGCGGCGGCGCG
GGCATCCAGGCCGACATCAAGACCATCACCCTGTTGGGGAGTTATGCCGCCAGCGTGCTCACCGCCCTGACCGCCCAGAA
CACCCGCGGTGTTTCATCCATCCACGGCCTGCCCCCCTCCTTCGTCATGGATCAGCTGGACAGCGTGCTGGCGGACATCC
CCATCGACGTGATAAAGACCGGGATGCTGCATACGCCGGCCATCGTCTCGGCCTTGGCGGAACGGCTAGGGGAACGGACC
ACCCTGCTGCCGCTGGTGATCGACCCGGTCATGATCGCCAAGGGGGGAGCGGCCCTGATGGAGCGCGAGGCGGCCCAGAC
CTTTCTGGAGCAGTTGATGCCGCTGGCCTACCTGCTCACCCCCAATATCCCCGAGACGGAGCGCCTCCTGGGGAGAACGA
TCCAGAACGAGGCCGAAACCGAGCAGGCTGCCCGGGACCTGCATGCCCTGGGAGCGGCCAATGTGCTGATCAAGGGGGGG
CACATGGGTGGCAGGCTCTCCACCGACATCCTCTTCGACGGCAGAGAATGCCATCACTTCAGCGTCGAGCGGGTATTCAC
CAGCAACACCCACGGCACCGGCTGCACCTACGCCTCGGCCATAGCCGCATTCCTGGCCCAGGGAGAACCGCTGCGGACGG
CCGTGGAACGGGCCAAGCAGTTCATCACCGCAGCGATTCAGCTGGCCCGCCCCCTGGGCAGGGGACACAGCCCGGTCAAC
CATTTCGCGGCGGCCCAGCAGACCGCAGCGCATCCACTATCCTAG

Upstream 100 bases:

>100_bases
TAGGAAAGCGGCTCGCGTGACGATTCCATGAATCCAGCCGCCTGATTATGTTCCATCAGAGCGGCTTTTTTGTTTCCGGC
CAGTAAAAAGGATACAGGCA

Downstream 100 bases:

>100_bases
CGTTATTTTTATGTCTGAAAACGGTAGATGGGAGCTGCCGGAGAGAGCGCATCAGGGCTCTTTCGTCGTAATTACCGACG
AAATGGAGCGCGAACACAGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSDSGKPFRLVVNRNTHSSPITGVYLVTDQDDDLVERVSTALKGGVSALQYRAKGKERCHCLSEAAELKRLCRDFGVAFI
VNDDMLLAKVLEADGVHLGQDDGSVAQARALLGPDSIIGKSTHDLREALEAEAEGADYIGFGSMYPTTSKSVSHLPGTTG
LMEIRDRIRLPIVAIGGITPANACRVVDAGADALAVISSVLSSPRPDVAVTELKLLFNRRRPLPRGAVLTVAGSDSGGGA
GIQADIKTITLLGSYAASVLTALTAQNTRGVSSIHGLPPSFVMDQLDSVLADIPIDVIKTGMLHTPAIVSALAERLGERT
TLLPLVIDPVMIAKGGAALMEREAAQTFLEQLMPLAYLLTPNIPETERLLGRTIQNEAETEQAARDLHALGAANVLIKGG
HMGGRLSTDILFDGRECHHFSVERVFTSNTHGTGCTYASAIAAFLAQGEPLRTAVERAKQFITAAIQLARPLGRGHSPVN
HFAAAQQTAAHPLS

Sequences:

>Translated_494_residues
MSDSGKPFRLVVNRNTHSSPITGVYLVTDQDDDLVERVSTALKGGVSALQYRAKGKERCHCLSEAAELKRLCRDFGVAFI
VNDDMLLAKVLEADGVHLGQDDGSVAQARALLGPDSIIGKSTHDLREALEAEAEGADYIGFGSMYPTTSKSVSHLPGTTG
LMEIRDRIRLPIVAIGGITPANACRVVDAGADALAVISSVLSSPRPDVAVTELKLLFNRRRPLPRGAVLTVAGSDSGGGA
GIQADIKTITLLGSYAASVLTALTAQNTRGVSSIHGLPPSFVMDQLDSVLADIPIDVIKTGMLHTPAIVSALAERLGERT
TLLPLVIDPVMIAKGGAALMEREAAQTFLEQLMPLAYLLTPNIPETERLLGRTIQNEAETEQAARDLHALGAANVLIKGG
HMGGRLSTDILFDGRECHHFSVERVFTSNTHGTGCTYASAIAAFLAQGEPLRTAVERAKQFITAAIQLARPLGRGHSPVN
HFAAAQQTAAHPLS
>Mature_493_residues
SDSGKPFRLVVNRNTHSSPITGVYLVTDQDDDLVERVSTALKGGVSALQYRAKGKERCHCLSEAAELKRLCRDFGVAFIV
NDDMLLAKVLEADGVHLGQDDGSVAQARALLGPDSIIGKSTHDLREALEAEAEGADYIGFGSMYPTTSKSVSHLPGTTGL
MEIRDRIRLPIVAIGGITPANACRVVDAGADALAVISSVLSSPRPDVAVTELKLLFNRRRPLPRGAVLTVAGSDSGGGAG
IQADIKTITLLGSYAASVLTALTAQNTRGVSSIHGLPPSFVMDQLDSVLADIPIDVIKTGMLHTPAIVSALAERLGERTT
LLPLVIDPVMIAKGGAALMEREAAQTFLEQLMPLAYLLTPNIPETERLLGRTIQNEAETEQAARDLHALGAANVLIKGGH
MGGRLSTDILFDGRECHHFSVERVFTSNTHGTGCTYASAIAAFLAQGEPLRTAVERAKQFITAAIQLARPLGRGHSPVNH
FAAAQQTAAHPLS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=265, Percent_Identity=44.5283018867925, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790426, Length=194, Percent_Identity=31.4432989690722, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325378, Length=288, Percent_Identity=37.1527777777778, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324997, Length=301, Percent_Identity=37.5415282392027, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324517, Length=289, Percent_Identity=38.7543252595156, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325042, Length=222, Percent_Identity=32.4324324324324, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 52257; Mature: 52126

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSGKPFRLVVNRNTHSSPITGVYLVTDQDDDLVERVSTALKGGVSALQYRAKGKERCH
CCCCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
CLSEAAELKRLCRDFGVAFIVNDDMLLAKVLEADGVHLGQDDGSVAQARALLGPDSIIGK
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHHCCC
STHDLREALEAEAEGADYIGFGSMYPTTSKSVSHLPGTTGLMEIRDRIRLPIVAIGGITP
CHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEECCCCC
ANACRVVDAGADALAVISSVLSSPRPDVAVTELKLLFNRRRPLPRGAVLTVAGSDSGGGA
CCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
GIQADIKTITLLGSYAASVLTALTAQNTRGVSSIHGLPPSFVMDQLDSVLADIPIDVIKT
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHC
GMLHTPAIVSALAERLGERTTLLPLVIDPVMIAKGGAALMEREAAQTFLEQLMPLAYLLT
CCCCCHHHHHHHHHHHCCCCEEHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PNIPETERLLGRTIQNEAETEQAARDLHALGAANVLIKGGHMGGRLSTDILFDGRECHHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCE
SVERVFTSNTHGTGCTYASAIAAFLAQGEPLRTAVERAKQFITAAIQLARPLGRGHSPVN
EHHHEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
HFAAAQQTAAHPLS
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SDSGKPFRLVVNRNTHSSPITGVYLVTDQDDDLVERVSTALKGGVSALQYRAKGKERCH
CCCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
CLSEAAELKRLCRDFGVAFIVNDDMLLAKVLEADGVHLGQDDGSVAQARALLGPDSIIGK
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCEECCCCCHHHHHHHHCCCHHHCCC
STHDLREALEAEAEGADYIGFGSMYPTTSKSVSHLPGTTGLMEIRDRIRLPIVAIGGITP
CHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCEEEECCCCC
ANACRVVDAGADALAVISSVLSSPRPDVAVTELKLLFNRRRPLPRGAVLTVAGSDSGGGA
CCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
GIQADIKTITLLGSYAASVLTALTAQNTRGVSSIHGLPPSFVMDQLDSVLADIPIDVIKT
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHC
GMLHTPAIVSALAERLGERTTLLPLVIDPVMIAKGGAALMEREAAQTFLEQLMPLAYLLT
CCCCCHHHHHHHHHHHCCCCEEHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PNIPETERLLGRTIQNEAETEQAARDLHALGAANVLIKGGHMGGRLSTDILFDGRECHHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCE
SVERVFTSNTHGTGCTYASAIAAFLAQGEPLRTAVERAKQFITAAIQLARPLGRGHSPVN
EHHHEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
HFAAAQQTAAHPLS
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA