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The map label for this gene is yhcK [H]

Identifier: 118580910

GI number: 118580910

Start: 2705490

End: 2706557

Strand: Reverse

Name: yhcK [H]

Synonym: Ppro_2497

Alternate gene names: 118580910

Gene position: 2706557-2705490 (Counterclockwise)

Preceding gene: 118580913

Following gene: 118580909

Centisome position: 67.53

GC content: 62.27

Gene sequence:

>1068_bases
ATGTCAGCAGCAACCGATTGTGACAAATGCAAGTCGTATGAGCTCAAAGGGCAGGTGCGTGCTCTGAACGACCTGCTCAA
TGTGGCTCAGGCCTCCATCGCCTCCCTGGAGCTTGCCCCCCTGACGCAGACCATCCTGCGGGGCGCCATGGATTTCATGG
AGATGCCGGTGGGCAGGCTGGCGCTGTACGACAAGCGCAACGGCAGGATGGTGCTGCGCGCCCATGCCGGTCTGGACGAG
GATTGCGTCGTGCGATCGGTCTGGACGGTGGCGCCGGGAGGCCTGACGGACCGGCTTCTTAGGCGCAATGAGGTCTACTA
TGTTGAGGATAGGGAGGCGAGCCAGATACCGGAGGAGCACCTGATCGCCCATGAGCAGAGCCGCTCGCTGGTTGCGGTTC
CGCTGGTCTCCCAGAGCTCCATCCACGGCATGCTCTGCCTGGATGACGTCATACCGCGCACCTTCGACAAGAACCGCATC
TCGCAGATCCTGGTTCTGGCCTCCTTTGCCGCCATATCCATCGAGAACGCCAAGTTTCACGAGGAAACCCACCAGCTGGC
CATCAACGACTCCCTCACCGGACTGAACAACCGGCGCTACTTCGACAAGGTCCTGCCCCAGGAGTATGAACGGGCGCACC
GGGAGGGGCTCCCCTTCTCCCTGATGATGATCGACGTGGACAACTTCAAGCTGTTCAACGACACCCATGGTCACCACCTG
GGTGACCGCATCCTGGCCACCATCGGGCGGGTGCTGCGCAAAACCCTGCGCAGTATCGATTTTGCCTTCCGCTACGGCGG
AGAGGAGTTCGTGGTCATCCTGCCGGAAACCGACCTGGAGAGCGCCCACAAGGTGGCCGAGCGCATCAGGCAGCGGGTCA
TGGCGGAGAGCAGGAAGCTGTTGCGCAACCCGCAGGACAGGCCGGTTACGGTGAGTGTGGGCATATCCTGCTATCCCCGT
GACGCTGCCTGTTCCATTACCCTGCTGGCCATGGCCGACCAGCTGCTCTACCAGGCCAAGCGGACTGGGCGGAACCGCGT
CCTGGTGGCGGAGGATGATGCCGCTTGA

Upstream 100 bases:

>100_bases
GCATGGGTTAAAAAAACAGGGGATAAGTTTAAAATATTTGCATGAAAGCTTAAATGTGCTAAAAACAAGACTCGATATGT
ATGAAGGAGTATGCGGGACG

Downstream 100 bases:

>100_bases
TGATTCTGTTTCGCACCCCCACCCATCGCCTGTTCGTGGCCTGTATCCTGCTCTTTCCCCTGCTGTCCGCCTGCACCGCT
TTCTCCGCATGGGATACATC

Product: GAF sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSAATDCDKCKSYELKGQVRALNDLLNVAQASIASLELAPLTQTILRGAMDFMEMPVGRLALYDKRNGRMVLRAHAGLDE
DCVVRSVWTVAPGGLTDRLLRRNEVYYVEDREASQIPEEHLIAHEQSRSLVAVPLVSQSSIHGMLCLDDVIPRTFDKNRI
SQILVLASFAAISIENAKFHEETHQLAINDSLTGLNNRRYFDKVLPQEYERAHREGLPFSLMMIDVDNFKLFNDTHGHHL
GDRILATIGRVLRKTLRSIDFAFRYGGEEFVVILPETDLESAHKVAERIRQRVMAESRKLLRNPQDRPVTVSVGISCYPR
DAACSITLLAMADQLLYQAKRTGRNRVLVAEDDAA

Sequences:

>Translated_355_residues
MSAATDCDKCKSYELKGQVRALNDLLNVAQASIASLELAPLTQTILRGAMDFMEMPVGRLALYDKRNGRMVLRAHAGLDE
DCVVRSVWTVAPGGLTDRLLRRNEVYYVEDREASQIPEEHLIAHEQSRSLVAVPLVSQSSIHGMLCLDDVIPRTFDKNRI
SQILVLASFAAISIENAKFHEETHQLAINDSLTGLNNRRYFDKVLPQEYERAHREGLPFSLMMIDVDNFKLFNDTHGHHL
GDRILATIGRVLRKTLRSIDFAFRYGGEEFVVILPETDLESAHKVAERIRQRVMAESRKLLRNPQDRPVTVSVGISCYPR
DAACSITLLAMADQLLYQAKRTGRNRVLVAEDDAA
>Mature_354_residues
SAATDCDKCKSYELKGQVRALNDLLNVAQASIASLELAPLTQTILRGAMDFMEMPVGRLALYDKRNGRMVLRAHAGLDED
CVVRSVWTVAPGGLTDRLLRRNEVYYVEDREASQIPEEHLIAHEQSRSLVAVPLVSQSSIHGMLCLDDVIPRTFDKNRIS
QILVLASFAAISIENAKFHEETHQLAINDSLTGLNNRRYFDKVLPQEYERAHREGLPFSLMMIDVDNFKLFNDTHGHHLG
DRILATIGRVLRKTLRSIDFAFRYGGEEFVVILPETDLESAHKVAERIRQRVMAESRKLLRNPQDRPVTVSVGISCYPRD
AACSITLLAMADQLLYQAKRTGRNRVLVAEDDAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=194, Percent_Identity=38.659793814433, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=38.2352941176471, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=38.0681818181818, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=35.6382978723404, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=38.4615384615385, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.1463414634146, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788381, Length=205, Percent_Identity=31.7073170731707, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=30.4093567251462, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=175, Percent_Identity=30.8571428571429, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87081974, Length=106, Percent_Identity=33.9622641509434, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787541, Length=189, Percent_Identity=29.1005291005291, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=231, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=31.4465408805031, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 39983; Mature: 39852

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATDCDKCKSYELKGQVRALNDLLNVAQASIASLELAPLTQTILRGAMDFMEMPVGRL
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCE
ALYDKRNGRMVLRAHAGLDEDCVVRSVWTVAPGGLTDRLLRRNEVYYVEDREASQIPEEH
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHCCHHH
LIAHEQSRSLVAVPLVSQSSIHGMLCLDDVIPRTFDKNRISQILVLASFAAISIENAKFH
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCHHH
EETHQLAINDSLTGLNNRRYFDKVLPQEYERAHREGLPFSLMMIDVDNFKLFNDTHGHHL
HHHHHEEECCCCCCCCCCHHHHHHCHHHHHHHHHCCCCEEEEEEEECCEEEECCCCCCHH
GDRILATIGRVLRKTLRSIDFAFRYGGEEFVVILPETDLESAHKVAERIRQRVMAESRKL
HHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LRNPQDRPVTVSVGISCYPRDAACSITLLAMADQLLYQAKRTGRNRVLVAEDDAA
HCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure 
SAATDCDKCKSYELKGQVRALNDLLNVAQASIASLELAPLTQTILRGAMDFMEMPVGRL
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCE
ALYDKRNGRMVLRAHAGLDEDCVVRSVWTVAPGGLTDRLLRRNEVYYVEDREASQIPEEH
EEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHCCHHH
LIAHEQSRSLVAVPLVSQSSIHGMLCLDDVIPRTFDKNRISQILVLASFAAISIENAKFH
HHHHHCCCCEEEEEECCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCHHH
EETHQLAINDSLTGLNNRRYFDKVLPQEYERAHREGLPFSLMMIDVDNFKLFNDTHGHHL
HHHHHEEECCCCCCCCCCHHHHHHCHHHHHHHHHCCCCEEEEEEEECCEEEECCCCCCHH
GDRILATIGRVLRKTLRSIDFAFRYGGEEFVVILPETDLESAHKVAERIRQRVMAESRKL
HHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LRNPQDRPVTVSVGISCYPRDAACSITLLAMADQLLYQAKRTGRNRVLVAEDDAA
HCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]