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The map label for this gene is vipB [H]

Identifier: 118580853

GI number: 118580853

Start: 2638275

End: 2639363

Strand: Reverse

Name: vipB [H]

Synonym: Ppro_2440

Alternate gene names: 118580853

Gene position: 2639363-2638275 (Counterclockwise)

Preceding gene: 118580854

Following gene: 118580852

Centisome position: 65.85

GC content: 63.73

Gene sequence:

>1089_bases
ATGAACCGCTACGATATCGTACGAGAACAGCTGAAACAGAATCCCCGAACCTGGCTGGTGACGGGAGTGGCCGGCTTCAT
CGGCTCCAACCTGCTGGAGAGCCTGCTTACGCTTGGCCAGCGGGTGGTGGGGCTGGACAACTTCTCCACCGGCAAGCGCG
CCAACCTGGATGAAGTCAGCCGGGTGGTGACGCCGCGGCAGTGGGCTGCCTTCACCTTCCATGAAGGGGATATCCGCGAG
CTCGCCGCCTGCCGCGCCGCCTGTCAGGGGGTCGACTATATCCTGCACGAGGCTGCCCTCGGTTCGGTGCCCCGCTCCAT
CGAGGACCCGATCAGCAGCAACGAGAGCAATGTCGGCGGATTCCTGAACATGCTGGTGGCTGCCCGGGATGCCAAGGTGC
GGCGAATGGTCTACGCGGCCAGCAGTTCCACCTATGGCGACCACCCCGGCCTGCCCAAGCGGGAGGAGCTGATCGGCAGG
CCGCTCTCCCCCTACGCGGTCACCAAGTACGTGAACGAACTGTACGCCGATGTTTTTGCCCGGACCTATGCCATGGAACT
GGTAGGGCTGCGCTACTTCAACGTCTTCGGCCCGCGCCAGGACCCGGAAGGGGCCTATGCGGCGGTGATCCCGCGCTGGA
TCAGGTCCATGATCATCGACGAGACCGTGTATATCAACGGCAGCGGAGAGACGAGCAGGGATTTCTGCTTCGTGGAGAAC
GTGATCCAGATGAACCTGCTGGCAGCCACGGCTGATGAACCGCAGGCGGTCAACCAGGTGTACAACACCGCGGTCAACGC
CCGCACCACCCTGAATGAGCTCTACGACATGCTTCGGCAGCGACTCGTCCCCCGTTTCCCCCATCTTGCCCATCGCACGC
CGCTGCACCGGGATTTCCGGGCAGGGGACGTGCTGCATTCCCAGGCCGATATTTCCAAGGCTGCCCGGCTTCTGGGGTAT
CAGCCGACCCACACCATCCAGAGCGGGCTGGACGCCTCTCTATCGTGGTACGTGGATAACCTCTGCCCCACGGTGGAGGG
GAACTTGCCCGCTGCCGGGTCACGGGGCCTGGAGCCCGTGGGGAACTGA

Upstream 100 bases:

>100_bases
TGGAGGCTGTGAGGTGGGGAACAGGGAGTCGCCGCCTCCTGATCACTGCCCACTGCCCGCTGCCCGCTGAACGCTGAACG
CTGAACGCTGAAGGAATGAC

Downstream 100 bases:

>100_bases
CATGCTGGACTCGGTCATCACCATCGCGGCCATTGTTGCCCTGCTGTTTGCCGCGGGATACGCCCTGCACCGGGAACGCT
CCCGCGCCGGCCTGTTTCTC

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNRYDIVREQLKQNPRTWLVTGVAGFIGSNLLESLLTLGQRVVGLDNFSTGKRANLDEVSRVVTPRQWAAFTFHEGDIRE
LAACRAACQGVDYILHEAALGSVPRSIEDPISSNESNVGGFLNMLVAARDAKVRRMVYAASSSTYGDHPGLPKREELIGR
PLSPYAVTKYVNELYADVFARTYAMELVGLRYFNVFGPRQDPEGAYAAVIPRWIRSMIIDETVYINGSGETSRDFCFVEN
VIQMNLLAATADEPQAVNQVYNTAVNARTTLNELYDMLRQRLVPRFPHLAHRTPLHRDFRAGDVLHSQADISKAARLLGY
QPTHTIQSGLDASLSWYVDNLCPTVEGNLPAAGSRGLEPVGN

Sequences:

>Translated_362_residues
MNRYDIVREQLKQNPRTWLVTGVAGFIGSNLLESLLTLGQRVVGLDNFSTGKRANLDEVSRVVTPRQWAAFTFHEGDIRE
LAACRAACQGVDYILHEAALGSVPRSIEDPISSNESNVGGFLNMLVAARDAKVRRMVYAASSSTYGDHPGLPKREELIGR
PLSPYAVTKYVNELYADVFARTYAMELVGLRYFNVFGPRQDPEGAYAAVIPRWIRSMIIDETVYINGSGETSRDFCFVEN
VIQMNLLAATADEPQAVNQVYNTAVNARTTLNELYDMLRQRLVPRFPHLAHRTPLHRDFRAGDVLHSQADISKAARLLGY
QPTHTIQSGLDASLSWYVDNLCPTVEGNLPAAGSRGLEPVGN
>Mature_362_residues
MNRYDIVREQLKQNPRTWLVTGVAGFIGSNLLESLLTLGQRVVGLDNFSTGKRANLDEVSRVVTPRQWAAFTFHEGDIRE
LAACRAACQGVDYILHEAALGSVPRSIEDPISSNESNVGGFLNMLVAARDAKVRRMVYAASSSTYGDHPGLPKREELIGR
PLSPYAVTKYVNELYADVFARTYAMELVGLRYFNVFGPRQDPEGAYAAVIPRWIRSMIIDETVYINGSGETSRDFCFVEN
VIQMNLLAATADEPQAVNQVYNTAVNARTTLNELYDMLRQRLVPRFPHLAHRTPLHRDFRAGDVLHSQADISKAARLLGY
QPTHTIQSGLDASLSWYVDNLCPTVEGNLPAAGSRGLEPVGN

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=351, Percent_Identity=26.4957264957265, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=25.4545454545455, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56237023, Length=334, Percent_Identity=25.748502994012, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI56118217, Length=334, Percent_Identity=25.748502994012, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI189083684, Length=334, Percent_Identity=25.748502994012, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=27.9761904761905, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=29.4285714285714, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=27.6657060518732, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790049, Length=323, Percent_Identity=24.7678018575851, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=350, Percent_Identity=24.2857142857143, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=26.7045454545455, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=26.4204545454545, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=25.3687315634218, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=25.6906077348066, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=350, Percent_Identity=24.5714285714286, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=28.448275862069, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 40145; Mature: 40145

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYDIVREQLKQNPRTWLVTGVAGFIGSNLLESLLTLGQRVVGLDNFSTGKRANLDEVS
CCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RVVTPRQWAAFTFHEGDIRELAACRAACQGVDYILHEAALGSVPRSIEDPISSNESNVGG
HHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHH
FLNMLVAARDAKVRRMVYAASSSTYGDHPGLPKREELIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RTYAMELVGLRYFNVFGPRQDPEGAYAAVIPRWIRSMIIDETVYINGSGETSRDFCFVEN
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
VIQMNLLAATADEPQAVNQVYNTAVNARTTLNELYDMLRQRLVPRFPHLAHRTPLHRDFR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
AGDVLHSQADISKAARLLGYQPTHTIQSGLDASLSWYVDNLCPTVEGNLPAAGSRGLEPV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GN
CC
>Mature Secondary Structure
MNRYDIVREQLKQNPRTWLVTGVAGFIGSNLLESLLTLGQRVVGLDNFSTGKRANLDEVS
CCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RVVTPRQWAAFTFHEGDIRELAACRAACQGVDYILHEAALGSVPRSIEDPISSNESNVGG
HHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHH
FLNMLVAARDAKVRRMVYAASSSTYGDHPGLPKREELIGRPLSPYAVTKYVNELYADVFA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RTYAMELVGLRYFNVFGPRQDPEGAYAAVIPRWIRSMIIDETVYINGSGETSRDFCFVEN
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
VIQMNLLAATADEPQAVNQVYNTAVNARTTLNELYDMLRQRLVPRFPHLAHRTPLHRDFR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
AGDVLHSQADISKAARLLGYQPTHTIQSGLDASLSWYVDNLCPTVEGNLPAAGSRGLEPV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]