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The map label for this gene is rph [H]

Identifier: 118580726

GI number: 118580726

Start: 2493663

End: 2494382

Strand: Reverse

Name: rph [H]

Synonym: Ppro_2311

Alternate gene names: 118580726

Gene position: 2494382-2493663 (Counterclockwise)

Preceding gene: 118580727

Following gene: 118580725

Centisome position: 62.24

GC content: 62.5

Gene sequence:

>720_bases
ATGGTGCGGAAAAACGGAAGGGACAGTCGGGCATTGCGGCCGGTTGCCATAACCAGAAACTTTACCAAGTATGCCGAGGG
GGCGGTTCTGGTGGAGTTCGGTGATACCAGGGTGATCTGTACCGCCTCGGTTGAGGAATCGGTGCCCTCCTTTTTGCGCG
GCAGGGGAACCGGGTGGGTGACCGCCGAGTATTCCATGCTGCCGCGGGCAACCCATACCCGTTCGTCCCGCGAAGCTTCC
AAGGGAAAACAGAGCGGCCGCACGCTGGAAATCCAGCGCCTGATCGGCCGCTCGCTGCGGGCGGTCACCGATCTGGCGGC
ACTGGGTGAGCGGAGTGTCTATCTCGACTGCGATGTGCTCCAGGCCGATGGCGGCACCAGAACAGCTTCCATAACCGGCG
CCTACGTGGCGCTGGCCGATGCCCTGGCAACACTGAAGCAGCGGGGGCTCTTGGAGCGGATCCCGCTCAGGGAGGCTGTT
GCGGCGGTGAGCGTGGGTATCGTGGGGGGAGAGAGCCTGCTGGACCTGGATTACGACGAGGACTCCTCAGCCGAGGTGGA
CATGAATTTCGTCATGACTTCCAGTCAGCGTTTCGTCGAGGTGCAGGGGACCGCTGAGGTGGAGCCGTTCACCCTTGAGC
AGATGGACGCCATGCGCAGTCTGGCCATGGACGGCATTCAGTGTCTGTTCGATCTTCAGCGGGAGGCGCTCAAGGGATGA

Upstream 100 bases:

>100_bases
TTATGGAACACGCTTCAATTTTATCGACACAGTTCGTGATTGCGACTGGCGGTTGGGGAGGTCTTCAACTGTTCAATCCG
GTTTATGGAGGGAGTTCAGC

Downstream 100 bases:

>100_bases
AAGAACTGGTGGTGGCCACTCGCAATCGCGGAAAACTGAACGAGATACGGGCGTTCCTGTCGGGTGTCGTGGAACGGGTC
AGTTGCGCTTCCGACTTCGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MVRKNGRDSRALRPVAITRNFTKYAEGAVLVEFGDTRVICTASVEESVPSFLRGRGTGWVTAEYSMLPRATHTRSSREAS
KGKQSGRTLEIQRLIGRSLRAVTDLAALGERSVYLDCDVLQADGGTRTASITGAYVALADALATLKQRGLLERIPLREAV
AAVSVGIVGGESLLDLDYDEDSSAEVDMNFVMTSSQRFVEVQGTAEVEPFTLEQMDAMRSLAMDGIQCLFDLQREALKG

Sequences:

>Translated_239_residues
MVRKNGRDSRALRPVAITRNFTKYAEGAVLVEFGDTRVICTASVEESVPSFLRGRGTGWVTAEYSMLPRATHTRSSREAS
KGKQSGRTLEIQRLIGRSLRAVTDLAALGERSVYLDCDVLQADGGTRTASITGAYVALADALATLKQRGLLERIPLREAV
AAVSVGIVGGESLLDLDYDEDSSAEVDMNFVMTSSQRFVEVQGTAEVEPFTLEQMDAMRSLAMDGIQCLFDLQREALKG
>Mature_239_residues
MVRKNGRDSRALRPVAITRNFTKYAEGAVLVEFGDTRVICTASVEESVPSFLRGRGTGWVTAEYSMLPRATHTRSSREAS
KGKQSGRTLEIQRLIGRSLRAVTDLAALGERSVYLDCDVLQADGGTRTASITGAYVALADALATLKQRGLLERIPLREAV
AAVSVGIVGGESLLDLDYDEDSSAEVDMNFVMTSSQRFVEVQGTAEVEPFTLEQMDAMRSLAMDGIQCLFDLQREALKG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=67.6190476190476, Blast_Score=298, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71981632, Length=244, Percent_Identity=23.7704918032787, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25987; Mature: 25987

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKNGRDSRALRPVAITRNFTKYAEGAVLVEFGDTRVICTASVEESVPSFLRGRGTGWV
CCCCCCCCCCCCCEEEECCCHHHHHCCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCEE
TAEYSMLPRATHTRSSREASKGKQSGRTLEIQRLIGRSLRAVTDLAALGERSVYLDCDVL
EEEHHHCCCCHHCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
QADGGTRTASITGAYVALADALATLKQRGLLERIPLREAVAAVSVGIVGGESLLDLDYDE
ECCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHEEECCCEEEECCCCC
DSSAEVDMNFVMTSSQRFVEVQGTAEVEPFTLEQMDAMRSLAMDGIQCLFDLQREALKG
CCCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVRKNGRDSRALRPVAITRNFTKYAEGAVLVEFGDTRVICTASVEESVPSFLRGRGTGWV
CCCCCCCCCCCCCEEEECCCHHHHHCCEEEEEECCCEEEEECCHHHHHHHHHHCCCCCEE
TAEYSMLPRATHTRSSREASKGKQSGRTLEIQRLIGRSLRAVTDLAALGERSVYLDCDVL
EEEHHHCCCCHHCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
QADGGTRTASITGAYVALADALATLKQRGLLERIPLREAVAAVSVGIVGGESLLDLDYDE
ECCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHEEECCCEEEECCCCC
DSSAEVDMNFVMTSSQRFVEVQGTAEVEPFTLEQMDAMRSLAMDGIQCLFDLQREALKG
CCCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA