Click here to switch to the map view.

The map label for this gene is ackA [H]

Identifier: 118580645

GI number: 118580645

Start: 2404671

End: 2405924

Strand: Direct

Name: ackA [H]

Synonym: Ppro_2230

Alternate gene names: 118580645

Gene position: 2404671-2405924 (Clockwise)

Preceding gene: 118580643

Following gene: 118580647

Centisome position: 60.0

GC content: 61.16

Gene sequence:

>1254_bases
ATGGCTTTAAACTGCGGCAGTTCGTCGGTCAAGTACCAGCTTTTCGATTGGAATCAGAAAGCGGTCGTGGCCAAGGGTAT
GGTTGAGCGGGTCACCATCGATGATTCCTACATCGTGCATGAGGTCCCCGGGCGTGAGGTGTACAAGGATGAGCACAACT
GTCCCACCCATCAGGCCGCCATCGACCTGATCATCAGGACGGTCACCGATTCCGAGCAGGGCGTCATCAAGAACGTCTCC
GAAATCGCGGCCGTCGGCCACCGCCTGGTGCATGGCGGAGAGAAATTCACCTGTTCCGTCGTAATCGACGAGAACGTTCT
GGAGACGGTCAAGGAGTGCCAGCACCTGGCGCCGCTGCACAACCCGCCCAACATCGAGGGGGTCCAGGCTGCCCGTGCGC
TGCTGCCCACCGTTCCCCAGGTGGCGGTCTTCGACACCGCCTTCCATCAGACCATGCCGGAACAGGCCTACATCTATCCG
GTTCCCTACGAGTGGTACCAGAAGCACCAGGTCCGTCGCTACGGCTTCCACGGCACCTCCCACCTGTACGTCGCCAAGCG
CGCGGCGGCCCTTTTGGGCAAGAAACCGGCCGACACCAACCTGGTTACCCTGCACATCGGCAACGGCGTCTCCCACTGCG
CCATCAAGAATGGCATCTCCATCGACACCTCCATGGGGCTCACCCCGCTGGAAGGGGCCATCATGGGCACCCGTTGCGGC
GACATCGACCCGGCCATCCCGCTGTTCATCATGCAGAAGGAGGGTTTGAACGCCAAGGAGATCGACACCATCCTGAACAA
GAAGAGCGGCATCATGGGCATCACCGGCCGTTTCTCCGACCGCCGCGACGTGTCCCATCATGCCGAGACCGGCGACAAGC
TCTGCAAGCTGGCCATGGACATCGAGGCCTACCGCATCAAGAAGTACATCGGCGCCTACATGGCTGCCGTGGGCAAACTG
GACGCAGTCGTCTTCACCGCCGGCGTGGGCGAGATGAGCGTTGAGACCCGCGAGATGGTTCTGGAAGGAATGGAGCATCT
GGGGCTGATCCTGGACAAGGAGCGCAACAAGGTGCGCACCCGCAAGAAAGAGACCCTGATCACCACCGACGACTCCCCGG
TCAAGGCCTTCGTCATCCCCACCGACGAGGAGCTGGTCCTGACCGAGGATGTGGCTGCCATCCTGAACGGCACCTACGCC
GACCACATGAACTTCGAGTACTCCTTCTCCCGGCCGGATTTCGTACGCAAGTAG

Upstream 100 bases:

>100_bases
GGCATACTGTATACAATTGGAGTTGCAAACCTGTTGAATAATACTTCGCTTTGTTGTATACAATTGAAAGTTTATTTCAG
GAGGATACAACGTGATAATA

Downstream 100 bases:

>100_bases
TCCGGACACTAGACAGGTCACACGGAAAAGCCGGCGTTAACGCCGGCTTTTCTCGTTTCAGACCGATTTCATGCGACTGC
TGTGGCAGCCTTACCCCTTC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MALNCGSSSVKYQLFDWNQKAVVAKGMVERVTIDDSYIVHEVPGREVYKDEHNCPTHQAAIDLIIRTVTDSEQGVIKNVS
EIAAVGHRLVHGGEKFTCSVVIDENVLETVKECQHLAPLHNPPNIEGVQAARALLPTVPQVAVFDTAFHQTMPEQAYIYP
VPYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGTRCG
DIDPAIPLFIMQKEGLNAKEIDTILNKKSGIMGITGRFSDRRDVSHHAETGDKLCKLAMDIEAYRIKKYIGAYMAAVGKL
DAVVFTAGVGEMSVETREMVLEGMEHLGLILDKERNKVRTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYA
DHMNFEYSFSRPDFVRK

Sequences:

>Translated_417_residues
MALNCGSSSVKYQLFDWNQKAVVAKGMVERVTIDDSYIVHEVPGREVYKDEHNCPTHQAAIDLIIRTVTDSEQGVIKNVS
EIAAVGHRLVHGGEKFTCSVVIDENVLETVKECQHLAPLHNPPNIEGVQAARALLPTVPQVAVFDTAFHQTMPEQAYIYP
VPYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGTRCG
DIDPAIPLFIMQKEGLNAKEIDTILNKKSGIMGITGRFSDRRDVSHHAETGDKLCKLAMDIEAYRIKKYIGAYMAAVGKL
DAVVFTAGVGEMSVETREMVLEGMEHLGLILDKERNKVRTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYA
DHMNFEYSFSRPDFVRK
>Mature_416_residues
ALNCGSSSVKYQLFDWNQKAVVAKGMVERVTIDDSYIVHEVPGREVYKDEHNCPTHQAAIDLIIRTVTDSEQGVIKNVSE
IAAVGHRLVHGGEKFTCSVVIDENVLETVKECQHLAPLHNPPNIEGVQAARALLPTVPQVAVFDTAFHQTMPEQAYIYPV
PYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGTRCGD
IDPAIPLFIMQKEGLNAKEIDTILNKKSGIMGITGRFSDRRDVSHHAETGDKLCKLAMDIEAYRIKKYIGAYMAAVGKLD
AVVFTAGVGEMSVETREMVLEGMEHLGLILDKERNKVRTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYAD
HMNFEYSFSRPDFVRK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=42.8211586901763, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=38.1679389312977, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 46190; Mature: 46058

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNCGSSSVKYQLFDWNQKAVVAKGMVERVTIDDSYIVHEVPGREVYKDEHNCPTHQAA
CEECCCCCCEEEEEEECCCCHHHHCCCEEEEEECCCEEEEECCCCHHHCCCCCCCHHHHH
IDLIIRTVTDSEQGVIKNVSEIAAVGHRLVHGGEKFTCSVVIDENVLETVKECQHLAPLH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCCC
NPPNIEGVQAARALLPTVPQVAVFDTAFHQTMPEQAYIYPVPYEWYQKHQVRRYGFHGTS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCC
HLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGTRCG
EEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCCCCCCEEECCCC
DIDPAIPLFIMQKEGLNAKEIDTILNKKSGIMGITGRFSDRRDVSHHAETGDKLCKLAMD
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IEAYRIKKYIGAYMAAVGKLDAVVFTAGVGEMSVETREMVLEGMEHLGLILDKERNKVRT
HHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEECCHHHHHH
RKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYADHMNFEYSFSRPDFVRK
HHHCEEEECCCCCEEEEEECCCCCEEEHHHHHHHHCCCCHHCCCEEEECCCCHHCCC
>Mature Secondary Structure 
ALNCGSSSVKYQLFDWNQKAVVAKGMVERVTIDDSYIVHEVPGREVYKDEHNCPTHQAA
EECCCCCCEEEEEEECCCCHHHHCCCEEEEEECCCEEEEECCCCHHHCCCCCCCHHHHH
IDLIIRTVTDSEQGVIKNVSEIAAVGHRLVHGGEKFTCSVVIDENVLETVKECQHLAPLH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHCCCC
NPPNIEGVQAARALLPTVPQVAVFDTAFHQTMPEQAYIYPVPYEWYQKHQVRRYGFHGTS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCC
HLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGTRCG
EEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCCCCCCEEECCCC
DIDPAIPLFIMQKEGLNAKEIDTILNKKSGIMGITGRFSDRRDVSHHAETGDKLCKLAMD
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
IEAYRIKKYIGAYMAAVGKLDAVVFTAGVGEMSVETREMVLEGMEHLGLILDKERNKVRT
HHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEECCHHHHHH
RKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYADHMNFEYSFSRPDFVRK
HHHCEEEECCCCCEEEEEECCCCCEEEHHHHHHHHCCCCHHCCCEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA