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The map label for this gene is rfbB [H]

Identifier: 118580514

GI number: 118580514

Start: 2256138

End: 2257208

Strand: Reverse

Name: rfbB [H]

Synonym: Ppro_2098

Alternate gene names: 118580514

Gene position: 2257208-2256138 (Counterclockwise)

Preceding gene: 118580516

Following gene: 118580513

Centisome position: 56.32

GC content: 61.44

Gene sequence:

>1071_bases
ATGCCATGTACTCCCCGTTCGCTTCTTGTGACCGGCGGCGCCGGCTTTATCGGATCAAATTTCATTCATTCATTTCTCGC
CAACAACTCCGACTGCCGGGTGACCAACCTGGATAGCCTGACCTATGCCGGGAATCTGGCCAACCTGAAGAGTCTGGAGC
ATCACCCCGGCTACCATTTCGTCAGGGGCGACATCGGCGACAGCGTACTGGTGGCCAATATCCTGGCGGTTCAGAAGATA
GACGCGGTGGTGCATTTCGCCGCCGAATCCCACGTGGACCGCTCCATCAGCGGCCCGGAGATCTTCGTACGCACCAATGT
GCTGGGAACCCAGGTGCTGCTGGAGGAGAGCCGCAAGCACTGGAACGCGGGGGAGGTCGGTGATTTCCGCTTCCTGCAGA
TTTCCACCGACGAGGTCTACGGCTCCCTGGGGGAGAGCGGCTTCTTCACCGAAGAGACCCCCCTGGCGCCTAATTCACCC
TATTCGGCCAGCAAGGCCGGTGCTGACCTGCTGGTGCGCGCCTACCACGAAACCTTTGGGCTTCCGACCCTGAACACGCG
CTGCTCCAATAACTACGGCCCGTTCCACTTTCCCGAGAAGCTGATCCCGCTTCTGATCCACAACATCATCGAGCGCAGGC
CGTTGCCGGTGTACGGTGATGGCAGAAACGTGCGCGACTGGCTGCACGTCAGCGATCATGCCCGGGCAGTGGAGGTGGTG
CTGAAGGGGGCAGAGCCCGGTGCGGTCTACAACATCGGCGGCAACAACGAGTGGTTCAACATCGCTATCGTCAACCTGGT
GTGCGACCTGATGGACCAGCGGCTGGGGAGGGGTTCCGGCGAGAGCCGGGGGTTGATCAGCTATGTCAAGGACCGCCCCG
GCCATGACCGGCGCTATGCCATCGACGCCTCCAGATTGAAGCGCGACCTGGGGTGGGAACCGGCCTATACCTTTGAACGG
GGCATCGCCGAGACCGTCGACTGGTACCTGGCCAATCAGGAGTGGGTGGCCCAGGTCACCTCCGGCTCCTATCGCGACTA
CTACGCCAAACAGTACGGAGGCGCGCAATGA

Upstream 100 bases:

>100_bases
CGAGATGCCCTGCTTTCCGCTTCTTGAAGGTAAATGAGTTCACATCGTTGGCGAAAATATGCTAGAAAGAAGTGCTTGCT
CCACCCAGAAAGGAACTGCC

Downstream 100 bases:

>100_bases
TTCTTGTCATTGGATGCAACGGCATGCTGGGCAGCGACCTGATGACGCTGCTGGAGGGGAGGGGGCGTGGCGTGGATGTC
CGGGATCTGGATATCACCTC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPCTPRSLLVTGGAGFIGSNFIHSFLANNSDCRVTNLDSLTYAGNLANLKSLEHHPGYHFVRGDIGDSVLVANILAVQKI
DAVVHFAAESHVDRSISGPEIFVRTNVLGTQVLLEESRKHWNAGEVGDFRFLQISTDEVYGSLGESGFFTEETPLAPNSP
YSASKAGADLLVRAYHETFGLPTLNTRCSNNYGPFHFPEKLIPLLIHNIIERRPLPVYGDGRNVRDWLHVSDHARAVEVV
LKGAEPGAVYNIGGNNEWFNIAIVNLVCDLMDQRLGRGSGESRGLISYVKDRPGHDRRYAIDASRLKRDLGWEPAYTFER
GIAETVDWYLANQEWVAQVTSGSYRDYYAKQYGGAQ

Sequences:

>Translated_356_residues
MPCTPRSLLVTGGAGFIGSNFIHSFLANNSDCRVTNLDSLTYAGNLANLKSLEHHPGYHFVRGDIGDSVLVANILAVQKI
DAVVHFAAESHVDRSISGPEIFVRTNVLGTQVLLEESRKHWNAGEVGDFRFLQISTDEVYGSLGESGFFTEETPLAPNSP
YSASKAGADLLVRAYHETFGLPTLNTRCSNNYGPFHFPEKLIPLLIHNIIERRPLPVYGDGRNVRDWLHVSDHARAVEVV
LKGAEPGAVYNIGGNNEWFNIAIVNLVCDLMDQRLGRGSGESRGLISYVKDRPGHDRRYAIDASRLKRDLGWEPAYTFER
GIAETVDWYLANQEWVAQVTSGSYRDYYAKQYGGAQ
>Mature_355_residues
PCTPRSLLVTGGAGFIGSNFIHSFLANNSDCRVTNLDSLTYAGNLANLKSLEHHPGYHFVRGDIGDSVLVANILAVQKID
AVVHFAAESHVDRSISGPEIFVRTNVLGTQVLLEESRKHWNAGEVGDFRFLQISTDEVYGSLGESGFFTEETPLAPNSPY
SASKAGADLLVRAYHETFGLPTLNTRCSNNYGPFHFPEKLIPLLIHNIIERRPLPVYGDGRNVRDWLHVSDHARAVEVVL
KGAEPGAVYNIGGNNEWFNIAIVNLVCDLMDQRLGRGSGESRGLISYVKDRPGHDRRYAIDASRLKRDLGWEPAYTFERG
IAETVDWYLANQEWVAQVTSGSYRDYYAKQYGGAQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=38.7878787878788, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=24.5562130177515, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI4504031, Length=347, Percent_Identity=24.4956772334294, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=52.2099447513812, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=52.4495677233429, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=28.4883720930233, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788366, Length=304, Percent_Identity=27.9605263157895, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788365, Length=283, Percent_Identity=25.4416961130742, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=34.0425531914894, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=33.1378299120235, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=23.3532934131737, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=25.0737463126844, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=367, Percent_Identity=28.0653950953678, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=28.2548476454294, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=22.1212121212121, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24158427, Length=331, Percent_Identity=26.2839879154079, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39473; Mature: 39341

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCTPRSLLVTGGAGFIGSNFIHSFLANNSDCRVTNLDSLTYAGNLANLKSLEHHPGYHF
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHHHHCCCCCEEE
VRGDIGDSVLVANILAVQKIDAVVHFAAESHVDRSISGPEIFVRTNVLGTQVLLEESRKH
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCC
WNAGEVGDFRFLQISTDEVYGSLGESGFFTEETPLAPNSPYSASKAGADLLVRAYHETFG
CCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCC
LPTLNTRCSNNYGPFHFPEKLIPLLIHNIIERRPLPVYGDGRNVRDWLHVSDHARAVEVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCHHHEEHHE
LKGAEPGAVYNIGGNNEWFNIAIVNLVCDLMDQRLGRGSGESRGLISYVKDRPGHDRRYA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEE
IDASRLKRDLGWEPAYTFERGIAETVDWYLANQEWVAQVTSGSYRDYYAKQYGGAQ
ECHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
PCTPRSLLVTGGAGFIGSNFIHSFLANNSDCRVTNLDSLTYAGNLANLKSLEHHPGYHF
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHHHHCCCCCEEE
VRGDIGDSVLVANILAVQKIDAVVHFAAESHVDRSISGPEIFVRTNVLGTQVLLEESRKH
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHCC
WNAGEVGDFRFLQISTDEVYGSLGESGFFTEETPLAPNSPYSASKAGADLLVRAYHETFG
CCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCC
LPTLNTRCSNNYGPFHFPEKLIPLLIHNIIERRPLPVYGDGRNVRDWLHVSDHARAVEVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCHHHEEHHE
LKGAEPGAVYNIGGNNEWFNIAIVNLVCDLMDQRLGRGSGESRGLISYVKDRPGHDRRYA
EECCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEE
IDASRLKRDLGWEPAYTFERGIAETVDWYLANQEWVAQVTSGSYRDYYAKQYGGAQ
ECHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]