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The map label for this gene is spsK [H]

Identifier: 118580513

GI number: 118580513

Start: 2255299

End: 2256141

Strand: Reverse

Name: spsK [H]

Synonym: Ppro_2097

Alternate gene names: 118580513

Gene position: 2256141-2255299 (Counterclockwise)

Preceding gene: 118580514

Following gene: 118580512

Centisome position: 56.29

GC content: 62.28

Gene sequence:

>843_bases
ATGATTCTTGTCATTGGATGCAACGGCATGCTGGGCAGCGACCTGATGACGCTGCTGGAGGGGAGGGGGCGTGGCGTGGA
TGTCCGGGATCTGGATATCACCTCTCTGGAATCCACCGAACGGTTGCTGACCTCTCTGAAACCTTCCGTGGTGATCAACT
GTGCCGCCTATACGGATGTGGACGGCTGCGAGAGCAACCGGGAGACCGCCATGCTGGTCAACGCAGAGGGTGTTGCCAAC
CTGGCCAGGGTGACCCGCGGCATCGGGGCCAGGCTGGTGCAGATCAGCACCGACTATGTCTTCGACGGCGCCAAGGGAAG
CCCGTACCTGGAGGACGACCAGACCGCTCCCTTAAGCGTGTATGGCCAGTCCAAGCTGCTGGGGGAGAGAAACGCCGCCA
CCAACCCGGATCACCTGATCGTGCGCACCCAGTGGCTCTATGGCCTGCAGGGAAATAACTTCGTCGAAACCATGCTGCGT
CTAGGAACGGAGAGGGAGTGCCTGACGGTGGTGGACGACCAGATCGGCTCTCCCACCTGGACTGTGGACCTCTCCCGTGC
CATCCTGGCGCTTCTGGCCAGGGGCTGCCGCGGCACCTATCACGCCGCCAACGCCGGCTTTTGCTCCTGGAACGAGTTTG
CCCGCGCCATCTTCCGGGAGGCCGGTCTGGGAGTGAGGGTCGAGCCCATGACCACGGAGCAGCTGAACCGTCCGGCCAAG
CGGCCGCTCTATTCCACGCTGGATTGCTCCAAACTGGAGCGGGACACCGGTTTCCGTCTCCAGTCCTGGGGTGATGCGCT
GACCGACTACATGAAACTGAGAACACATTCCAACAGGGGGTGA

Upstream 100 bases:

>100_bases
CCGAGACCGTCGACTGGTACCTGGCCAATCAGGAGTGGGTGGCCCAGGTCACCTCCGGCTCCTATCGCGACTACTACGCC
AAACAGTACGGAGGCGCGCA

Downstream 100 bases:

>100_bases
AACATGGAACGGGGAGAACGCCCCTGGGGCTCGTATTCCGTGCTGGATGAGAATAACGGCTACAAGATCAAACGCATCGA
GGTTGCGCCGGGGGAACGGC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILVIGCNGMLGSDLMTLLEGRGRGVDVRDLDITSLESTERLLTSLKPSVVINCAAYTDVDGCESNRETAMLVNAEGVAN
LARVTRGIGARLVQISTDYVFDGAKGSPYLEDDQTAPLSVYGQSKLLGERNAATNPDHLIVRTQWLYGLQGNNFVETMLR
LGTERECLTVVDDQIGSPTWTVDLSRAILALLARGCRGTYHAANAGFCSWNEFARAIFREAGLGVRVEPMTTEQLNRPAK
RPLYSTLDCSKLERDTGFRLQSWGDALTDYMKLRTHSNRG

Sequences:

>Translated_280_residues
MILVIGCNGMLGSDLMTLLEGRGRGVDVRDLDITSLESTERLLTSLKPSVVINCAAYTDVDGCESNRETAMLVNAEGVAN
LARVTRGIGARLVQISTDYVFDGAKGSPYLEDDQTAPLSVYGQSKLLGERNAATNPDHLIVRTQWLYGLQGNNFVETMLR
LGTERECLTVVDDQIGSPTWTVDLSRAILALLARGCRGTYHAANAGFCSWNEFARAIFREAGLGVRVEPMTTEQLNRPAK
RPLYSTLDCSKLERDTGFRLQSWGDALTDYMKLRTHSNRG
>Mature_280_residues
MILVIGCNGMLGSDLMTLLEGRGRGVDVRDLDITSLESTERLLTSLKPSVVINCAAYTDVDGCESNRETAMLVNAEGVAN
LARVTRGIGARLVQISTDYVFDGAKGSPYLEDDQTAPLSVYGQSKLLGERNAATNPDHLIVRTQWLYGLQGNNFVETMLR
LGTERECLTVVDDQIGSPTWTVDLSRAILALLARGCRGTYHAANAGFCSWNEFARAIFREAGLGVRVEPMTTEQLNRPAK
RPLYSTLDCSKLERDTGFRLQSWGDALTDYMKLRTHSNRG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=295, Percent_Identity=27.7966101694915, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI11034825, Length=295, Percent_Identity=27.7966101694915, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788352, Length=280, Percent_Identity=37.1428571428571, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30772; Mature: 30772

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVIGCNGMLGSDLMTLLEGRGRGVDVRDLDITSLESTERLLTSLKPSVVINCAAYTDV
CEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCC
DGCESNRETAMLVNAEGVANLARVTRGIGARLVQISTDYVFDGAKGSPYLEDDQTAPLSV
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEE
YGQSKLLGERNAATNPDHLIVRTQWLYGLQGNNFVETMLRLGTERECLTVVDDQIGSPTW
ECCHHHCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEE
TVDLSRAILALLARGCRGTYHAANAGFCSWNEFARAIFREAGLGVRVEPMTTEQLNRPAK
EEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHCCCCC
RPLYSTLDCSKLERDTGFRLQSWGDALTDYMKLRTHSNRG
CCCHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MILVIGCNGMLGSDLMTLLEGRGRGVDVRDLDITSLESTERLLTSLKPSVVINCAAYTDV
CEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCC
DGCESNRETAMLVNAEGVANLARVTRGIGARLVQISTDYVFDGAKGSPYLEDDQTAPLSV
CCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEE
YGQSKLLGERNAATNPDHLIVRTQWLYGLQGNNFVETMLRLGTERECLTVVDDQIGSPTW
ECCHHHCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEE
TVDLSRAILALLARGCRGTYHAANAGFCSWNEFARAIFREAGLGVRVEPMTTEQLNRPAK
EEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHCCCCC
RPLYSTLDCSKLERDTGFRLQSWGDALTDYMKLRTHSNRG
CCCHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]