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The map label for this gene is btuB [H]

Identifier: 118580493

GI number: 118580493

Start: 2231049

End: 2233277

Strand: Direct

Name: btuB [H]

Synonym: Ppro_2077

Alternate gene names: 118580493

Gene position: 2231049-2233277 (Clockwise)

Preceding gene: 118580492

Following gene: 118580494

Centisome position: 55.66

GC content: 60.3

Gene sequence:

>2229_bases
ATGAGACGACGTATCGCAGTGATACTGGCGCTGGTGACAACCGCAGCGCCGCTGCAAGCGCGGGGAGATGGACAGGATAA
CAGCAAGGAAACAAGCCTGGACGAGGTGGTGGTCACCGCCACCCGCACGGAGAAGTCCATCGCCGATGCCCCCGGTTCGG
TGAGCGTGGTAACCGCAGCGGAGATGAAGAAACGGAACATCCAGTCGGTGGACGAGGCGCTGAACATGCTGCCCGGCGTC
TTTGACAGCCGCCGCAAGGGGTTGATGGGAACCACCTCCAGCGTCACCTTCCGCGGCCTGTCCGGCGCCGGTCGATCCCT
GCTGCTGCTGGACGGCATGCCGCTCAACGACGCCTACTCTGCCAGCCAACCATGGGGTGGCCTGAATGCCCAGAATTTTT
CACAAATAGAGGTTGTGCGCGGACCGTCGTCCAGCCTCTACGGCGGCAACGCCATGGGTGGGGTGATCAACTTCCTGAGC
CACATGCCGGAAAAACGAGAGTTCACCCTCTCCAGCGGCTACGGCGGCGGCCTTGAGACCGACGCGGCCATGGACCGCCT
CTGGAGCAGCTACACCTCCTATGGAGACAGGATCGGCAAACTGCGGCTGTATGCCGCCCTATCCTACCGCAGCACCGACG
GCTACCCCAGCGGGCTGGTCACCACGACCACCAACCCCTCGGCCTCGGGGATCAGCGGCGCGCACCCCACAACGGACACC
ACGGGAAAAGCGACAAAATACATCACGGGCGACAGCGGGGACAACGGCTGGTGGGACTACTCGGCGGCCATCAGGGCTCA
CTACGATTTTTCAGATACCGCCAACATCAGGATCTCCTGGCTCAGGACCGCCAACAAGTATTCCTACGACCCTCCCCACA
CCTATCTGAGAGACGCCTCCGGGGCAGAGGCATACAGGCCCGGCCAAAGCAGCTACCTGAACGGAGACGGCGCCAATGTC
CAGGACATGTACACCATTCACGCCGAAGCGCTGCTGGGTCAGGTGAAGGGCAAATTCCTCTTCGGCATCAACGACCAGCA
TGAAAACTGGTATACCACCCCCGGCAGTTCGGCGACTGTGGCCAACGGCGGAACCGGCACAAAAAGCGACTCTCCCAGCA
GGACCTATTTCACCGACCTGCAACTGTCCGTCCCGGTCCTGGAAAAACACATCCTCACCGGCGGTTTCTCTTTCCGCTAC
GACGTAGCCGACAGCGATAACCTCCCCTTGAGCGATTACGAGGACGAAGACTCTGTTTCCGGCCCCAGCACCCAGTGGGG
GGGGGGCACAACCCAGACCTACTCCACCTACCTGCAGGCCGAAATAGCCCTGCTGGACAACCTGACCTTCTACGCTGGCG
TGCGGGACGATTACTGGGTCTCCAACAACGGCTACGCCGGATTCAGCGGCACCGGAGCCAGCGATCAGAAGTATCCCGAG
AAATCCAAGAACTCCATCAACCCCAAGGGCGCGCTGGTCTGGAAGCCCCTGGACGGAACCACGGTGAGGATTTCGGGCGG
CAGGGCCTTCCGCGCACCGCAGTTGAACCAGATGTACAAGACCTGGTCATCGTCCAGCACCTCCAAGCTGTACCTAGCCA
ATCCCGACCTGAATCCCGAAACCGTTCTGGCCTGGGATTTCGGCATCGAACAGAAACTGTGGAAGGGCGCCAAGATCAAG
GCAACCTACTTCGAGAACTACATCGACGACATGATCTACACCGTCACCCTTTCCAGCACGACCAGCATCGGCGGCAAGAA
CTACACCAACGTGAAATATTCCAATCTTGGGGCAGGTGAGACCAGGGGGGTGGAACTGGAAGCGGAGCAGAGCCTGGGCA
AAAATCTGCGCCTGTTTGCCGGCTACACCTACACCAGCTCCACTATCACCGAGTACGATGAGAACAAAAGCCTGGAGGGC
AAGGATCTGCAGCATGCGCCCCGCCACATGATCAACGCGGGGCTGGACGCCAGCTACGGCCCGCTGTCGTTCTTTCTGTC
CGGAAGATACGTGGCCAAGCAGTACGGTTCGGATAACAACAGCGACAAGGCCAACGGGGTCTACGGCTCCTACGATCCCT
TCTTCGTCATGGACCTGAAGGCCGGATACCGGGTTACTGACTGGGCTACGCTCTCCTTGAGCGTGAACAACCTGCTGGAC
CGCACCTATTACACCTACTACGTCGCACCGGGCCGCTCCTGGTTCGCCAATCTCGACCTTAGGTTCTGA

Upstream 100 bases:

>100_bases
AGTGCGTATGACAGAAATAAGCGAAGAGCATCAATATAACGAGCTCTTATACTCTGACATGACAACAGATGTGATGCAAT
TTCATTGCAAGGAGAGAGGG

Downstream 100 bases:

>100_bases
GCCATGAAAAGCCGTCTTTGTCATGCGCTCCTTTCTCTGTTGATCCTGTTGCCGGCGTTCGCCACGCCGGCTGCGGCATT
CACCTACCGCGACAAGATGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MRRRIAVILALVTTAAPLQARGDGQDNSKETSLDEVVVTATRTEKSIADAPGSVSVVTAAEMKKRNIQSVDEALNMLPGV
FDSRRKGLMGTTSSVTFRGLSGAGRSLLLLDGMPLNDAYSASQPWGGLNAQNFSQIEVVRGPSSSLYGGNAMGGVINFLS
HMPEKREFTLSSGYGGGLETDAAMDRLWSSYTSYGDRIGKLRLYAALSYRSTDGYPSGLVTTTTNPSASGISGAHPTTDT
TGKATKYITGDSGDNGWWDYSAAIRAHYDFSDTANIRISWLRTANKYSYDPPHTYLRDASGAEAYRPGQSSYLNGDGANV
QDMYTIHAEALLGQVKGKFLFGINDQHENWYTTPGSSATVANGGTGTKSDSPSRTYFTDLQLSVPVLEKHILTGGFSFRY
DVADSDNLPLSDYEDEDSVSGPSTQWGGGTTQTYSTYLQAEIALLDNLTFYAGVRDDYWVSNNGYAGFSGTGASDQKYPE
KSKNSINPKGALVWKPLDGTTVRISGGRAFRAPQLNQMYKTWSSSSTSKLYLANPDLNPETVLAWDFGIEQKLWKGAKIK
ATYFENYIDDMIYTVTLSSTTSIGGKNYTNVKYSNLGAGETRGVELEAEQSLGKNLRLFAGYTYTSSTITEYDENKSLEG
KDLQHAPRHMINAGLDASYGPLSFFLSGRYVAKQYGSDNNSDKANGVYGSYDPFFVMDLKAGYRVTDWATLSLSVNNLLD
RTYYTYYVAPGRSWFANLDLRF

Sequences:

>Translated_742_residues
MRRRIAVILALVTTAAPLQARGDGQDNSKETSLDEVVVTATRTEKSIADAPGSVSVVTAAEMKKRNIQSVDEALNMLPGV
FDSRRKGLMGTTSSVTFRGLSGAGRSLLLLDGMPLNDAYSASQPWGGLNAQNFSQIEVVRGPSSSLYGGNAMGGVINFLS
HMPEKREFTLSSGYGGGLETDAAMDRLWSSYTSYGDRIGKLRLYAALSYRSTDGYPSGLVTTTTNPSASGISGAHPTTDT
TGKATKYITGDSGDNGWWDYSAAIRAHYDFSDTANIRISWLRTANKYSYDPPHTYLRDASGAEAYRPGQSSYLNGDGANV
QDMYTIHAEALLGQVKGKFLFGINDQHENWYTTPGSSATVANGGTGTKSDSPSRTYFTDLQLSVPVLEKHILTGGFSFRY
DVADSDNLPLSDYEDEDSVSGPSTQWGGGTTQTYSTYLQAEIALLDNLTFYAGVRDDYWVSNNGYAGFSGTGASDQKYPE
KSKNSINPKGALVWKPLDGTTVRISGGRAFRAPQLNQMYKTWSSSSTSKLYLANPDLNPETVLAWDFGIEQKLWKGAKIK
ATYFENYIDDMIYTVTLSSTTSIGGKNYTNVKYSNLGAGETRGVELEAEQSLGKNLRLFAGYTYTSSTITEYDENKSLEG
KDLQHAPRHMINAGLDASYGPLSFFLSGRYVAKQYGSDNNSDKANGVYGSYDPFFVMDLKAGYRVTDWATLSLSVNNLLD
RTYYTYYVAPGRSWFANLDLRF
>Mature_742_residues
MRRRIAVILALVTTAAPLQARGDGQDNSKETSLDEVVVTATRTEKSIADAPGSVSVVTAAEMKKRNIQSVDEALNMLPGV
FDSRRKGLMGTTSSVTFRGLSGAGRSLLLLDGMPLNDAYSASQPWGGLNAQNFSQIEVVRGPSSSLYGGNAMGGVINFLS
HMPEKREFTLSSGYGGGLETDAAMDRLWSSYTSYGDRIGKLRLYAALSYRSTDGYPSGLVTTTTNPSASGISGAHPTTDT
TGKATKYITGDSGDNGWWDYSAAIRAHYDFSDTANIRISWLRTANKYSYDPPHTYLRDASGAEAYRPGQSSYLNGDGANV
QDMYTIHAEALLGQVKGKFLFGINDQHENWYTTPGSSATVANGGTGTKSDSPSRTYFTDLQLSVPVLEKHILTGGFSFRY
DVADSDNLPLSDYEDEDSVSGPSTQWGGGTTQTYSTYLQAEIALLDNLTFYAGVRDDYWVSNNGYAGFSGTGASDQKYPE
KSKNSINPKGALVWKPLDGTTVRISGGRAFRAPQLNQMYKTWSSSSTSKLYLANPDLNPETVLAWDFGIEQKLWKGAKIK
ATYFENYIDDMIYTVTLSSTTSIGGKNYTNVKYSNLGAGETRGVELEAEQSLGKNLRLFAGYTYTSSTITEYDENKSLEG
KDLQHAPRHMINAGLDASYGPLSFFLSGRYVAKQYGSDNNSDKANGVYGSYDPFFVMDLKAGYRVTDWATLSLSVNNLLD
RTYYTYYVAPGRSWFANLDLRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=167, Percent_Identity=32.9341317365269, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790405, Length=149, Percent_Identity=34.8993288590604, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786344, Length=131, Percent_Identity=35.8778625954198, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786798, Length=173, Percent_Identity=29.4797687861272, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 80946; Mature: 80946

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRIAVILALVTTAAPLQARGDGQDNSKETSLDEVVVTATRTEKSIADAPGSVSVVTAA
CCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEEECCCHHHHCCCCCEEEEEHH
EMKKRNIQSVDEALNMLPGVFDSRRKGLMGTTSSVTFRGLSGAGRSLLLLDGMPLNDAYS
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
ASQPWGGLNAQNFSQIEVVRGPSSSLYGGNAMGGVINFLSHMPEKREFTLSSGYGGGLET
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
DAAMDRLWSSYTSYGDRIGKLRLYAALSYRSTDGYPSGLVTTTTNPSASGISGAHPTTDT
HHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
TGKATKYITGDSGDNGWWDYSAAIRAHYDFSDTANIRISWLRTANKYSYDPPHTYLRDAS
CCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHCCCC
GAEAYRPGQSSYLNGDGANVQDMYTIHAEALLGQVKGKFLFGINDQHENWYTTPGSSATV
CCCCCCCCCCCEECCCCCCCHHEEEHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCEEE
ANGGTGTKSDSPSRTYFTDLQLSVPVLEKHILTGGFSFRYDVADSDNLPLSDYEDEDSVS
ECCCCCCCCCCCCCEEEEEEEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
GPSTQWGGGTTQTYSTYLQAEIALLDNLTFYAGVRDDYWVSNNGYAGFSGTGASDQKYPE
CCCCCCCCCCCHHHHHHHHEEEEHHCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCC
KSKNSINPKGALVWKPLDGTTVRISGGRAFRAPQLNQMYKTWSSSSTSKLYLANPDLNPE
CCCCCCCCCCEEEEECCCCCEEEEECCCEECCCCHHHHHHHHCCCCCCEEEEECCCCCCC
TVLAWDFGIEQKLWKGAKIKATYFENYIDDMIYTVTLSSTTSIGGKNYTNVKYSNLGAGE
EEEEEECCCHHHHCCCCEEEHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEECCCCCCC
TRGVELEAEQSLGKNLRLFAGYTYTSSTITEYDENKSLEGKDLQHAPRHMINAGLDASYG
CCCEEEEEHHHCCCCEEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PLSFFLSGRYVAKQYGSDNNSDKANGVYGSYDPFFVMDLKAGYRVTDWATLSLSVNNLLD
CEEEEECCCEEEHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECEEEEEEEHHHHHC
RTYYTYYVAPGRSWFANLDLRF
CEEEEEEEECCCCEEEEEEECC
>Mature Secondary Structure
MRRRIAVILALVTTAAPLQARGDGQDNSKETSLDEVVVTATRTEKSIADAPGSVSVVTAA
CCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEEECCCHHHHCCCCCEEEEEHH
EMKKRNIQSVDEALNMLPGVFDSRRKGLMGTTSSVTFRGLSGAGRSLLLLDGMPLNDAYS
HHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
ASQPWGGLNAQNFSQIEVVRGPSSSLYGGNAMGGVINFLSHMPEKREFTLSSGYGGGLET
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
DAAMDRLWSSYTSYGDRIGKLRLYAALSYRSTDGYPSGLVTTTTNPSASGISGAHPTTDT
HHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
TGKATKYITGDSGDNGWWDYSAAIRAHYDFSDTANIRISWLRTANKYSYDPPHTYLRDAS
CCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHCCCC
GAEAYRPGQSSYLNGDGANVQDMYTIHAEALLGQVKGKFLFGINDQHENWYTTPGSSATV
CCCCCCCCCCCEECCCCCCCHHEEEHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCEEE
ANGGTGTKSDSPSRTYFTDLQLSVPVLEKHILTGGFSFRYDVADSDNLPLSDYEDEDSVS
ECCCCCCCCCCCCCEEEEEEEEECHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCC
GPSTQWGGGTTQTYSTYLQAEIALLDNLTFYAGVRDDYWVSNNGYAGFSGTGASDQKYPE
CCCCCCCCCCCHHHHHHHHEEEEHHCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCC
KSKNSINPKGALVWKPLDGTTVRISGGRAFRAPQLNQMYKTWSSSSTSKLYLANPDLNPE
CCCCCCCCCCEEEEECCCCCEEEEECCCEECCCCHHHHHHHHCCCCCCEEEEECCCCCCC
TVLAWDFGIEQKLWKGAKIKATYFENYIDDMIYTVTLSSTTSIGGKNYTNVKYSNLGAGE
EEEEEECCCHHHHCCCCEEEHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEECCCCCCC
TRGVELEAEQSLGKNLRLFAGYTYTSSTITEYDENKSLEGKDLQHAPRHMINAGLDASYG
CCCEEEEEHHHCCCCEEEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCC
PLSFFLSGRYVAKQYGSDNNSDKANGVYGSYDPFFVMDLKAGYRVTDWATLSLSVNNLLD
CEEEEECCCEEEHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECEEEEEEEHHHHHC
RTYYTYYVAPGRSWFANLDLRF
CEEEEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA