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The map label for this gene is guaA

Identifier: 118580426

GI number: 118580426

Start: 2157679

End: 2159241

Strand: Reverse

Name: guaA

Synonym: Ppro_2010

Alternate gene names: 118580426

Gene position: 2159241-2157679 (Counterclockwise)

Preceding gene: 118580427

Following gene: 118580425

Centisome position: 53.87

GC content: 64.17

Gene sequence:

>1563_bases
ATGAAAACTGACATCCACAGCCAGAAGATCCTGATCCTCGATTTCGGCTCCCAGTACACCCAGCTGATCGCCCGGCGGGT
GCGCGAGGCCCACGTGTTCTGCGAACTGCACCCCTTTGACATGGAGATGGCGGCCATCCGCGCCTTCAATCCCTCGGGCA
TCATCCTGTCCGGCGGCCCCAAATCGGTCTACGAGCCGGGAGCGCCGGCAGTTGCCGAGGAGCTGTTCGAGCTGGGAGTT
CCGGTGCTGGGGATCTGCTACGGCATGCAGCTCATGAGCCGCCACTTCGGCGGAGAAGTGGTGCCTGCCGGCAAGCGCGA
GTTCGGCCACGCCGAGTTGCTTTGTTCCGGCCAGCCCGGCCAGCTGTTCGACGGCTTCTTCGTGGGGGGGGCGAGCCCGG
TCTGGATGAGTCATGGCGACCACGTCTCCCGCGTGCCGGACGGATTCCAGGTGGTGGCCTACACGGAGAACGCCCCGGTC
TGCGCCATCCAGGATGTGAGCCGCAACCTGTACGGGGTGCAGTTCCATCCCGAGGTCAACCACACCCCCCGCGGCGACCA
GCTGATCGACACCTTTGTGCGTGCCATCTGCGGCTGCACCGGTCACTGGACACCCGGCCAGATCATCGAGGATGCCGTTA
CCCGCATCCAAGAACAGGTGGGCAGCGAACGGGTGATCCTGGGGCTTTCCGGCGGGGTGGACTCGTCGGTGGCTGCCGCA
TTGATCCATCGGGCCATTGGCGACCAGCTGACCTGCGTCTTCGTGGACAACGGCCTGCTGCGCCTGGAGGAGGGTGACCA
GGTGATGCGCACCTTTGCCGAAAACCTGGGGGTCAAGGTGATCCGGGTCAACGCCGAGGAGCGTTTCCTAAGCGCCCTGG
CCGGGGAGAGCGATCCGGAGAAAAAGCGCAAGATCATCGGCAGCCTGTTCGTGGAGATCTTCGACGAGGAGTCCAACCGC
ATCGAGGACGCCCGCTGGCTGGCCCAGGGGACCATCTACCCGGACGTTATCGAGTCGGCCGGGGCCAAGACCGGCAAGGC
CCACAACATCAAGAGCCACCACAACGTGGGCGGGCTTCCGGAGTACATGAAGCTCAAGCTCCTGGAACCGCTGCGCGAAC
TGTTCAAGGACGAGGTGCGCGCCATCGGCGAGGAGCTGGGGCTCCCCCATCAGATGGTCTGGCGCCACCCTTTCCCCGGG
CCGGGACTGGCCGTGCGCATCCTGGGAGAGGTGAAGAAGGAGTACGCCGACATCCTTCGCAGGGCGGATGCCATCTTCAT
CGAGGAGCTCTACTCCTCCGACCATTACCACAAGATCAGCCAGGCTTTTGCCGTGTTCCTGCCGGTGAAGAGCGTGGGGG
TCATGGGCGACGGCCGCACCTACGAGTATGTGGTGGCCCTGCGGGCGGTGGAAACCAAAGACTTCATGACCGCCAGCTGG
TACCCGCTCCCCTACGAAGACATGGCCCGCATCAGTGCTAGGATCATCAACGAGGTCAAGGGGGTCAACCGGGTGGTGTA
CGACATCTCCAGCAAGCCGCCCGCCACCATCGAGTGGGAGTGA

Upstream 100 bases:

>100_bases
GCGCTTCATACGCATCACCGGCGCCGGCCTCAAGGAATCCCACGTGCATGACGTCACCATCACCAAGGAAGCGCCCAACT
ACAGGGTGGGAAACTGACCC

Downstream 100 bases:

>100_bases
TTCAGGGCGTATCCAGCGCCATTGGGCTGACTGTATCTATGAATGGCGCCGCGGATCTGTGAAAAACGCGCCGTACGCTA
GAATTGGCGGATCATGTATC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKTDIHSQKILILDFGSQYTQLIARRVREAHVFCELHPFDMEMAAIRAFNPSGIILSGGPKSVYEPGAPAVAEELFELGV
PVLGICYGMQLMSRHFGGEVVPAGKREFGHAELLCSGQPGQLFDGFFVGGASPVWMSHGDHVSRVPDGFQVVAYTENAPV
CAIQDVSRNLYGVQFHPEVNHTPRGDQLIDTFVRAICGCTGHWTPGQIIEDAVTRIQEQVGSERVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDNGLLRLEEGDQVMRTFAENLGVKVIRVNAEERFLSALAGESDPEKKRKIIGSLFVEIFDEESNR
IEDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPEYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPG
PGLAVRILGEVKKEYADILRRADAIFIEELYSSDHYHKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTASW
YPLPYEDMARISARIINEVKGVNRVVYDISSKPPATIEWE

Sequences:

>Translated_520_residues
MKTDIHSQKILILDFGSQYTQLIARRVREAHVFCELHPFDMEMAAIRAFNPSGIILSGGPKSVYEPGAPAVAEELFELGV
PVLGICYGMQLMSRHFGGEVVPAGKREFGHAELLCSGQPGQLFDGFFVGGASPVWMSHGDHVSRVPDGFQVVAYTENAPV
CAIQDVSRNLYGVQFHPEVNHTPRGDQLIDTFVRAICGCTGHWTPGQIIEDAVTRIQEQVGSERVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDNGLLRLEEGDQVMRTFAENLGVKVIRVNAEERFLSALAGESDPEKKRKIIGSLFVEIFDEESNR
IEDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPEYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPG
PGLAVRILGEVKKEYADILRRADAIFIEELYSSDHYHKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTASW
YPLPYEDMARISARIINEVKGVNRVVYDISSKPPATIEWE
>Mature_520_residues
MKTDIHSQKILILDFGSQYTQLIARRVREAHVFCELHPFDMEMAAIRAFNPSGIILSGGPKSVYEPGAPAVAEELFELGV
PVLGICYGMQLMSRHFGGEVVPAGKREFGHAELLCSGQPGQLFDGFFVGGASPVWMSHGDHVSRVPDGFQVVAYTENAPV
CAIQDVSRNLYGVQFHPEVNHTPRGDQLIDTFVRAICGCTGHWTPGQIIEDAVTRIQEQVGSERVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDNGLLRLEEGDQVMRTFAENLGVKVIRVNAEERFLSALAGESDPEKKRKIIGSLFVEIFDEESNR
IEDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPEYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPG
PGLAVRILGEVKKEYADILRRADAIFIEELYSSDHYHKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTASW
YPLPYEDMARISARIINEVKGVNRVVYDISSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=558, Percent_Identity=38.3512544802867, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=63.0476190476191, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1789760, Length=157, Percent_Identity=31.2101910828025, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71992710, Length=572, Percent_Identity=38.2867132867133, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI133901714, Length=572, Percent_Identity=38.2867132867133, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71992717, Length=572, Percent_Identity=38.2867132867133, Blast_Score=325, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6323873, Length=517, Percent_Identity=51.6441005802708, Blast_Score=549, Evalue=1e-157,
Organism=Drosophila melanogaster, GI281365319, Length=553, Percent_Identity=37.251356238698, Blast_Score=318, Evalue=5e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_PELPD (A1AQJ8)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901676.1
- ProteinModelPortal:   A1AQJ8
- SMR:   A1AQJ8
- STRING:   A1AQJ8
- GeneID:   4572681
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_2010
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- PhylomeDB:   A1AQJ8
- ProtClustDB:   PRK00074
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57595; Mature: 57595

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 86-86 ACT_SITE 176-176 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDIHSQKILILDFGSQYTQLIARRVREAHVFCELHPFDMEMAAIRAFNPSGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHEEECCCCEEEECCC
KSVYEPGAPAVAEELFELGVPVLGICYGMQLMSRHFGGEVVPAGKREFGHAELLCSGQPG
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
QLFDGFFVGGASPVWMSHGDHVSRVPDGFQVVAYTENAPVCAIQDVSRNLYGVQFHPEVN
CCCCCEEECCCCCEEECCCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCEEEEEECCCCC
HTPRGDQLIDTFVRAICGCTGHWTPGQIIEDAVTRIQEQVGSERVILGLSGGVDSSVAAA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
LIHRAIGDQLTCVFVDNGLLRLEEGDQVMRTFAENLGVKVIRVNAEERFLSALAGESDPE
HHHHHHCCCEEEEEECCCEEEECCCHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCHH
KKRKIIGSLFVEIFDEESNRIEDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCH
EYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPGPGLAVRILGEVKKEYADILR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
RADAIFIEELYSSDHYHKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTASW
HHHHHHHHHHHCCCCHHHHHHHHHHHEEHHHHCCCCCCCCEEEEEEEEHHCCCCCEEECC
YPLPYEDMARISARIINEVKGVNRVVYDISSKPPATIEWE
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECC
>Mature Secondary Structure
MKTDIHSQKILILDFGSQYTQLIARRVREAHVFCELHPFDMEMAAIRAFNPSGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHEEECCCCEEEECCC
KSVYEPGAPAVAEELFELGVPVLGICYGMQLMSRHFGGEVVPAGKREFGHAELLCSGQPG
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
QLFDGFFVGGASPVWMSHGDHVSRVPDGFQVVAYTENAPVCAIQDVSRNLYGVQFHPEVN
CCCCCEEECCCCCEEECCCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCEEEEEECCCCC
HTPRGDQLIDTFVRAICGCTGHWTPGQIIEDAVTRIQEQVGSERVILGLSGGVDSSVAAA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
LIHRAIGDQLTCVFVDNGLLRLEEGDQVMRTFAENLGVKVIRVNAEERFLSALAGESDPE
HHHHHHCCCEEEEEECCCEEEECCCHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCHH
KKRKIIGSLFVEIFDEESNRIEDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLP
HHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCH
EYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPGPGLAVRILGEVKKEYADILR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
RADAIFIEELYSSDHYHKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTASW
HHHHHHHHHHHCCCCHHHHHHHHHHHEEHHHHCCCCCCCCEEEEEEEEHHCCCCCEEECC
YPLPYEDMARISARIINEVKGVNRVVYDISSKPPATIEWE
CCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA