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The map label for this gene is livJ [H]

Identifier: 118580387

GI number: 118580387

Start: 2113533

End: 2114651

Strand: Direct

Name: livJ [H]

Synonym: Ppro_1970

Alternate gene names: 118580387

Gene position: 2113533-2114651 (Clockwise)

Preceding gene: 118580386

Following gene: 118580388

Centisome position: 52.73

GC content: 62.73

Gene sequence:

>1119_bases
ATGCCCCTCCGTATTCGTTTCTTATACTCTCTTCTCGCACTGGGCTGCATCGCCCTGTTTCTGAGCGGGTGCGAACGGAA
GCCGATCCGCATCGGGTTTGTGGGCGGCATGTCGGGGCGCGTGGCGGACCTTGGGGTCGGCGGCAGAAACGGCGCCCTGC
TTGCCGTAGAGGAGAGAAACGCCCGTGGCGGCATCAACGGGCGGCCGGTTGAGCTGATCATCCGCGATGACGAGCAAAAC
GTGGAGACCGCCAGAAAGGTTACCCGCGAACTGACGGAGAGCAACGTGGAGGCGCTCATCGGCCCCATGACCAGCAGCGT
GGCGCTGGCCATGGTTCCCATCGTCAATCAGGCGCGACTGGTCACGGTCAGCCCCACCGTGACCAGCACGCAACTCTCCG
GGGCGGACGATTACTTCCTGCGCGTTCTCCCGGACACCTCCACCTACGCTCCCAAGAGCGCCCTTTTTCACCTGAAAAAG
TCCGGCCTGCGCCGGGCCGCCATTGTGTACGACACGGGCAACAGCTCCTACACGACCAGCTGGCTGGCCGGTTTCAGGAA
GAGCTTTGAGAACAGCGGCGGCAGCATCGTCGTCAGCAGATCATTCACCTCCGGTGAAAACATCGCCTTTTCTCCCATCG
TGCGCGACCTGCTCCGCCACGACCCCGATCTGGTGGTCCTGGTCTGCAACGCCGTCGATGCCGCCCTGCTCTGCCAGCAG
ATTCGCAAACTGGACCGCCGCGTCACCATCACTGTATCCGAGTGGGGCTCCACGGAGCGCTTCATCGAGCTGGGAGGCTC
CGCCGTAGAGGGCGTGTACTTCGCGCAGTTCATCAACCACGGCGACGTGACGCCCCGCTACCGGAACTTCCTGGCTTCAT
ACCGGACCCGTTTCGGGCAGGATCCCGGCTTCCCGGGGCTGGCCGGCTATGACGCGGCAAACGTCGTGCTGGACGCCCTG
ACCGCACGGAAGGGAGACGCTTCCCTTAAAGACACAATCGTCTCTGCCGGCAGCTATCAGGGGGTCCAGAGGAAGATCGT
CCTGGATCGTTTTGGCGACGCCAGGAGCACGACCTTCATCACCGTGGTTAAAGACGGTCGTTTCGTAACCCTCGAGTAA

Upstream 100 bases:

>100_bases
GCGCCCGTTGTTATAGATCGTATACTCTTCACGAAAGCAGCCACCACACAATGGCCCCGACTCCGATTCCCCCTTTGGGC
CGTGAAAGGTACGACCTGCC

Downstream 100 bases:

>100_bases
TCAGCAATGAAAATCCGCTCACTCCGTCATCTTCTTACCATCCGCTCAGCCTGGGTAGCCGTGGTTCCCTTCCTCTTCTC
GGCGGTTCTGGGGTGGTTCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MPLRIRFLYSLLALGCIALFLSGCERKPIRIGFVGGMSGRVADLGVGGRNGALLAVEERNARGGINGRPVELIIRDDEQN
VETARKVTRELTESNVEALIGPMTSSVALAMVPIVNQARLVTVSPTVTSTQLSGADDYFLRVLPDTSTYAPKSALFHLKK
SGLRRAAIVYDTGNSSYTTSWLAGFRKSFENSGGSIVVSRSFTSGENIAFSPIVRDLLRHDPDLVVLVCNAVDAALLCQQ
IRKLDRRVTITVSEWGSTERFIELGGSAVEGVYFAQFINHGDVTPRYRNFLASYRTRFGQDPGFPGLAGYDAANVVLDAL
TARKGDASLKDTIVSAGSYQGVQRKIVLDRFGDARSTTFITVVKDGRFVTLE

Sequences:

>Translated_372_residues
MPLRIRFLYSLLALGCIALFLSGCERKPIRIGFVGGMSGRVADLGVGGRNGALLAVEERNARGGINGRPVELIIRDDEQN
VETARKVTRELTESNVEALIGPMTSSVALAMVPIVNQARLVTVSPTVTSTQLSGADDYFLRVLPDTSTYAPKSALFHLKK
SGLRRAAIVYDTGNSSYTTSWLAGFRKSFENSGGSIVVSRSFTSGENIAFSPIVRDLLRHDPDLVVLVCNAVDAALLCQQ
IRKLDRRVTITVSEWGSTERFIELGGSAVEGVYFAQFINHGDVTPRYRNFLASYRTRFGQDPGFPGLAGYDAANVVLDAL
TARKGDASLKDTIVSAGSYQGVQRKIVLDRFGDARSTTFITVVKDGRFVTLE
>Mature_371_residues
PLRIRFLYSLLALGCIALFLSGCERKPIRIGFVGGMSGRVADLGVGGRNGALLAVEERNARGGINGRPVELIIRDDEQNV
ETARKVTRELTESNVEALIGPMTSSVALAMVPIVNQARLVTVSPTVTSTQLSGADDYFLRVLPDTSTYAPKSALFHLKKS
GLRRAAIVYDTGNSSYTTSWLAGFRKSFENSGGSIVVSRSFTSGENIAFSPIVRDLLRHDPDLVVLVCNAVDAALLCQQI
RKLDRRVTITVSEWGSTERFIELGGSAVEGVYFAQFINHGDVTPRYRNFLASYRTRFGQDPGFPGLAGYDAANVVLDALT
ARKGDASLKDTIVSAGSYQGVQRKIVLDRFGDARSTTFITVVKDGRFVTLE

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=352, Percent_Identity=24.7159090909091, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789867, Length=316, Percent_Identity=23.7341772151899, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40397; Mature: 40265

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRIRFLYSLLALGCIALFLSGCERKPIRIGFVGGMSGRVADLGVGGRNGALLAVEERN
CCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEECCC
ARGGINGRPVELIIRDDEQNVETARKVTRELTESNVEALIGPMTSSVALAMVPIVNQARL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHEEECCCCCEE
VTVSPTVTSTQLSGADDYFLRVLPDTSTYAPKSALFHLKKSGLRRAAIVYDTGNSSYTTS
EEECCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
WLAGFRKSFENSGGSIVVSRSFTSGENIAFSPIVRDLLRHDPDLVVLVCNAVDAALLCQQ
HHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
IRKLDRRVTITVSEWGSTERFIELGGSAVEGVYFAQFINHGDVTPRYRNFLASYRTRFGQ
HHHHCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
DPGFPGLAGYDAANVVLDALTARKGDASLKDTIVSAGSYQGVQRKIVLDRFGDARSTTFI
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHEEHHHHCCCCCCEEEE
TVVKDGRFVTLE
EEEECCEEEEEC
>Mature Secondary Structure 
PLRIRFLYSLLALGCIALFLSGCERKPIRIGFVGGMSGRVADLGVGGRNGALLAVEERN
CEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEECCC
ARGGINGRPVELIIRDDEQNVETARKVTRELTESNVEALIGPMTSSVALAMVPIVNQARL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHEEECCCCCEE
VTVSPTVTSTQLSGADDYFLRVLPDTSTYAPKSALFHLKKSGLRRAAIVYDTGNSSYTTS
EEECCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
WLAGFRKSFENSGGSIVVSRSFTSGENIAFSPIVRDLLRHDPDLVVLVCNAVDAALLCQQ
HHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
IRKLDRRVTITVSEWGSTERFIELGGSAVEGVYFAQFINHGDVTPRYRNFLASYRTRFGQ
HHHHCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
DPGFPGLAGYDAANVVLDALTARKGDASLKDTIVSAGSYQGVQRKIVLDRFGDARSTTFI
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHEEHHHHCCCCCCEEEE
TVVKDGRFVTLE
EEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA