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The map label for this gene is pcrA [H]

Identifier: 118580292

GI number: 118580292

Start: 2013688

End: 2015463

Strand: Reverse

Name: pcrA [H]

Synonym: Ppro_1873

Alternate gene names: 118580292

Gene position: 2015463-2013688 (Counterclockwise)

Preceding gene: 118580293

Following gene: 118580291

Centisome position: 50.29

GC content: 36.6

Gene sequence:

>1776_bases
ATGATTCTTGCTAAATTAAACCACGAGCAGAAGTTGGCCGTGAAGCAGGAAGGGCATGTATTATTGACGGCTTGTCCTGG
AAGTGGGAAAACACGAGTATTAATCCATAAACTTGCCTACAACCTCTCAAAGCTTCCTGAAAACAGCAAAAAGCGAATTG
CCGCCGTAACGTTCACAGTTAGGGCTTCGGAAGAAATATTTAGGCGATTAAATGGGATAGGGATTAACAGCAATAATATC
TGGTCTGGCACTCTTCATTCCTTTTGCTTTGAATGGATAATAAAGCCTTATTCATGTTATCTGCCAGAATTGTCAAATGG
GTTTGCAATAGCCGACGAAGCATTTTGTGCGGAATTGGTTAGCAGCCTTAAAGACAAATACAAGCTGAAGCAAATAGATC
CGGTAAATTTCAGAATTAATAGAGATGGAAGTTATGTTGAAAATAAAAGTATTCAACAGAAGCTAATTGCTGAGTATCAC
AGCATCCTTATAAGTAACAAGCTCATAGATTTTGAGCTAATTTTGTATTATTCATACAAATTGCTTGTCACCTACCCGAA
TATCCAAATTACATTATCAAATATATTTGAACTGATATGTGTTGATGAATACCAGGATACTCAGGATTTACTTTATGCAA
TTATCTCGGTAATTATCAAGGCAGGTAACGGAAAGACATCTTTATTTTTTGTCGGGGATACTGACCAAGCTATCTACGCT
TCGCTCGGTGGTGTGGCAAAAAGTTTACAGGAAATTAAAGCCGAAATAGACAATATGCCAATAACTCACTTGACTCTTAC
CGGAAACTATCGGTCAAGCCAAAGAATTATTGATTTTTACTCAAATTTTCAAACTCAAAAGTATCAGATTCATGCTGTCG
GAAAAAACGCCAAGGAAGAAGGGCTTATAACGTTCAATTCAGATATTCATCATTCAAATATTGTTGATGAAATTGCGAAA
TTGATTCAATTAAGTTTAGACACAGGCATTCCAGAAAACGAAATTTGTGTTCTCGTTCCTCAATGGTGGTTAATAACGAT
TGTTACGAAGAAATTGCGTGCATCATTGCCAGGTGTGAGTTTTGACGCATCCGGGTTAGCACCCATGTCAAAAAACAGAG
AAAATATTTGGTATAAACTATCACGTCTTTTGCTTACTGAACCAAATCCGAAAATTTATTCTTCAAGGTACAGATGGGCA
ACTGATTTAATTGATAGTTTCCGTGTTGACACAAATACTGAATTCAATGAACTTTATAAAAATGAACGTGACCTGTTGAG
GCTTATTAATTCCATAAAGTCTTCTGAAATGGAAGGTATTGATTATTTAAACGATTGTTTTGACCAATTTCTGCAAATCA
TAGGCATCGACCAGCTTAAATATCCACAACTTGTAGAACACAGGAACCTGTTCTTCGATAATATTGATAAAAGATTGAAT
GACGATACCTTTAAGGTGCCAAGTGATATTCAATCATTCAAAAGTTTTTACAGGGAAATGACAGGCGTAATAATCAATAC
ATGTGTTGGTGTAAAAGGCGAAGAGTTTGATACGGTCATTTCCTATGGACTGTTAAACGGCTACGTCCCTCATTGGAATG
ATATTTTTTCCGGCAGGGCATCTGATGCAGCAAAAAAATTGTTATATGTTATTTGTTCACGAGCCAAGAAGAATTTACAC
CTGATAAGTGAAATTGGCAGGACAACGAAAAGTGGGTCTGATTTAGGCATTACGCCCGAGCTAAAATGCATCAAATTCGA
GTATGATGTTGTCTAA

Upstream 100 bases:

>100_bases
CCAAAGACTTTGACGTTAAGGATAAATCACATGAAGAGATTGTTTCCGCTTTCATTGCTGATTTCAAAAACGACCAACTT
ACAACCTTTTTAGCATTCCT

Downstream 100 bases:

>100_bases
ACATAAATATTGTTGCACATAAATGATTATTTGGAAGAATTAAATGACAAAAGCTGACATTTTTGAAAAAGTATCCTCGA
ACCTAGGCTACACAAAAAAA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MILAKLNHEQKLAVKQEGHVLLTACPGSGKTRVLIHKLAYNLSKLPENSKKRIAAVTFTVRASEEIFRRLNGIGINSNNI
WSGTLHSFCFEWIIKPYSCYLPELSNGFAIADEAFCAELVSSLKDKYKLKQIDPVNFRINRDGSYVENKSIQQKLIAEYH
SILISNKLIDFELILYYSYKLLVTYPNIQITLSNIFELICVDEYQDTQDLLYAIISVIIKAGNGKTSLFFVGDTDQAIYA
SLGGVAKSLQEIKAEIDNMPITHLTLTGNYRSSQRIIDFYSNFQTQKYQIHAVGKNAKEEGLITFNSDIHHSNIVDEIAK
LIQLSLDTGIPENEICVLVPQWWLITIVTKKLRASLPGVSFDASGLAPMSKNRENIWYKLSRLLLTEPNPKIYSSRYRWA
TDLIDSFRVDTNTEFNELYKNERDLLRLINSIKSSEMEGIDYLNDCFDQFLQIIGIDQLKYPQLVEHRNLFFDNIDKRLN
DDTFKVPSDIQSFKSFYREMTGVIINTCVGVKGEEFDTVISYGLLNGYVPHWNDIFSGRASDAAKKLLYVICSRAKKNLH
LISEIGRTTKSGSDLGITPELKCIKFEYDVV

Sequences:

>Translated_591_residues
MILAKLNHEQKLAVKQEGHVLLTACPGSGKTRVLIHKLAYNLSKLPENSKKRIAAVTFTVRASEEIFRRLNGIGINSNNI
WSGTLHSFCFEWIIKPYSCYLPELSNGFAIADEAFCAELVSSLKDKYKLKQIDPVNFRINRDGSYVENKSIQQKLIAEYH
SILISNKLIDFELILYYSYKLLVTYPNIQITLSNIFELICVDEYQDTQDLLYAIISVIIKAGNGKTSLFFVGDTDQAIYA
SLGGVAKSLQEIKAEIDNMPITHLTLTGNYRSSQRIIDFYSNFQTQKYQIHAVGKNAKEEGLITFNSDIHHSNIVDEIAK
LIQLSLDTGIPENEICVLVPQWWLITIVTKKLRASLPGVSFDASGLAPMSKNRENIWYKLSRLLLTEPNPKIYSSRYRWA
TDLIDSFRVDTNTEFNELYKNERDLLRLINSIKSSEMEGIDYLNDCFDQFLQIIGIDQLKYPQLVEHRNLFFDNIDKRLN
DDTFKVPSDIQSFKSFYREMTGVIINTCVGVKGEEFDTVISYGLLNGYVPHWNDIFSGRASDAAKKLLYVICSRAKKNLH
LISEIGRTTKSGSDLGITPELKCIKFEYDVV
>Mature_591_residues
MILAKLNHEQKLAVKQEGHVLLTACPGSGKTRVLIHKLAYNLSKLPENSKKRIAAVTFTVRASEEIFRRLNGIGINSNNI
WSGTLHSFCFEWIIKPYSCYLPELSNGFAIADEAFCAELVSSLKDKYKLKQIDPVNFRINRDGSYVENKSIQQKLIAEYH
SILISNKLIDFELILYYSYKLLVTYPNIQITLSNIFELICVDEYQDTQDLLYAIISVIIKAGNGKTSLFFVGDTDQAIYA
SLGGVAKSLQEIKAEIDNMPITHLTLTGNYRSSQRIIDFYSNFQTQKYQIHAVGKNAKEEGLITFNSDIHHSNIVDEIAK
LIQLSLDTGIPENEICVLVPQWWLITIVTKKLRASLPGVSFDASGLAPMSKNRENIWYKLSRLLLTEPNPKIYSSRYRWA
TDLIDSFRVDTNTEFNELYKNERDLLRLINSIKSSEMEGIDYLNDCFDQFLQIIGIDQLKYPQLVEHRNLFFDNIDKRLN
DDTFKVPSDIQSFKSFYREMTGVIINTCVGVKGEEFDTVISYGLLNGYVPHWNDIFSGRASDAAKKLLYVICSRAKKNLH
LISEIGRTTKSGSDLGITPELKCIKFEYDVV

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=294, Percent_Identity=26.8707482993197, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 67379; Mature: 67379

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAKLNHEQKLAVKQEGHVLLTACPGSGKTRVLIHKLAYNLSKLPENSKKRIAAVTFTV
CEEEECCCCHHEEEECCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEEEEE
RASEEIFRRLNGIGINSNNIWSGTLHSFCFEWIIKPYSCYLPELSNGFAIADEAFCAELV
ECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCEEECHHHHHHHHH
SSLKDKYKLKQIDPVNFRINRDGSYVENKSIQQKLIAEYHSILISNKLIDFELILYYSYK
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
LLVTYPNIQITLSNIFELICVDEYQDTQDLLYAIISVIIKAGNGKTSLFFVGDTDQAIYA
EEEECCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHH
SLGGVAKSLQEIKAEIDNMPITHLTLTGNYRSSQRIIDFYSNFQTQKYQIHAVGKNAKEE
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCC
GLITFNSDIHHSNIVDEIAKLIQLSLDTGIPENEICVLVPQWWLITIVTKKLRASLPGVS
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCC
FDASGLAPMSKNRENIWYKLSRLLLTEPNPKIYSSRYRWATDLIDSFRVDTNTEFNELYK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHCCCCCCCHHHHHC
NERDLLRLINSIKSSEMEGIDYLNDCFDQFLQIIGIDQLKYPQLVEHRNLFFDNIDKRLN
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
DDTFKVPSDIQSFKSFYREMTGVIINTCVGVKGEEFDTVISYGLLNGYVPHWNDIFSGRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCC
SDAAKKLLYVICSRAKKNLHLISEIGRTTKSGSDLGITPELKCIKFEYDVV
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MILAKLNHEQKLAVKQEGHVLLTACPGSGKTRVLIHKLAYNLSKLPENSKKRIAAVTFTV
CEEEECCCCHHEEEECCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEEEEE
RASEEIFRRLNGIGINSNNIWSGTLHSFCFEWIIKPYSCYLPELSNGFAIADEAFCAELV
ECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCEEECHHHHHHHHH
SSLKDKYKLKQIDPVNFRINRDGSYVENKSIQQKLIAEYHSILISNKLIDFELILYYSYK
HHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
LLVTYPNIQITLSNIFELICVDEYQDTQDLLYAIISVIIKAGNGKTSLFFVGDTDQAIYA
EEEECCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHH
SLGGVAKSLQEIKAEIDNMPITHLTLTGNYRSSQRIIDFYSNFQTQKYQIHAVGKNAKEE
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCC
GLITFNSDIHHSNIVDEIAKLIQLSLDTGIPENEICVLVPQWWLITIVTKKLRASLPGVS
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCC
FDASGLAPMSKNRENIWYKLSRLLLTEPNPKIYSSRYRWATDLIDSFRVDTNTEFNELYK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHCCCCCCCHHHHHC
NERDLLRLINSIKSSEMEGIDYLNDCFDQFLQIIGIDQLKYPQLVEHRNLFFDNIDKRLN
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
DDTFKVPSDIQSFKSFYREMTGVIINTCVGVKGEEFDTVISYGLLNGYVPHWNDIFSGRA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCC
SDAAKKLLYVICSRAKKNLHLISEIGRTTKSGSDLGITPELKCIKFEYDVV
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]