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The map label for this gene is miaB

Identifier: 118580144

GI number: 118580144

Start: 1852075

End: 1853397

Strand: Direct

Name: miaB

Synonym: Ppro_1724

Alternate gene names: 118580144

Gene position: 1852075-1853397 (Clockwise)

Preceding gene: 118580142

Following gene: 118580145

Centisome position: 46.21

GC content: 62.89

Gene sequence:

>1323_bases
ATGACTCAGAACTACGTATACATCGAAACTTTTGGCTGCCAGATGAATGTGAACGATTCCGAGCGAATCCTGACCATGCT
GGCCGATATAGGCTATGTGCCGACCCAGGAGCCGGCCCGTGCCCGCTTGATCCTGCTCAATACCTGCAGCGTGCGTGCAG
GGGCCGAGGAAAAAGTCTACCGTCGCCTGGAAAACCTGGTGGTACTCAAGCGGCACAACAGTCGCCTGATCATCGGCGTC
GGCGGCTGCGTGGCCCAGCAGGAGGGCGAGGCACTCCTGGAGCGCATCCCCAAGCTGGATCTGGTCTTCGGCACCCACAA
CCTGCACCTGCTGAACGACATGGTGCTGGCTGCCGAGCGGGGCGAGCGGAAGAGCGAGACCTCCTTTATCGACAACGACC
AGCGCCTGGACCTCTTTCCCCCCATCAGGGGGACGGCTCGGATCAGCAGCTTCGTGACCGTCATGCAGGGGTGCGAAAAC
TACTGCTCCTACTGTATCGTGCCCTACGTGCGGGGACCGGAGGTCAGCCGCCGCTCCGGGGATATCCTCCGTGAGGTTCG
GCAACTGGCTGATCAGGGGGTACGGGAGGTGGCGCTCCTGGGACAGAATGTCAACTCCTACGGCCTGAAGAGCAGCGCGG
AGCCAAGCTTCGCTGAGCTCATCAGGCTGGTCGCCGCCGTGGACGGCATCAGGCGCATCCGCTTCTTCACCTCCCATCCC
AAGGACATGTCCCCCGAGCTGATCGCCTGTTTCGGTGATCTGCCGGCCCTGTGCAGCCAGCTCCACCTGCCGGCCCAGTC
GGGAAGCGACAACGTGCTTGCACGCATGGGACGGGGCTATACCCGTGAAGAGTACCTGGAGAAGGTCCGGGCACTGCGGG
CGGTCCGTCCCGACATCGTCTTCACCGGCGACATGATCGTCGGTTTTCCGGGCGAAACCGAGGAGGAGTTCCAGGAGACC
CTCTCCCTGATGGAGGAGGTACGGTACATCGACCTGTTTTCCTTCGCCTATTCGCCGCGCCCCGGTACCAGGGCCGCGGA
GTTGGCCGACGACCTGTCCCGCGGGGAAAAGCAGAGTCGCCTGGAGCGGTTGCAGGCGCTCCAGAAGCGCACCACCATGG
AGATCAACGATGTACTGCTCGGTACGCGCCAGACCGTGCTGGTGGAGAGGGAGGGAAAACGCCCCGGCCAGATCTCCGGC
AAGGCCGACAACGGCCGCACCGTCAACTTCAGCGGCGACAGGAGCCTGATCGGTACCTTCGTCGATCTGCGAATCATCCA
GGTGTTCCAGAATTCGCTCCTGGGGGAACTGCTCCCGGGGTGA

Upstream 100 bases:

>100_bases
CGGGATGAAATGGACAGTTTGAAACGGCTTCCGGGATAGGGCTGGGTCAATCCATTGATTGACTTGGAGCGTTTCGTTCG
CGTATATATATTCGTTCGCC

Downstream 100 bases:

>100_bases
CCAACTTGAAATCTTCAGAGGGAAACCATGTCAACAGAAAGCGTAGCTCCATCCAACTTCCTCCGTACGATCATCGATGA
CGACCTGAAAAGCGGCAAGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTQNYVYIETFGCQMNVNDSERILTMLADIGYVPTQEPARARLILLNTCSVRAGAEEKVYRRLENLVVLKRHNSRLIIGV
GGCVAQQEGEALLERIPKLDLVFGTHNLHLLNDMVLAAERGERKSETSFIDNDQRLDLFPPIRGTARISSFVTVMQGCEN
YCSYCIVPYVRGPEVSRRSGDILREVRQLADQGVREVALLGQNVNSYGLKSSAEPSFAELIRLVAAVDGIRRIRFFTSHP
KDMSPELIACFGDLPALCSQLHLPAQSGSDNVLARMGRGYTREEYLEKVRALRAVRPDIVFTGDMIVGFPGETEEEFQET
LSLMEEVRYIDLFSFAYSPRPGTRAAELADDLSRGEKQSRLERLQALQKRTTMEINDVLLGTRQTVLVEREGKRPGQISG
KADNGRTVNFSGDRSLIGTFVDLRIIQVFQNSLLGELLPG

Sequences:

>Translated_440_residues
MTQNYVYIETFGCQMNVNDSERILTMLADIGYVPTQEPARARLILLNTCSVRAGAEEKVYRRLENLVVLKRHNSRLIIGV
GGCVAQQEGEALLERIPKLDLVFGTHNLHLLNDMVLAAERGERKSETSFIDNDQRLDLFPPIRGTARISSFVTVMQGCEN
YCSYCIVPYVRGPEVSRRSGDILREVRQLADQGVREVALLGQNVNSYGLKSSAEPSFAELIRLVAAVDGIRRIRFFTSHP
KDMSPELIACFGDLPALCSQLHLPAQSGSDNVLARMGRGYTREEYLEKVRALRAVRPDIVFTGDMIVGFPGETEEEFQET
LSLMEEVRYIDLFSFAYSPRPGTRAAELADDLSRGEKQSRLERLQALQKRTTMEINDVLLGTRQTVLVEREGKRPGQISG
KADNGRTVNFSGDRSLIGTFVDLRIIQVFQNSLLGELLPG
>Mature_439_residues
TQNYVYIETFGCQMNVNDSERILTMLADIGYVPTQEPARARLILLNTCSVRAGAEEKVYRRLENLVVLKRHNSRLIIGVG
GCVAQQEGEALLERIPKLDLVFGTHNLHLLNDMVLAAERGERKSETSFIDNDQRLDLFPPIRGTARISSFVTVMQGCENY
CSYCIVPYVRGPEVSRRSGDILREVRQLADQGVREVALLGQNVNSYGLKSSAEPSFAELIRLVAAVDGIRRIRFFTSHPK
DMSPELIACFGDLPALCSQLHLPAQSGSDNVLARMGRGYTREEYLEKVRALRAVRPDIVFTGDMIVGFPGETEEEFQETL
SLMEEVRYIDLFSFAYSPRPGTRAAELADDLSRGEKQSRLERLQALQKRTTMEINDVLLGTRQTVLVEREGKRPGQISGK
ADNGRTVNFSGDRSLIGTFVDLRIIQVFQNSLLGELLPG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=37.9454926624738, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=37.9454926624738, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI93277076, Length=401, Percent_Identity=28.6783042394015, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=46.8468468468468, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787057, Length=381, Percent_Identity=29.6587926509186, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=459, Percent_Identity=36.8191721132898, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71996771, Length=362, Percent_Identity=25.9668508287293, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356207, Length=462, Percent_Identity=37.4458874458874, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922432, Length=447, Percent_Identity=28.4116331096197, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PELPD (A1APR6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901394.1
- ProteinModelPortal:   A1APR6
- SMR:   A1APR6
- STRING:   A1APR6
- GeneID:   4572825
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1724
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   A1APR6
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49264; Mature: 49133

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNYVYIETFGCQMNVNDSERILTMLADIGYVPTQEPARARLILLNTCSVRAGAEEKVY
CCCCEEEEEEECEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHH
RRLENLVVLKRHNSRLIIGVGGCVAQQEGEALLERIPKLDLVFGTHNLHLLNDMVLAAER
HHHHHEEEEEECCCEEEEECCCHHCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
GERKSETSFIDNDQRLDLFPPIRGTARISSFVTVMQGCENYCSYCIVPYVRGPEVSRRSG
CCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCH
DILREVRQLADQGVREVALLGQNVNSYGLKSSAEPSFAELIRLVAAVDGIRRIRFFTSHP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
KDMSPELIACFGDLPALCSQLHLPAQSGSDNVLARMGRGYTREEYLEKVRALRAVRPDIV
CCCCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEE
FTGDMIVGFPGETEEEFQETLSLMEEVRYIDLFSFAYSPRPGTRAAELADDLSRGEKQSR
EECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHH
LERLQALQKRTTMEINDVLLGTRQTVLVEREGKRPGQISGKADNGRTVNFSGDRSLIGTF
HHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
VDLRIIQVFQNSLLGELLPG
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQNYVYIETFGCQMNVNDSERILTMLADIGYVPTQEPARARLILLNTCSVRAGAEEKVY
CCCEEEEEEECEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCHHHHH
RRLENLVVLKRHNSRLIIGVGGCVAQQEGEALLERIPKLDLVFGTHNLHLLNDMVLAAER
HHHHHEEEEEECCCEEEEECCCHHCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
GERKSETSFIDNDQRLDLFPPIRGTARISSFVTVMQGCENYCSYCIVPYVRGPEVSRRSG
CCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCH
DILREVRQLADQGVREVALLGQNVNSYGLKSSAEPSFAELIRLVAAVDGIRRIRFFTSHP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
KDMSPELIACFGDLPALCSQLHLPAQSGSDNVLARMGRGYTREEYLEKVRALRAVRPDIV
CCCCCHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCEE
FTGDMIVGFPGETEEEFQETLSLMEEVRYIDLFSFAYSPRPGTRAAELADDLSRGEKQSR
EECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHH
LERLQALQKRTTMEINDVLLGTRQTVLVEREGKRPGQISGKADNGRTVNFSGDRSLIGTF
HHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
VDLRIIQVFQNSLLGELLPG
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA