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The map label for this gene is mscL

Identifier: 118580110

GI number: 118580110

Start: 1815864

End: 1816301

Strand: Direct

Name: mscL

Synonym: Ppro_1689

Alternate gene names: 118580110

Gene position: 1815864-1816301 (Clockwise)

Preceding gene: 118580109

Following gene: 118580111

Centisome position: 45.31

GC content: 55.48

Gene sequence:

>438_bases
ATGCTTAAGGAATTCAAGGAGTTCGCAATCAGGGGGAACGTCATTGACCTGGCAATCGGTATCATCATCGGTGGCGCGTT
CGGCAAGATCGTCGACTCTCTGGTCAAGGACGTGATCATGCCGCCGGTCGGCATGATCATGGGCAAGATGGATTTCGCCT
CGCTGTTCCTCAACCTGTCCGGAACCGAGTACGCGACCCTGGCCGAAGCCAAGAAGGCCGGAGCCGCCACCCTGAATTAT
GGTATATTCATCAGCACCATCGTCGATTTCCTGATTATGGCTTTCGTCGTCTTCCTGATGGTCAAGCAGATCAACCGTAT
GAAGAAGGAGGCTCCCGCTCCTGTTGCTGCATCGGACAGCAAGGACTGCCCCTTCTGTCTTTCACCGATTCCGCTGCAGG
CGACCCGCTGCCCGCACTGCACGTCAAACCTGAAGTAA

Upstream 100 bases:

>100_bases
GAATCGGGACCAGGGCAGTATCTGGTTCCTTCCCGCCGTGGTTGAAGTAGCCGACGACGAGAGCGTCGCGGACATATCTG
CTATCTAGAGGAGGCGTACC

Downstream 100 bases:

>100_bases
GCAAGAGGGAAACAGCCTCTTTCATGTCTAAAAACGGACGCTTTTGATGAATCTGTCGGCTGTTGTTGAAAGTGTCATCT
TCAGCGCGGACACGCCCCTG

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MLKEFKEFAIRGNVIDLAIGIIIGGAFGKIVDSLVKDVIMPPVGMIMGKMDFASLFLNLSGTEYATLAEAKKAGAATLNY
GIFISTIVDFLIMAFVVFLMVKQINRMKKEAPAPVAASDSKDCPFCLSPIPLQATRCPHCTSNLK

Sequences:

>Translated_145_residues
MLKEFKEFAIRGNVIDLAIGIIIGGAFGKIVDSLVKDVIMPPVGMIMGKMDFASLFLNLSGTEYATLAEAKKAGAATLNY
GIFISTIVDFLIMAFVVFLMVKQINRMKKEAPAPVAASDSKDCPFCLSPIPLQATRCPHCTSNLK
>Mature_145_residues
MLKEFKEFAIRGNVIDLAIGIIIGGAFGKIVDSLVKDVIMPPVGMIMGKMDFASLFLNLSGTEYATLAEAKKAGAATLNY
GIFISTIVDFLIMAFVVFLMVKQINRMKKEAPAPVAASDSKDCPFCLSPIPLQATRCPHCTSNLK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=117, Percent_Identity=46.1538461538462, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PELPD (A1APN2)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_901360.1
- ProteinModelPortal:   A1APN2
- SMR:   A1APN2
- STRING:   A1APN2
- GeneID:   4572424
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1689
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   A1APN2
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15648; Mature: 15648

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x248154c0)-; HASH(0x22a1941c)-;

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKEFAIRGNVIDLAIGIIIGGAFGKIVDSLVKDVIMPPVGMIMGKMDFASLFLNLS
CCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GTEYATLAEAKKAGAATLNYGIFISTIVDFLIMAFVVFLMVKQINRMKKEAPAPVAASDS
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KDCPFCLSPIPLQATRCPHCTSNLK
CCCCEECCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MLKEFKEFAIRGNVIDLAIGIIIGGAFGKIVDSLVKDVIMPPVGMIMGKMDFASLFLNLS
CCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GTEYATLAEAKKAGAATLNYGIFISTIVDFLIMAFVVFLMVKQINRMKKEAPAPVAASDS
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KDCPFCLSPIPLQATRCPHCTSNLK
CCCCEECCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA