Click here to switch to the map view.

The map label for this gene is tig

Identifier: 118579614

GI number: 118579614

Start: 1235881

End: 1237194

Strand: Reverse

Name: tig

Synonym: Ppro_1184

Alternate gene names: 118579614

Gene position: 1237194-1235881 (Counterclockwise)

Preceding gene: 118579615

Following gene: 118579613

Centisome position: 30.87

GC content: 59.44

Gene sequence:

>1314_bases
ATGCAGGTTAACGTCGAAACCATCAGCCCCGTTAGCAAGAAAGTCAGCATCGAGATCCCTGCCGACCAGGTTAACAGCGA
AATTGAGAAGACCTACGCCACCATCCAGAAGCGCGCCAAGATCCAGGGATTCCGCCCCGGCAAAGCGCCCCTGCAGCTGA
TAAAACGCACCTACGCCGACTCCATGCGCGACGAAGTCATGCGGCGTTTCTACGAGACGACCCTCTTCAAGACCCTGGCG
GAACACAAAATCGAGCCGATCGACGCCCCGACCGTTGAATGTGACATCCTGGAAGAAGGAACCCCCTTCAAGTACAGCGC
CCTGGTGGAAGTGATGCCGGAGGTCCTGCTCACCCAGTACACCGGCCTTGAGATCACCAAGGAACGCTACGTGTTCGATC
CCCAGAAGATCGAGGACGAAATCGCCCGCATGCGCGACGGCATGGCCCAACTGGTGCCGGTGGAAGGCGATGTGGCCGTA
GAAATGGGGCACATGGTCACCATCGACTACGCCTTTTCCGTGGACGGCTTCCCCGAAGAGAGCACCTCCGCCGAGAACGC
CGTCGTGGAAGTCGGCGCCCATCGCCTGCTGCCGGAATTCGAAGACCAGCTGGTGGGCATGAAGTGTGGTGAATCGAAAC
ATGTCACCGTGACCCTACCCGAGGCCTACCGCAATCCCGAGGTTGCCGGCAAGGAAGGCGCCTTCATGGTCACGCTCAAC
GAGATCAAGCGCAAGGAGCTTCCCGAGCTCAACGACGAGTTCGCTCAGCAGTTCGGTGAGTTCGAGACCGTCGAGCAACT
GCGCGAAAAGATGACCGAATACCACCAGCAGCACGAACAGGACCGCATCGAGCAGGAGCAGCGCGAGACCGTCATCCAGG
CGCTGATCGAGAAGAACCCGCTGGATGTCCCCCAATCCATGGTCAAGCGCCAGGTGGAGCAGATGCTGCAGAACCTGAAG
AACCGCCTCAAGAGCCGCAACATGTCCATGGAGATGATGGGGATGGACGACGACAGCTTCCGCGAAAGAGTTCGCGACTC
CGCGACCGACAAGGTCCGCGGTGGCCTGCTGCTCATGGCCCTGATCGAGAAAGAGGATTTCAGCGTCAGCGACGAGGAGA
TCGAGAAACGCTATGAGAAGCTGGCAGGCGGCAACGAAGAGATGTTGGAGCGGATCAAGGAGCACTACACCAGCAGCCCT
TCGGTACGCCACTCACTGATCGCCGAAATCAAGGAAGACAAGGCGGTCCGTTTTCTGCTGGACAACGCCGTGATCACCGA
GACAGACCCCGCCGAAACCGCGGCGGAGGCCTAG

Upstream 100 bases:

>100_bases
GGTATAGGAGTTCAAGTCTCCTCTCTCGCACCATGCGCCTCCCCTTTACCTTTACAGTTTATTTTCAGTAACCAATTCCA
TCCATGTGAGGTTATCAGCA

Downstream 100 bases:

>100_bases
TCCGCCAGCGGGCGAAACTCACGACAGCAGATAGATCGAGGCCAAGGGGATCTCCGATCCGGCCGTGGAACCGGCCAAGC
ACGTCACACGGCATGCGTCA

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MQVNVETISPVSKKVSIEIPADQVNSEIEKTYATIQKRAKIQGFRPGKAPLQLIKRTYADSMRDEVMRRFYETTLFKTLA
EHKIEPIDAPTVECDILEEGTPFKYSALVEVMPEVLLTQYTGLEITKERYVFDPQKIEDEIARMRDGMAQLVPVEGDVAV
EMGHMVTIDYAFSVDGFPEESTSAENAVVEVGAHRLLPEFEDQLVGMKCGESKHVTVTLPEAYRNPEVAGKEGAFMVTLN
EIKRKELPELNDEFAQQFGEFETVEQLREKMTEYHQQHEQDRIEQEQRETVIQALIEKNPLDVPQSMVKRQVEQMLQNLK
NRLKSRNMSMEMMGMDDDSFRERVRDSATDKVRGGLLLMALIEKEDFSVSDEEIEKRYEKLAGGNEEMLERIKEHYTSSP
SVRHSLIAEIKEDKAVRFLLDNAVITETDPAETAAEA

Sequences:

>Translated_437_residues
MQVNVETISPVSKKVSIEIPADQVNSEIEKTYATIQKRAKIQGFRPGKAPLQLIKRTYADSMRDEVMRRFYETTLFKTLA
EHKIEPIDAPTVECDILEEGTPFKYSALVEVMPEVLLTQYTGLEITKERYVFDPQKIEDEIARMRDGMAQLVPVEGDVAV
EMGHMVTIDYAFSVDGFPEESTSAENAVVEVGAHRLLPEFEDQLVGMKCGESKHVTVTLPEAYRNPEVAGKEGAFMVTLN
EIKRKELPELNDEFAQQFGEFETVEQLREKMTEYHQQHEQDRIEQEQRETVIQALIEKNPLDVPQSMVKRQVEQMLQNLK
NRLKSRNMSMEMMGMDDDSFRERVRDSATDKVRGGLLLMALIEKEDFSVSDEEIEKRYEKLAGGNEEMLERIKEHYTSSP
SVRHSLIAEIKEDKAVRFLLDNAVITETDPAETAAEA
>Mature_437_residues
MQVNVETISPVSKKVSIEIPADQVNSEIEKTYATIQKRAKIQGFRPGKAPLQLIKRTYADSMRDEVMRRFYETTLFKTLA
EHKIEPIDAPTVECDILEEGTPFKYSALVEVMPEVLLTQYTGLEITKERYVFDPQKIEDEIARMRDGMAQLVPVEGDVAV
EMGHMVTIDYAFSVDGFPEESTSAENAVVEVGAHRLLPEFEDQLVGMKCGESKHVTVTLPEAYRNPEVAGKEGAFMVTLN
EIKRKELPELNDEFAQQFGEFETVEQLREKMTEYHQQHEQDRIEQEQRETVIQALIEKNPLDVPQSMVKRQVEQMLQNLK
NRLKSRNMSMEMMGMDDDSFRERVRDSATDKVRGGLLLMALIEKEDFSVSDEEIEKRYEKLAGGNEEMLERIKEHYTSSP
SVRHSLIAEIKEDKAVRFLLDNAVITETDPAETAAEA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=429, Percent_Identity=29.1375291375291, Blast_Score=178, Evalue=8e-46,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PELPD (A1AN86)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900864.1
- ProteinModelPortal:   A1AN86
- STRING:   A1AN86
- GeneID:   4574654
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1184
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   A1AN86
- ProtClustDB:   CLSK2319998
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 49991; Mature: 49991

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVNVETISPVSKKVSIEIPADQVNSEIEKTYATIQKRAKIQGFRPGKAPLQLIKRTYAD
CEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SMRDEVMRRFYETTLFKTLAEHKIEPIDAPTVECDILEEGTPFKYSALVEVMPEVLLTQY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
TGLEITKERYVFDPQKIEDEIARMRDGMAQLVPVEGDVAVEMGHMVTIDYAFSVDGFPEE
CCCEEEHHHHCCCHHHHHHHHHHHHCCHHHEECCCCCEEEEECCEEEEEEEEECCCCCCC
STSAENAVVEVGAHRLLPEFEDQLVGMKCGESKHVTVTLPEAYRNPEVAGKEGAFMVTLN
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCEEEEHH
EIKRKELPELNDEFAQQFGEFETVEQLREKMTEYHQQHEQDRIEQEQRETVIQALIEKNP
HHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDVPQSMVKRQVEQMLQNLKNRLKSRNMSMEMMGMDDDSFRERVRDSATDKVRGGLLLMA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHH
LIEKEDFSVSDEEIEKRYEKLAGGNEEMLERIKEHYTSSPSVRHSLIAEIKEDKAVRFLL
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHH
DNAVITETDPAETAAEA
CCCEEECCCCCHHCCCC
>Mature Secondary Structure
MQVNVETISPVSKKVSIEIPADQVNSEIEKTYATIQKRAKIQGFRPGKAPLQLIKRTYAD
CEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SMRDEVMRRFYETTLFKTLAEHKIEPIDAPTVECDILEEGTPFKYSALVEVMPEVLLTQY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
TGLEITKERYVFDPQKIEDEIARMRDGMAQLVPVEGDVAVEMGHMVTIDYAFSVDGFPEE
CCCEEEHHHHCCCHHHHHHHHHHHHCCHHHEECCCCCEEEEECCEEEEEEEEECCCCCCC
STSAENAVVEVGAHRLLPEFEDQLVGMKCGESKHVTVTLPEAYRNPEVAGKEGAFMVTLN
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCEEEEHH
EIKRKELPELNDEFAQQFGEFETVEQLREKMTEYHQQHEQDRIEQEQRETVIQALIEKNP
HHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDVPQSMVKRQVEQMLQNLKNRLKSRNMSMEMMGMDDDSFRERVRDSATDKVRGGLLLMA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHH
LIEKEDFSVSDEEIEKRYEKLAGGNEEMLERIKEHYTSSPSVRHSLIAEIKEDKAVRFLL
HHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHH
DNAVITETDPAETAAEA
CCCEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA