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The map label for this gene is bioB

Identifier: 118579605

GI number: 118579605

Start: 1227901

End: 1228887

Strand: Direct

Name: bioB

Synonym: Ppro_1175

Alternate gene names: 118579605

Gene position: 1227901-1228887 (Clockwise)

Preceding gene: 118579604

Following gene: 118579606

Centisome position: 30.64

GC content: 63.63

Gene sequence:

>987_bases
ATGAGATCATTCATACTTGCCTGCGCCGACCGGGTAGTTGAAGGCGCCACACTCGGGGCTGAGGAGGCACTCAGGCTGGC
CGAAGCCCGTGGAGCCGACCTGCACCTGCTGTTCGCCGAGGCGTCGCGCATCCGCCAGCACTTCACCGGAAACTCGGCCT
CGCTCTGCTCGATCATCAACGCCAAATCGGGCCGCTGCGCGGAAAATTGCGCCTTCTGCGCCCAGTCCGCAGCCCACAAG
ACCGATGCACAGGTCTACCCCCTTGTGGACGAGGAGGAGATCGTCCGCTGTGCCAGGGATGCCGAACGCAACGGCGCCCG
CTGCTACGGCATCGTCACCAGCGGAACCGGCATCAGGCCGGGAGCGGAGCTGGAAACCATCTGCAACTCCCTGCGACGCA
TCAGGTCGGAGACAGCCATAGCCCCCTCCTGCTCCCTTGGAATCCTGGACGAGGAGACGGCCCGCCTGCTCAAGGATGCG
GGCATGGTCACCTATCACCACAACCTGGAGACCTCGCGCAGTTTCTTCCCCTCCATCTGCAGCAGCCACGACTATGAGCA
GGATGTGGAAACGATTCGGGCGGTGAAGCGGGCCGGGGTGAGGGTCTGCTGCGGCGGAATCTTCGGCCTTGGCGAAAGCT
TCGGCCAGCGCATCGAGATGACCGAAACCCTACGGGAACTGGACGTGGACTCGGTGCCGCTCAACTTCCTCAATCCGGTG
GAGGGGACTCGGCTGGAGAAGGCCGACTTCCTGACCCCCCTGGAGTGCCTGAAGACCATCGCCATCTACCGCTTCATGCT
GCCGGGAAAGAGCCTGTCGGTCTGCGGCGGTCGCGAAACGAACCTGCGCGAGTTGCAGTCCTGGATCTTTCTGGCCGGCG
CCAGCGGCATGATGACCGGTAATTACCTGACCACCCTGGGGCGGAAGCCGGAACAGGACCACCAGATGCTGGCTGATCTG
GGCATGGGCGTGGGAGGGTGTTCATGA

Upstream 100 bases:

>100_bases
AACTCAGGATGGACGGATATGATCGTTAACGACATGTCGGTCGCGGGTTGACAATGGGGAGGCGCTGTGCTATCGCTGGT
CGCGGAGGGTAACCATCATT

Downstream 100 bases:

>100_bases
GCAGGGGGATCTTCATAACCGGGACCGATACCGGTGTGGGCAAGACCATCGTGGCAGCCACCCTGGCCCGGCTGTTGCGC
ATGAGGGGGGTCGATGTGGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRSFILACADRVVEGATLGAEEALRLAEARGADLHLLFAEASRIRQHFTGNSASLCSIINAKSGRCAENCAFCAQSAAHK
TDAQVYPLVDEEEIVRCARDAERNGARCYGIVTSGTGIRPGAELETICNSLRRIRSETAIAPSCSLGILDEETARLLKDA
GMVTYHHNLETSRSFFPSICSSHDYEQDVETIRAVKRAGVRVCCGGIFGLGESFGQRIEMTETLRELDVDSVPLNFLNPV
EGTRLEKADFLTPLECLKTIAIYRFMLPGKSLSVCGGRETNLRELQSWIFLAGASGMMTGNYLTTLGRKPEQDHQMLADL
GMGVGGCS

Sequences:

>Translated_328_residues
MRSFILACADRVVEGATLGAEEALRLAEARGADLHLLFAEASRIRQHFTGNSASLCSIINAKSGRCAENCAFCAQSAAHK
TDAQVYPLVDEEEIVRCARDAERNGARCYGIVTSGTGIRPGAELETICNSLRRIRSETAIAPSCSLGILDEETARLLKDA
GMVTYHHNLETSRSFFPSICSSHDYEQDVETIRAVKRAGVRVCCGGIFGLGESFGQRIEMTETLRELDVDSVPLNFLNPV
EGTRLEKADFLTPLECLKTIAIYRFMLPGKSLSVCGGRETNLRELQSWIFLAGASGMMTGNYLTTLGRKPEQDHQMLADL
GMGVGGCS
>Mature_328_residues
MRSFILACADRVVEGATLGAEEALRLAEARGADLHLLFAEASRIRQHFTGNSASLCSIINAKSGRCAENCAFCAQSAAHK
TDAQVYPLVDEEEIVRCARDAERNGARCYGIVTSGTGIRPGAELETICNSLRRIRSETAIAPSCSLGILDEETARLLKDA
GMVTYHHNLETSRSFFPSICSSHDYEQDVETIRAVKRAGVRVCCGGIFGLGESFGQRIEMTETLRELDVDSVPLNFLNPV
EGTRLEKADFLTPLECLKTIAIYRFMLPGKSLSVCGGRETNLRELQSWIFLAGASGMMTGNYLTTLGRKPEQDHQMLADL
GMGVGGCS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=295, Percent_Identity=32.2033898305085, Blast_Score=162, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321725, Length=301, Percent_Identity=31.2292358803987, Blast_Score=155, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PELPD (A1AN77)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900855.1
- ProteinModelPortal:   A1AN77
- SMR:   A1AN77
- STRING:   A1AN77
- GeneID:   4571524
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1175
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   TPLMELV
- PhylomeDB:   A1AN77
- ProtClustDB:   CLSK828421
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35655; Mature: 35655

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFILACADRVVEGATLGAEEALRLAEARGADLHLLFAEASRIRQHFTGNSASLCSIIN
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHC
AKSGRCAENCAFCAQSAAHKTDAQVYPLVDEEEIVRCARDAERNGARCYGIVTSGTGIRP
CCCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GAELETICNSLRRIRSETAIAPSCSLGILDEETARLLKDAGMVTYHHNLETSRSFFPSIC
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHH
SSHDYEQDVETIRAVKRAGVRVCCGGIFGLGESFGQRIEMTETLRELDVDSVPLNFLNPV
CCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTRLEKADFLTPLECLKTIAIYRFMLPGKSLSVCGGRETNLRELQSWIFLAGASGMMTG
CCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCC
NYLTTLGRKPEQDHQMLADLGMGVGGCS
HHHHHCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSFILACADRVVEGATLGAEEALRLAEARGADLHLLFAEASRIRQHFTGNSASLCSIIN
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHC
AKSGRCAENCAFCAQSAAHKTDAQVYPLVDEEEIVRCARDAERNGARCYGIVTSGTGIRP
CCCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GAELETICNSLRRIRSETAIAPSCSLGILDEETARLLKDAGMVTYHHNLETSRSFFPSIC
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHH
SSHDYEQDVETIRAVKRAGVRVCCGGIFGLGESFGQRIEMTETLRELDVDSVPLNFLNPV
CCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
EGTRLEKADFLTPLECLKTIAIYRFMLPGKSLSVCGGRETNLRELQSWIFLAGASGMMTG
CCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCC
NYLTTLGRKPEQDHQMLADLGMGVGGCS
HHHHHCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA