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The map label for this gene is kefA [C]

Identifier: 118579578

GI number: 118579578

Start: 1193130

End: 1194230

Strand: Direct

Name: kefA [C]

Synonym: Ppro_1146

Alternate gene names: 118579578

Gene position: 1193130-1194230 (Clockwise)

Preceding gene: 118579577

Following gene: 118579579

Centisome position: 29.77

GC content: 59.58

Gene sequence:

>1101_bases
GTGAATAGCGGACTGCTCACATTGCTCCAGAGCTTCCTCCGGCCCCAGGAAACCGACGGAATCCTGAGGGTATGGGCCAA
AGAGCTGCTGGTGGCGCTTTTGATCCTGACCTGCTTCTGGCTGCTGGCTCAAGCAGCCTGCTGGATCCTGAACAGGTGGG
GGCGCAGGCTTACCACCTTTACCCGCTCCGATCTTGACGATCGCATTCTGCAGCGGCTTATTCCCCATGTGTCGCGTCTC
TTGACCATGCTGGGGCTCTACCTGGCCATGCACTCCCTGCCGCTGAACGAACGGCTGGTGGTGATCTTCTCCGGCCTGCT
TTACGTCGTGCTGGTGGCGGTCTTCTTCAACCTGGTGTACCATGCCCTGGACGAACTGATACAGTGGTACATCGCCGGTC
AGCGTGAGCGGAGCGATGCCCTGATCTCGCGCCAGATGCTGCCGGTTGCCGAAAAGCTGGCCCTGCTGCTCCTCATGGGT
ACGGCCCTAATCATCATCCTCAAACATTTTAACTATGATATCTTCTCGGTGGTCACCGCCCTGGGGATCGGCTCCCTGGC
CATCGGTTTGGCGGCCAAGGACACCCTGGCCCACATGATCTCCGGGTTCACCCTGATGCTTGACCGCCCCTTCAGGATCG
GCGACCGTATCCAGCTGGCAGGGGGGCAGACCGGCGATGTGCTGGACATCGGCCTGCGCAGCACCAAGATCATGACCCTC
TCCAACCAGCAGCTGGTGATCCCCAACTCGGAACTGTGCAACAGCATGCTGACCAACCTGGCTGCTCCCGATGTGCGGGC
CAAGGGGCGCATCAACATCGGCGTGGCCTATGGCAGCGATGTGGAACGGGTCAAGCGGGTGCTGGTGGATACGGCCCTGG
CGGTGGATGATGTGCTGGCTGACCCGCCGCCGGAGGCGTTTTTCGTTGCCTTCGGAGACTCATCTCTCAGCATGGCCCTG
TTCTTCTGGGTCGAGAGTTACGAAATCCTGTTTACGACCACCGACAGGATCAATTCGCTGATCCTGCGTCGATTTGCCGA
CAACGCTATTGAGATACCGTTCCCCACCCGGACGGTGATTATGGAAAAAGGACTGGACTGA

Upstream 100 bases:

>100_bases
CAGGAGGTGGTGGGCGCCCTGGGCGAGGCCAAAATCCGCGAGTCCTTCGACCTGAACTACCACCTGAAGCACGTGAACAC
CATCTTCAAACGGGTATTCG

Downstream 100 bases:

>100_bases
TATGGCCAACAGAATAGAAATCGCCCTGAAGGAGGGAGTTCGCGACGCCCGCGGTGAGCGGATCAAGCGGGAGATCGAAC
ACTTTTTGCACCTGGCTGTG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNSGLLTLLQSFLRPQETDGILRVWAKELLVALLILTCFWLLAQAACWILNRWGRRLTTFTRSDLDDRILQRLIPHVSRL
LTMLGLYLAMHSLPLNERLVVIFSGLLYVVLVAVFFNLVYHALDELIQWYIAGQRERSDALISRQMLPVAEKLALLLLMG
TALIIILKHFNYDIFSVVTALGIGSLAIGLAAKDTLAHMISGFTLMLDRPFRIGDRIQLAGGQTGDVLDIGLRSTKIMTL
SNQQLVIPNSELCNSMLTNLAAPDVRAKGRINIGVAYGSDVERVKRVLVDTALAVDDVLADPPPEAFFVAFGDSSLSMAL
FFWVESYEILFTTTDRINSLILRRFADNAIEIPFPTRTVIMEKGLD

Sequences:

>Translated_366_residues
MNSGLLTLLQSFLRPQETDGILRVWAKELLVALLILTCFWLLAQAACWILNRWGRRLTTFTRSDLDDRILQRLIPHVSRL
LTMLGLYLAMHSLPLNERLVVIFSGLLYVVLVAVFFNLVYHALDELIQWYIAGQRERSDALISRQMLPVAEKLALLLLMG
TALIIILKHFNYDIFSVVTALGIGSLAIGLAAKDTLAHMISGFTLMLDRPFRIGDRIQLAGGQTGDVLDIGLRSTKIMTL
SNQQLVIPNSELCNSMLTNLAAPDVRAKGRINIGVAYGSDVERVKRVLVDTALAVDDVLADPPPEAFFVAFGDSSLSMAL
FFWVESYEILFTTTDRINSLILRRFADNAIEIPFPTRTVIMEKGLD
>Mature_366_residues
MNSGLLTLLQSFLRPQETDGILRVWAKELLVALLILTCFWLLAQAACWILNRWGRRLTTFTRSDLDDRILQRLIPHVSRL
LTMLGLYLAMHSLPLNERLVVIFSGLLYVVLVAVFFNLVYHALDELIQWYIAGQRERSDALISRQMLPVAEKLALLLLMG
TALIIILKHFNYDIFSVVTALGIGSLAIGLAAKDTLAHMISGFTLMLDRPFRIGDRIQLAGGQTGDVLDIGLRSTKIMTL
SNQQLVIPNSELCNSMLTNLAAPDVRAKGRINIGVAYGSDVERVKRVLVDTALAVDDVLADPPPEAFFVAFGDSSLSMAL
FFWVESYEILFTTTDRINSLILRRFADNAIEIPFPTRTVIMEKGLD

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=182, Percent_Identity=33.5164835164835, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789291, Length=227, Percent_Identity=30.8370044052863, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787591, Length=278, Percent_Identity=30.2158273381295, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367355, Length=179, Percent_Identity=26.2569832402235, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 40905; Mature: 40905

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSGLLTLLQSFLRPQETDGILRVWAKELLVALLILTCFWLLAQAACWILNRWGRRLTTF
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRSDLDDRILQRLIPHVSRLLTMLGLYLAMHSLPLNERLVVIFSGLLYVVLVAVFFNLVY
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HALDELIQWYIAGQRERSDALISRQMLPVAEKLALLLLMGTALIIILKHFNYDIFSVVTA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGIGSLAIGLAAKDTLAHMISGFTLMLDRPFRIGDRIQLAGGQTGDVLDIGLRSTKIMTL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCEEEECCCCEEEEEE
SNQQLVIPNSELCNSMLTNLAAPDVRAKGRINIGVAYGSDVERVKRVLVDTALAVDDVLA
CCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHC
DPPPEAFFVAFGDSSLSMALFFWVESYEILFTTTDRINSLILRRFADNAIEIPFPTRTVI
CCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCEECCCCCHHHH
MEKGLD
HCCCCC
>Mature Secondary Structure
MNSGLLTLLQSFLRPQETDGILRVWAKELLVALLILTCFWLLAQAACWILNRWGRRLTTF
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRSDLDDRILQRLIPHVSRLLTMLGLYLAMHSLPLNERLVVIFSGLLYVVLVAVFFNLVY
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HALDELIQWYIAGQRERSDALISRQMLPVAEKLALLLLMGTALIIILKHFNYDIFSVVTA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGIGSLAIGLAAKDTLAHMISGFTLMLDRPFRIGDRIQLAGGQTGDVLDIGLRSTKIMTL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCEEEECCCCEEEEEE
SNQQLVIPNSELCNSMLTNLAAPDVRAKGRINIGVAYGSDVERVKRVLVDTALAVDDVLA
CCCEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHC
DPPPEAFFVAFGDSSLSMALFFWVESYEILFTTTDRINSLILRRFADNAIEIPFPTRTVI
CCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCEECCCCCHHHH
MEKGLD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]