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The map label for this gene is purB [H]

Identifier: 118579577

GI number: 118579577

Start: 1191842

End: 1193137

Strand: Direct

Name: purB [H]

Synonym: Ppro_1145

Alternate gene names: 118579577

Gene position: 1191842-1193137 (Clockwise)

Preceding gene: 118579576

Following gene: 118579578

Centisome position: 29.74

GC content: 63.12

Gene sequence:

>1296_bases
ATGATCGAGCGCTATTCCCGCCCCGAGATGGCCCACATCTGGACCGCCCAAAACCGTTTCAAGATCTGGCTGGAGATAGA
GACCCTGGCCTGCGAGGCCCAGGCGGAACTGGGGGTCATCCCCAAGGACGTGGTGCCGGTGATCCGTGAGAAGGGCAATT
TCGACATCGCCCGCATCGACGCCATCGAGGCCGAAGTCAAGCACGACGTGATCGCCTTTCTGACCTCGGTGTCCGAATAT
GTGGGGCCCGAGGCGCGTTTCATTCACCAGGGGATGACCTCTTCAGATGTCCTCGATACCTGCCTGTCGGTGCAGTTGAC
CCAGGCGGCCGACCAGCTGCTGGTGGACCTGGACATGGTGCTGGCTTCCATCAGGAAGCGCGCCCTGGAACACCGCGACA
CGGTCTGCATCGGCCGCTCCCACGGCATTCACGCCGAGCCGGTCACCTTCGGCCTCAAGCTGGCCACCTGGTACGCCGAG
ATGCAGAGGAACCGTGACCGCCTGGCCGCCAGCCGCGAGACCATCGCCACCGGCGCCATCTCCGGCGCGGTGGGCACCTT
TGCCAACATCGACCCCTTTGTTGAGGAGTACGTCTGCCAGAGGATGGGGCTCAAGGCCGAGCCGGTCTCCACCCAGGTCA
TCCCCCGCGACCGCCATGCAGAATACTTTTGCACCCTGGCCCTGATCGCCTCCTCCATGGAGCGCATCGCCGTTGAAATC
CGCCACCTGCAGCGCACCGAGGTGCTGGAGGCGGAGGAGTTCTTCAGCAAGGGGCAGAAGGGGTCGTCGGCCATGCCCCA
CAAGCGCAACCCGGTGCTCTCCGAGAACCTCACCGGTCAGGCGCGCTACATCCGCTCCCTCTGCATTCCGGCCCTGGAAA
ACGTGGCTCTCTGGCACGAGCGGGACATCTCCCACTCCTCGGTGGAGCGTTACATCGGCCCGGACGCCACCGTGGCCCTG
GACTTCTCCCTGCGCCGTCTGAACGGCCTGATCGAGAACCTGGTGGTCTATCCCGAAAACATGCTCAGGAACCTGAACCA
GATGAAGGGGCTGGTCTTCTCCCAGAAGATTCTCCTGGACCTGACCCAGGCCGGCGTCTCCCGGGAGGATTCCTACCGTC
TGGTGCAGCGCAACGCCATGAAGGTCTGGGAGCAGGGCAAGGACTTCTTGACCGAGCTGTTGAACGACCAGGAGGTGGTG
GGCGCCCTGGGCGAGGCCAAAATCCGCGAGTCCTTCGACCTGAACTACCACCTGAAGCACGTGAACACCATCTTCAAACG
GGTATTCGGTGAATAG

Upstream 100 bases:

>100_bases
GCGTGAACCTGCCTCCCCGGCTCTTCGTCTCAAAATAACGGGCAACCACAAGGGATGCCCCTGCATTGACCAAAACCAAT
CAGAGGAGAACATACAGACA

Downstream 100 bases:

>100_bases
CGGACTGCTCACATTGCTCCAGAGCTTCCTCCGGCCCCAGGAAACCGACGGAATCCTGAGGGTATGGGCCAAAGAGCTGC
TGGTGGCGCTTTTGATCCTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYSRPEMAHIWTAQNRFKIWLEIETLACEAQAELGVIPKDVVPVIREKGNFDIARIDAIEAEVKHDVIAFLTSVSEY
VGPEARFIHQGMTSSDVLDTCLSVQLTQAADQLLVDLDMVLASIRKRALEHRDTVCIGRSHGIHAEPVTFGLKLATWYAE
MQRNRDRLAASRETIATGAISGAVGTFANIDPFVEEYVCQRMGLKAEPVSTQVIPRDRHAEYFCTLALIASSMERIAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGQARYIRSLCIPALENVALWHERDISHSSVERYIGPDATVAL
DFSLRRLNGLIENLVVYPENMLRNLNQMKGLVFSQKILLDLTQAGVSREDSYRLVQRNAMKVWEQGKDFLTELLNDQEVV
GALGEAKIRESFDLNYHLKHVNTIFKRVFGE

Sequences:

>Translated_431_residues
MIERYSRPEMAHIWTAQNRFKIWLEIETLACEAQAELGVIPKDVVPVIREKGNFDIARIDAIEAEVKHDVIAFLTSVSEY
VGPEARFIHQGMTSSDVLDTCLSVQLTQAADQLLVDLDMVLASIRKRALEHRDTVCIGRSHGIHAEPVTFGLKLATWYAE
MQRNRDRLAASRETIATGAISGAVGTFANIDPFVEEYVCQRMGLKAEPVSTQVIPRDRHAEYFCTLALIASSMERIAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGQARYIRSLCIPALENVALWHERDISHSSVERYIGPDATVAL
DFSLRRLNGLIENLVVYPENMLRNLNQMKGLVFSQKILLDLTQAGVSREDSYRLVQRNAMKVWEQGKDFLTELLNDQEVV
GALGEAKIRESFDLNYHLKHVNTIFKRVFGE
>Mature_431_residues
MIERYSRPEMAHIWTAQNRFKIWLEIETLACEAQAELGVIPKDVVPVIREKGNFDIARIDAIEAEVKHDVIAFLTSVSEY
VGPEARFIHQGMTSSDVLDTCLSVQLTQAADQLLVDLDMVLASIRKRALEHRDTVCIGRSHGIHAEPVTFGLKLATWYAE
MQRNRDRLAASRETIATGAISGAVGTFANIDPFVEEYVCQRMGLKAEPVSTQVIPRDRHAEYFCTLALIASSMERIAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGQARYIRSLCIPALENVALWHERDISHSSVERYIGPDATVAL
DFSLRRLNGLIENLVVYPENMLRNLNQMKGLVFSQKILLDLTQAGVSREDSYRLVQRNAMKVWEQGKDFLTELLNDQEVV
GALGEAKIRESFDLNYHLKHVNTIFKRVFGE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=28.5714285714286, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1787376, Length=317, Percent_Identity=28.391167192429, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=26.0759493670886, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=333, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=27.9487179487179, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24647570, Length=403, Percent_Identity=27.0471464019851, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48763; Mature: 48763

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPEMAHIWTAQNRFKIWLEIETLACEAQAELGVIPKDVVPVIREKGNFDIARID
CCCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEHH
AIEAEVKHDVIAFLTSVSEYVGPEARFIHQGMTSSDVLDTCLSVQLTQAADQLLVDLDMV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASIRKRALEHRDTVCIGRSHGIHAEPVTFGLKLATWYAEMQRNRDRLAASRETIATGAI
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAVGTFANIDPFVEEYVCQRMGLKAEPVSTQVIPRDRHAEYFCTLALIASSMERIAVEI
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGQARYIRSLCIPALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERYIGPDATVALDFSLRRLNGLIENLVVYPENMLRNLNQMKGLVFSQKILLD
CCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTQAGVSREDSYRLVQRNAMKVWEQGKDFLTELLNDQEVVGALGEAKIRESFDLNYHLKH
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
VNTIFKRVFGE
HHHHHHHHHCC
>Mature Secondary Structure
MIERYSRPEMAHIWTAQNRFKIWLEIETLACEAQAELGVIPKDVVPVIREKGNFDIARID
CCCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEHH
AIEAEVKHDVIAFLTSVSEYVGPEARFIHQGMTSSDVLDTCLSVQLTQAADQLLVDLDMV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASIRKRALEHRDTVCIGRSHGIHAEPVTFGLKLATWYAEMQRNRDRLAASRETIATGAI
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGAVGTFANIDPFVEEYVCQRMGLKAEPVSTQVIPRDRHAEYFCTLALIASSMERIAVEI
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGQARYIRSLCIPALENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERYIGPDATVALDFSLRRLNGLIENLVVYPENMLRNLNQMKGLVFSQKILLD
CCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTQAGVSREDSYRLVQRNAMKVWEQGKDFLTELLNDQEVVGALGEAKIRESFDLNYHLKH
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHH
VNTIFKRVFGE
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]