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The map label for this gene is trmD [H]

Identifier: 118579528

GI number: 118579528

Start: 1143570

End: 1144316

Strand: Reverse

Name: trmD [H]

Synonym: Ppro_1095

Alternate gene names: 118579528

Gene position: 1144316-1143570 (Counterclockwise)

Preceding gene: 118579529

Following gene: 118579527

Centisome position: 28.55

GC content: 60.91

Gene sequence:

>747_bases
ATGATTTTCGACATACTGACCCTCTTTCCCGGCATGTTCCGCGGCCCCTTCGACGAGAGCATCATCAGGCGGGCCAGGGA
AAAACAGCTCATTGACATCAGCCTGCACAACATCCGTGACTATGCCCTGGATCGGCACCAGGTAACGGACGATGCTCCCT
ACGGAGGGGGCGCCGGCATGGTGATGAAGGTTGAACCGCTCTCCGCCTGCATCCAGGCCGTGAAGACTCTTCGCCCCGCA
TCCACCGTGGTGATGACCTCCCCCCAGGGGCGCCCCCTCAGTCATGGGGTTGCCCAGGAACTAAGCCGGCGTCAGGGAAT
GATTATCATCTGCGGACGCTACGAGGGGGTTGACGAACGCATCCGTGACCTGTACGTGGAAGACGACATCTCCCTGGGAG
ACTTTGTCCTCTCCGGCGGAGAAATCGCCGCCATGGCCATCGTGGACGCGGTCACGCGCCTGATTCCGGGCGCGCTGGGG
TGCGACGAATCGGCTGCGACCGACTCGTTCTGCGACGGTCTCCTGGAATATCCACAGTATACGCGGCCGCCTGAGTTCAT
GGGGTTGTCGGTGCCGGAGATACTGCTGTCGGGAAATCACGAGCTGATCAGGAAGTGGCGGCGCGCGCAATCCCTGGGGA
AGACTGCCAGATTGCGGCCAGACCTGCTTGCCAAAGTCGCGCTTAATGACGAGGACCGCAGGCTTCTTAAGGGACTGGAA
CAAGAACATGGCTCAGCCGTCCGCTAA

Upstream 100 bases:

>100_bases
GGCGCGAATACTTGATCCCGGCCATAGCCGATGTGATCCACAGTGTCGATCTGGAGATGGGCCGGATGCTGATCACCCCG
CTGGAAGGCCTGCTGGATCT

Downstream 100 bases:

>100_bases
TCTGGCTATAGCCCTGTTGCACCACCCGGTGTACAACAAGAGGCGCGAACTGGTCACCACGGCGCTGACCAATCTGGACC
TGCACGACATCGCCCGATCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIFDILTLFPGMFRGPFDESIIRRAREKQLIDISLHNIRDYALDRHQVTDDAPYGGGAGMVMKVEPLSACIQAVKTLRPA
STVVMTSPQGRPLSHGVAQELSRRQGMIIICGRYEGVDERIRDLYVEDDISLGDFVLSGGEIAAMAIVDAVTRLIPGALG
CDESAATDSFCDGLLEYPQYTRPPEFMGLSVPEILLSGNHELIRKWRRAQSLGKTARLRPDLLAKVALNDEDRRLLKGLE
QEHGSAVR

Sequences:

>Translated_248_residues
MIFDILTLFPGMFRGPFDESIIRRAREKQLIDISLHNIRDYALDRHQVTDDAPYGGGAGMVMKVEPLSACIQAVKTLRPA
STVVMTSPQGRPLSHGVAQELSRRQGMIIICGRYEGVDERIRDLYVEDDISLGDFVLSGGEIAAMAIVDAVTRLIPGALG
CDESAATDSFCDGLLEYPQYTRPPEFMGLSVPEILLSGNHELIRKWRRAQSLGKTARLRPDLLAKVALNDEDRRLLKGLE
QEHGSAVR
>Mature_248_residues
MIFDILTLFPGMFRGPFDESIIRRAREKQLIDISLHNIRDYALDRHQVTDDAPYGGGAGMVMKVEPLSACIQAVKTLRPA
STVVMTSPQGRPLSHGVAQELSRRQGMIIICGRYEGVDERIRDLYVEDDISLGDFVLSGGEIAAMAIVDAVTRLIPGALG
CDESAATDSFCDGLLEYPQYTRPPEFMGLSVPEILLSGNHELIRKWRRAQSLGKTARLRPDLLAKVALNDEDRRLLKGLE
QEHGSAVR

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=47.1311475409836, Blast_Score=227, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27447; Mature: 27447

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDILTLFPGMFRGPFDESIIRRAREKQLIDISLHNIRDYALDRHQVTDDAPYGGGAGM
CHHHHHHHCCHHHCCCCCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VMKVEPLSACIQAVKTLRPASTVVMTSPQGRPLSHGVAQELSRRQGMIIICGRYEGVDER
EEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHH
IRDLYVEDDISLGDFVLSGGEIAAMAIVDAVTRLIPGALGCDESAATDSFCDGLLEYPQY
HHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
TRPPEFMGLSVPEILLSGNHELIRKWRRAQSLGKTARLRPDLLAKVALNDEDRRLLKGLE
CCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCCHHHHHHHHHH
QEHGSAVR
HHCCCCCC
>Mature Secondary Structure
MIFDILTLFPGMFRGPFDESIIRRAREKQLIDISLHNIRDYALDRHQVTDDAPYGGGAGM
CHHHHHHHCCHHHCCCCCHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VMKVEPLSACIQAVKTLRPASTVVMTSPQGRPLSHGVAQELSRRQGMIIICGRYEGVDER
EEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHH
IRDLYVEDDISLGDFVLSGGEIAAMAIVDAVTRLIPGALGCDESAATDSFCDGLLEYPQY
HHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
TRPPEFMGLSVPEILLSGNHELIRKWRRAQSLGKTARLRPDLLAKVALNDEDRRLLKGLE
CCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCCHHHHHHHHHH
QEHGSAVR
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA