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The map label for this gene is metF [H]

Identifier: 118579522

GI number: 118579522

Start: 1138645

End: 1139517

Strand: Reverse

Name: metF [H]

Synonym: Ppro_1089

Alternate gene names: 118579522

Gene position: 1139517-1138645 (Counterclockwise)

Preceding gene: 118579524

Following gene: 118579521

Centisome position: 28.43

GC content: 64.49

Gene sequence:

>873_bases
GTGCGTATAGCCGATCTGATAGCAACCAGAAGTCCGTTCGTTTCGCTGGAATTCTTTCCCCCCAAAGAAAGGTCCGAGTG
GCCAGCGTTCTTTCAGGCCGTGGAGCAGCTGGCCCAGGTGGACCCGCTGTTCGTCTCGGTCACCTATGGAGCGGGAGGAA
GCACCCAGAGCGACACCCTTGAGATCGTCAGCCGCCTCACCCGCAATCTGGGCCTGGAGGCCATGGCGCATCTGACCTGT
GTCGGCGCGACCCCGGAGGCGATCCTGGAATTCCTGAACCAGCTGTCGGCAGCCGGCGTGCGCAACGTGCTGGCCCTGCG
GGGAGACCCCCCCAAGGACGCTCCCGATAACTTCCTCTCCTGCCACAGCCTGCAGTACGCCTCCGACCTGGTCGCCTACA
TCCGCTCGGTGCACCCGGAACTTGGCCTGGGCGTGGCCGGCTACCCCGAGACCCACCCCCAGGCCAAGAGCCCCCAAAGC
GACCTTGACTTTCTCAGGCTCAAGCTGGACAATGGCGGCGATTTCGTCATCACCCAGCTCTTCTTCGACAACACGAGCTA
CTTCGATTTCGTCCGCAGGGCGCGCGACCAGGGAATCGAGAAGCCGATCATCCCCGGCATCATCCCGGTGGTCAGCCTGA
AGGTCATTCAGCGCATCGTATCGCTGTGCGGGGCGACCATCCCTGCCCGCTTTCTGGCTGAACTGGAGCAGGCGGACCGC
GACGGCGGTGCCGCTGCCGTGCGTGAAGCGGGTGTGGCCCATGCCCGCCGCCAGGCTGAGGAGCTTCTGGCCGGCGGTGC
GCCGGGCATACACATTTACACCCTGAACCGCGCCGATGTCGTCCTGGAGCTGACCAGGGGGCTTATCCCCTGA

Upstream 100 bases:

>100_bases
GCCCGGGAAGCCCGATGCCCCGGCAGGATGAGTCGATCCGCCATCCGGCTTCGCACCACACGACGCCGCACCGAATCATG
CAACAAGCAACGGAGGAACC

Downstream 100 bases:

>100_bases
TCATCCCGGTCGAGCCTGATCGTCCGCCGGGATAAAGAACTGTTGACAGACGGCTTGCAAAGCCGTCAAATGCACCAGCG
GATCATGTCAATTTCAGAGG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIADLIATRSPFVSLEFFPPKERSEWPAFFQAVEQLAQVDPLFVSVTYGAGGSTQSDTLEIVSRLTRNLGLEAMAHLTC
VGATPEAILEFLNQLSAAGVRNVLALRGDPPKDAPDNFLSCHSLQYASDLVAYIRSVHPELGLGVAGYPETHPQAKSPQS
DLDFLRLKLDNGGDFVITQLFFDNTSYFDFVRRARDQGIEKPIIPGIIPVVSLKVIQRIVSLCGATIPARFLAELEQADR
DGGAAAVREAGVAHARRQAEELLAGGAPGIHIYTLNRADVVLELTRGLIP

Sequences:

>Translated_290_residues
MRIADLIATRSPFVSLEFFPPKERSEWPAFFQAVEQLAQVDPLFVSVTYGAGGSTQSDTLEIVSRLTRNLGLEAMAHLTC
VGATPEAILEFLNQLSAAGVRNVLALRGDPPKDAPDNFLSCHSLQYASDLVAYIRSVHPELGLGVAGYPETHPQAKSPQS
DLDFLRLKLDNGGDFVITQLFFDNTSYFDFVRRARDQGIEKPIIPGIIPVVSLKVIQRIVSLCGATIPARFLAELEQADR
DGGAAAVREAGVAHARRQAEELLAGGAPGIHIYTLNRADVVLELTRGLIP
>Mature_290_residues
MRIADLIATRSPFVSLEFFPPKERSEWPAFFQAVEQLAQVDPLFVSVTYGAGGSTQSDTLEIVSRLTRNLGLEAMAHLTC
VGATPEAILEFLNQLSAAGVRNVLALRGDPPKDAPDNFLSCHSLQYASDLVAYIRSVHPELGLGVAGYPETHPQAKSPQS
DLDFLRLKLDNGGDFVITQLFFDNTSYFDFVRRARDQGIEKPIIPGIIPVVSLKVIQRIVSLCGATIPARFLAELEQADR
DGGAAAVREAGVAHARRQAEELLAGGAPGIHIYTLNRADVVLELTRGLIP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=286, Percent_Identity=36.7132867132867, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=36.7272727272727, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI25147436, Length=294, Percent_Identity=37.4149659863946, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI25147441, Length=294, Percent_Identity=37.4149659863946, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6325234, Length=302, Percent_Identity=36.0927152317881, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321313, Length=272, Percent_Identity=33.8235294117647, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24662658, Length=198, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31487; Mature: 31487

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIADLIATRSPFVSLEFFPPKERSEWPAFFQAVEQLAQVDPLFVSVTYGAGGSTQSDTL
CCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
EIVSRLTRNLGLEAMAHLTCVGATPEAILEFLNQLSAAGVRNVLALRGDPPKDAPDNFLS
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
CHSLQYASDLVAYIRSVHPELGLGVAGYPETHPQAKSPQSDLDFLRLKLDNGGDFVITQL
HHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
FFDNTSYFDFVRRARDQGIEKPIIPGIIPVVSLKVIQRIVSLCGATIPARFLAELEQADR
ECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DGGAAAVREAGVAHARRQAEELLAGGAPGIHIYTLNRADVVLELTRGLIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure
MRIADLIATRSPFVSLEFFPPKERSEWPAFFQAVEQLAQVDPLFVSVTYGAGGSTQSDTL
CCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
EIVSRLTRNLGLEAMAHLTCVGATPEAILEFLNQLSAAGVRNVLALRGDPPKDAPDNFLS
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
CHSLQYASDLVAYIRSVHPELGLGVAGYPETHPQAKSPQSDLDFLRLKLDNGGDFVITQL
HHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
FFDNTSYFDFVRRARDQGIEKPIIPGIIPVVSLKVIQRIVSLCGATIPARFLAELEQADR
ECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DGGAAAVREAGVAHARRQAEELLAGGAPGIHIYTLNRADVVLELTRGLIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]