Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 118579485

GI number: 118579485

Start: 1102809

End: 1103420

Strand: Reverse

Name: rhtB [H]

Synonym: Ppro_1052

Alternate gene names: 118579485

Gene position: 1103420-1102809 (Counterclockwise)

Preceding gene: 118579486

Following gene: 118579484

Centisome position: 27.53

GC content: 65.03

Gene sequence:

>612_bases
ATGATCAGCGTGCAGTTTCTGCTCACCTCCCTCGTCGTGGTACTCATCCCGGGTACCGGCGTCATCTACACCGTTTCCAC
CGGCATCACCCGGGGACGAAGAGCCGCTCTGTACGCCTCCCTCGGCTGCACCGCCGGGATCGTCCCCCACCTGCTGGCAA
CCATCCTCGGCCTGACAGCCCTCATGCATACCAGCGCCCTGGCCTTCCAGACCCTCAGGTACGCCGGGGTCGCCTATCTC
TTGTACATCGCCTGGGCCACCTGGAAGGACCGGTCGGCATTCGCCCTGGACGACGCCCCGCGACAAAACTCCGCCCTATC
CCTGGTGTTCAGGGGAGTGCTGCTGAACATCCTCAACCCCAAGCTGACGATCTTCTTCCTCGCCTTCCTCCCCCAGTTCG
TCACCCCCGTCGCCAGCCCCCTGCCCCAGCTGCTGCTCCTGAGCGGCGTGTTCATGGCCATGACCTTCGCGGTGTTCGTG
GTCTATGGGCTCCTCGCCCATCAGTTCCGCGGGGTGGTGATCGAGTCGGCACGGGTCCAGGCCTGGCTGCGCCGGAGCTT
TGCCGCCGCCTTCGCCGGCCTGGGACTCCACCTGGCTTTTTCCGAGAGATAA

Upstream 100 bases:

>100_bases
AGCCTTTAAGTCTCCCCTGATAAGGGGAGGTTTGGAGGGGTTTGGTGCGAAAAAATAAGCGTTACAAGGTACTATCCCCC
TCATCAACCGGAGGCCGCCC

Downstream 100 bases:

>100_bases
GGGACGCAGCATAGGTGAATCCCCCGCATCACCCCCAGGCAACCCCTCTCCTAGTATCCTTCCTTCATGGCGCGAATCGC
ATGAGTCATTGCGACAGAAA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MISVQFLLTSLVVVLIPGTGVIYTVSTGITRGRRAALYASLGCTAGIVPHLLATILGLTALMHTSALAFQTLRYAGVAYL
LYIAWATWKDRSAFALDDAPRQNSALSLVFRGVLLNILNPKLTIFFLAFLPQFVTPVASPLPQLLLLSGVFMAMTFAVFV
VYGLLAHQFRGVVIESARVQAWLRRSFAAAFAGLGLHLAFSER

Sequences:

>Translated_203_residues
MISVQFLLTSLVVVLIPGTGVIYTVSTGITRGRRAALYASLGCTAGIVPHLLATILGLTALMHTSALAFQTLRYAGVAYL
LYIAWATWKDRSAFALDDAPRQNSALSLVFRGVLLNILNPKLTIFFLAFLPQFVTPVASPLPQLLLLSGVFMAMTFAVFV
VYGLLAHQFRGVVIESARVQAWLRRSFAAAFAGLGLHLAFSER
>Mature_203_residues
MISVQFLLTSLVVVLIPGTGVIYTVSTGITRGRRAALYASLGCTAGIVPHLLATILGLTALMHTSALAFQTLRYAGVAYL
LYIAWATWKDRSAFALDDAPRQNSALSLVFRGVLLNILNPKLTIFFLAFLPQFVTPVASPLPQLLLLSGVFMAMTFAVFV
VYGLLAHQFRGVVIESARVQAWLRRSFAAAFAGLGLHLAFSER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=194, Percent_Identity=30.9278350515464, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1788099, Length=133, Percent_Identity=27.0676691729323, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22002; Mature: 22002

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVQFLLTSLVVVLIPGTGVIYTVSTGITRGRRAALYASLGCTAGIVPHLLATILGLTA
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LMHTSALAFQTLRYAGVAYLLYIAWATWKDRSAFALDDAPRQNSALSLVFRGVLLNILNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCH
KLTIFFLAFLPQFVTPVASPLPQLLLLSGVFMAMTFAVFVVYGLLAHQFRGVVIESARVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLRRSFAAAFAGLGLHLAFSER
HHHHHHHHHHHHHCCEEEEECCC
>Mature Secondary Structure
MISVQFLLTSLVVVLIPGTGVIYTVSTGITRGRRAALYASLGCTAGIVPHLLATILGLTA
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LMHTSALAFQTLRYAGVAYLLYIAWATWKDRSAFALDDAPRQNSALSLVFRGVLLNILNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHCH
KLTIFFLAFLPQFVTPVASPLPQLLLLSGVFMAMTFAVFVVYGLLAHQFRGVVIESARVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLRRSFAAAFAGLGLHLAFSER
HHHHHHHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]