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The map label for this gene is lipA

Identifier: 118579463

GI number: 118579463

Start: 1079354

End: 1080298

Strand: Direct

Name: lipA

Synonym: Ppro_1029

Alternate gene names: 118579463

Gene position: 1079354-1080298 (Clockwise)

Preceding gene: 118579462

Following gene: 118579464

Centisome position: 26.93

GC content: 60.95

Gene sequence:

>945_bases
ATGGACGTACAACCAACAACGATGCGGGGAGCGGTGAAAACCGCCCATCTGGGAACGGATGTATCCCTTGCCACCAAGCC
GTTGCCCAAGCCGGAGTGGCTGCGGGGAAAATCGGCCAGCACGCCGGATGTGGAGAGGTTGGTGAGGATCCTTCGGGACA
ACCGGCTGCACACGGTCTGCGAAGAGGCATCCTGCCCCAACCTGGGCGAATGCTTCCGCAAGGGGACCGCGACCTTCATG
ATCATGGGGGATGTGTGCACACGGCACTGCCCGTTCTGCAACGTGGCCCATGGCTCACCCCATGAGCTGGCGGCCGATGA
GCCGGTCAATCTGGCCCGGGCCGTTGAACTCTTGAAATTGAGCTACGTGGTCATCACCTCGGTGACCAGGGATGATCTCC
CTGACGGTGGCGCCGGCCATTACGGAGCCTGTGTCCGGGCCCTGCGGGACCTGAAGCGGAGCCTGAAGGTTGAAATACTG
ACTCCCGATTTTCGCGGTGCTGTGGCCGTGGCATTCGAAGAGCTGCGCATGAATCTCCCCGATGTGTTCAACCATAACCT
GGAAACGGTGCCGCGCCTGTACCCCAGGGTGCGCCCCCAGGCCGATTATCATGGCTCTTTGGATCTGTTGCTCCGCTTCA
GGGAACAGTTCGATCATGTCCCCACCAAATCCGGGCTGATGCTTGGCCTGGGTGAAACGGAGCAGGAAGTGCGGGATGTC
ATGGAAGAACTGCGCCGCCACCGCTGCGACATGCTGACCCTGGGGCAGTACATGCGACCCAGTCCGCATCATCTGCCGGT
GGAGCGCTACGTCACCCCCGACGAATTCGAGCGTTACCGGCAGTTCGGGCTCTCCATCGGCTTTTCCCATGTGGAATCAG
GCCCCATGGTGCGCTCGTCCTACCATGCGGACATGCAGGCACGGGAACTTATGCTGGTAAACTGA

Upstream 100 bases:

>100_bases
CTCCCACAGGGAGCCGCAACGCCCTGGCAGCTGCTTTGTTGCTTTGTGTGAGTCAATGGTTTCCATGTCAGCATGGGGGA
AATTCGGGGAGATGATCTCT

Downstream 100 bases:

>100_bases
TAGACACGAGGCTCGCGTGACGTTCACGTCATCCCCTCAGGGGGGGCAACAGGGCCGGGACGTTTGCGCGTGGCTTAAAC
CACGAAAAACAAGAGGTGCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MDVQPTTMRGAVKTAHLGTDVSLATKPLPKPEWLRGKSASTPDVERLVRILRDNRLHTVCEEASCPNLGECFRKGTATFM
IMGDVCTRHCPFCNVAHGSPHELAADEPVNLARAVELLKLSYVVITSVTRDDLPDGGAGHYGACVRALRDLKRSLKVEIL
TPDFRGAVAVAFEELRMNLPDVFNHNLETVPRLYPRVRPQADYHGSLDLLLRFREQFDHVPTKSGLMLGLGETEQEVRDV
MEELRRHRCDMLTLGQYMRPSPHHLPVERYVTPDEFERYRQFGLSIGFSHVESGPMVRSSYHADMQARELMLVN

Sequences:

>Translated_314_residues
MDVQPTTMRGAVKTAHLGTDVSLATKPLPKPEWLRGKSASTPDVERLVRILRDNRLHTVCEEASCPNLGECFRKGTATFM
IMGDVCTRHCPFCNVAHGSPHELAADEPVNLARAVELLKLSYVVITSVTRDDLPDGGAGHYGACVRALRDLKRSLKVEIL
TPDFRGAVAVAFEELRMNLPDVFNHNLETVPRLYPRVRPQADYHGSLDLLLRFREQFDHVPTKSGLMLGLGETEQEVRDV
MEELRRHRCDMLTLGQYMRPSPHHLPVERYVTPDEFERYRQFGLSIGFSHVESGPMVRSSYHADMQARELMLVN
>Mature_314_residues
MDVQPTTMRGAVKTAHLGTDVSLATKPLPKPEWLRGKSASTPDVERLVRILRDNRLHTVCEEASCPNLGECFRKGTATFM
IMGDVCTRHCPFCNVAHGSPHELAADEPVNLARAVELLKLSYVVITSVTRDDLPDGGAGHYGACVRALRDLKRSLKVEIL
TPDFRGAVAVAFEELRMNLPDVFNHNLETVPRLYPRVRPQADYHGSLDLLLRFREQFDHVPTKSGLMLGLGETEQEVRDV
MEELRRHRCDMLTLGQYMRPSPHHLPVERYVTPDEFERYRQFGLSIGFSHVESGPMVRSSYHADMQARELMLVN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=280, Percent_Identity=46.0714285714286, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=45.9016393442623, Blast_Score=204, Evalue=8e-53,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=57.4468085106383, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32564533, Length=283, Percent_Identity=43.8162544169611, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=280, Percent_Identity=42.1428571428571, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI221513272, Length=280, Percent_Identity=43.5714285714286, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PELPD (A1AMT5)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900713.1
- STRING:   A1AMT5
- GeneID:   4572769
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_1029
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   A1AMT5
- ProtClustDB:   PRK12928
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35484; Mature: 35484

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVQPTTMRGAVKTAHLGTDVSLATKPLPKPEWLRGKSASTPDVERLVRILRDNRLHTVC
CCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCHHHHH
EEASCPNLGECFRKGTATFMIMGDVCTRHCPFCNVAHGSPHELAADEPVNLARAVELLKL
HHCCCCCHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SYVVITSVTRDDLPDGGAGHYGACVRALRDLKRSLKVEILTPDFRGAVAVAFEELRMNLP
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCC
DVFNHNLETVPRLYPRVRPQADYHGSLDLLLRFREQFDHVPTKSGLMLGLGETEQEVRDV
HHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
MEELRRHRCDMLTLGQYMRPSPHHLPVERYVTPDEFERYRQFGLSIGFSHVESGPMVRSS
HHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCHHHHCCCCCCHHH
YHADMQARELMLVN
HHCCHHHCCEEECC
>Mature Secondary Structure
MDVQPTTMRGAVKTAHLGTDVSLATKPLPKPEWLRGKSASTPDVERLVRILRDNRLHTVC
CCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCHHHHH
EEASCPNLGECFRKGTATFMIMGDVCTRHCPFCNVAHGSPHELAADEPVNLARAVELLKL
HHCCCCCHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SYVVITSVTRDDLPDGGAGHYGACVRALRDLKRSLKVEILTPDFRGAVAVAFEELRMNLP
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCC
DVFNHNLETVPRLYPRVRPQADYHGSLDLLLRFREQFDHVPTKSGLMLGLGETEQEVRDV
HHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHH
MEELRRHRCDMLTLGQYMRPSPHHLPVERYVTPDEFERYRQFGLSIGFSHVESGPMVRSS
HHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCHHHHCCCCCCHHH
YHADMQARELMLVN
HHCCHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA