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The map label for this gene is pilT [H]

Identifier: 118579252

GI number: 118579252

Start: 859386

End: 860471

Strand: Direct

Name: pilT [H]

Synonym: Ppro_0814

Alternate gene names: 118579252

Gene position: 859386-860471 (Clockwise)

Preceding gene: 118579251

Following gene: 118579253

Centisome position: 21.44

GC content: 62.71

Gene sequence:

>1086_bases
ATGACGAACTTCAGGGACGGATTGGAGGTTCTGCTGCGGACCTGTGTTGAACTGGGCGCTTCAGATATGCATCTCTCCGG
CGGGGAGCGGGTGCGCTTCAGGGTCAACGGGCTGCTGCAAGCCCATGGAAACGAGCTGTTTTCCTCCGATGCCGTTGAGG
CCATGGCAACGGCGATCATGAACGAACACCAGCGGAAGGAGTTTTTTACCAGGCTGACGGCCGATATCGGCTACTCCTCC
TCCGATGGCGAGCGTTACAGGGTCAACTGTTTCCGGGAAATGTGCAAGCCGGCCATGACCGTGCGCCACCTGGACCAGCG
GCTGCGCTCTCTTGAGGAGCTGGGGCTTCCGAGCCGGCTGCGGGAACTGGCCTATCTCCATTCGGGCCTGGTGCTGGTGA
CCGGCGCCACCGGCAGCGGTAAATCGTCCACGCTGGCAATGCTGCTGAACGAGATCAACCGCACCCGCGACTGCCATATC
CTGACGGTGGAGGATCCCGTGGAATTCATCCATACCAGCCAGCGGAGCCTGGTGCATCACCGCGAGGTGCACACCGACGT
TCCCAGCTATGCCGAGGCGGTCCGCGCCGCCATGCGCGAGGATCCTGACGTTATCATGGTCGGCGAGATGCGGGACCTGG
AGACGATGCAGGCGTCTCTCGCCGCCGCGGAAACCGGTCATCTGGTGTTTTCAACCCTCCATACCGGCGAGGCGGTGGGC
GCAATCGAGCGCTTCATCGGCTATTTTTCCGGCGAGGAGCAGGCCGTGGCCCGGCACCGCATCGCGATGGTGCTGCGGGC
CATTGTCGCCCAGCGGCTGGTCATAGGGCTGGACGGCAGGAAGAGGGTGCCTGCGGTGGAGTTGCTGATGGTCAACACGG
CCGCGGCCAACCTGATCCAGACCTCCAGGACCAGACAGCTCTACTCCATGATGGAGACCGGCGCCAAGGACGGGATGTGG
ACCCTGGACCAGGACCTGGCCCGTCTGGTCAAGTCCAATAAAATTAGTCGGGAAACGGCCCTGACCTACTGCAACAACCG
GGAAGGTTTCGACCGGCTGGTCAGTGTCGCCGTGGAGCGGGAGTGA

Upstream 100 bases:

>100_bases
AGGTGAAAATGCACTCCCGGCATCTGGATGAAGAACAACGGAAAAGCTCGACGCCTCTGCCATCCGGCTTAATCAGTGTG
TCCATCGAGATGTCTATCTG

Downstream 100 bases:

>100_bases
AGAGCATATGCCGGTAAACGAAACCATTCTGATCGAGGCCGCCATCAAGACCGGCCTGCTCAGGGAAGCCGATATCAAGA
CGTTGCGGTTGCAGGCCAAA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTNFRDGLEVLLRTCVELGASDMHLSGGERVRFRVNGLLQAHGNELFSSDAVEAMATAIMNEHQRKEFFTRLTADIGYSS
SDGERYRVNCFREMCKPAMTVRHLDQRLRSLEELGLPSRLRELAYLHSGLVLVTGATGSGKSSTLAMLLNEINRTRDCHI
LTVEDPVEFIHTSQRSLVHHREVHTDVPSYAEAVRAAMREDPDVIMVGEMRDLETMQASLAAAETGHLVFSTLHTGEAVG
AIERFIGYFSGEEQAVARHRIAMVLRAIVAQRLVIGLDGRKRVPAVELLMVNTAAANLIQTSRTRQLYSMMETGAKDGMW
TLDQDLARLVKSNKISRETALTYCNNREGFDRLVSVAVERE

Sequences:

>Translated_361_residues
MTNFRDGLEVLLRTCVELGASDMHLSGGERVRFRVNGLLQAHGNELFSSDAVEAMATAIMNEHQRKEFFTRLTADIGYSS
SDGERYRVNCFREMCKPAMTVRHLDQRLRSLEELGLPSRLRELAYLHSGLVLVTGATGSGKSSTLAMLLNEINRTRDCHI
LTVEDPVEFIHTSQRSLVHHREVHTDVPSYAEAVRAAMREDPDVIMVGEMRDLETMQASLAAAETGHLVFSTLHTGEAVG
AIERFIGYFSGEEQAVARHRIAMVLRAIVAQRLVIGLDGRKRVPAVELLMVNTAAANLIQTSRTRQLYSMMETGAKDGMW
TLDQDLARLVKSNKISRETALTYCNNREGFDRLVSVAVERE
>Mature_360_residues
TNFRDGLEVLLRTCVELGASDMHLSGGERVRFRVNGLLQAHGNELFSSDAVEAMATAIMNEHQRKEFFTRLTADIGYSSS
DGERYRVNCFREMCKPAMTVRHLDQRLRSLEELGLPSRLRELAYLHSGLVLVTGATGSGKSSTLAMLLNEINRTRDCHIL
TVEDPVEFIHTSQRSLVHHREVHTDVPSYAEAVRAAMREDPDVIMVGEMRDLETMQASLAAAETGHLVFSTLHTGEAVGA
IERFIGYFSGEEQAVARHRIAMVLRAIVAQRLVIGLDGRKRVPAVELLMVNTAAANLIQTSRTRQLYSMMETGAKDGMWT
LDQDLARLVKSNKISRETALTYCNNREGFDRLVSVAVERE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=317, Percent_Identity=39.1167192429022, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789723, Length=282, Percent_Identity=35.8156028368794, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786296, Length=293, Percent_Identity=34.1296928327645, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40350; Mature: 40219

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFRDGLEVLLRTCVELGASDMHLSGGERVRFRVNGLLQAHGNELFSSDAVEAMATAIM
CCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHH
NEHQRKEFFTRLTADIGYSSSDGERYRVNCFREMCKPAMTVRHLDQRLRSLEELGLPSRL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RELAYLHSGLVLVTGATGSGKSSTLAMLLNEINRTRDCHILTVEDPVEFIHTSQRSLVHH
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHH
REVHTDVPSYAEAVRAAMREDPDVIMVGEMRDLETMQASLAAAETGHLVFSTLHTGEAVG
HHHHCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHCCHHHH
AIERFIGYFSGEEQAVARHRIAMVLRAIVAQRLVIGLDGRKRVPAVELLMVNTAAANLIQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHHH
TSRTRQLYSMMETGAKDGMWTLDQDLARLVKSNKISRETALTYCNNREGFDRLVSVAVER
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
TNFRDGLEVLLRTCVELGASDMHLSGGERVRFRVNGLLQAHGNELFSSDAVEAMATAIM
CCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHH
NEHQRKEFFTRLTADIGYSSSDGERYRVNCFREMCKPAMTVRHLDQRLRSLEELGLPSRL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RELAYLHSGLVLVTGATGSGKSSTLAMLLNEINRTRDCHILTVEDPVEFIHTSQRSLVHH
HHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHH
REVHTDVPSYAEAVRAAMREDPDVIMVGEMRDLETMQASLAAAETGHLVFSTLHTGEAVG
HHHHCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHCCHHHH
AIERFIGYFSGEEQAVARHRIAMVLRAIVAQRLVIGLDGRKRVPAVELLMVNTAAANLIQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHHH
TSRTRQLYSMMETGAKDGMWTLDQDLARLVKSNKISRETALTYCNNREGFDRLVSVAVER
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]