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The map label for this gene is dosC [H]

Identifier: 118579233

GI number: 118579233

Start: 842249

End: 842998

Strand: Direct

Name: dosC [H]

Synonym: Ppro_0795

Alternate gene names: 118579233

Gene position: 842249-842998 (Clockwise)

Preceding gene: 118579232

Following gene: 118579234

Centisome position: 21.01

GC content: 61.47

Gene sequence:

>750_bases
ATGTCAGAAATATACGTTGATGAGATGTTCGTGGGGGAGGCGGCAGAAATTATGTCCATCAGCGACGTATTCCCCGAATA
CCGGCAGTTGCGCACCATGGGGTTCCGTGAGGGAAGGTTGGTGGATCTTCTGCACTTCGATCCGCTGATTACCAGGAAGC
TGGTCTTGCGGGTGGAGGGCATGCGCCTGGCAATGGGGGCGTCCTGCGCCGCTCACATAAGGGTCAGGCCGCTGAAGAGC
GCCTATCTTCACCTGCGGGACATGGCCCACTACGACAACCTGACCGGCTGCTTCAACCGCCATGCAGCCGGGACCGTCGT
GCGCCAGGAGGTGGAACGCTTTTCCAGTCTGGGGCTCCCTCTGGCGCTGCTCATGGCCGACCTGGACCACTTCAAGCGCA
TCAACGACACCTTTGGTCACACGTCGGGTGACGATGTTCTCAAGTCCTTCGCCTCGCTCATCCGTCAGGGGCTGCGGCGC
TCGGACCTGTTCTGTCGCTGGGGGGGGGAGGAGTTCCTGATCCTGCTCAGGGGGACGCTGAAGGAAGAGGCGCTGAAGAT
CGCCGAACGTTTCCGGGAGCGGATCGCCTCGGCGCTATTTCCGCCGTTCAACGAACGCGGAGCCGTTACGGTCAGCATCG
GCTGCGCCTGTCTTCCTCCCGGCAGACCGTTGGAGCGACTGATCGTCGATGCCGATTGTGCCTTGTACCGCGCCAAGCAG
GGGGGGAGGAACAGGGTGTGCCTATGTTGA

Upstream 100 bases:

>100_bases
GCGGTACGAAGACGAAAGAATCAAAATAGTTTTTCTTCGCGTGCCTCGCGGCTTCGCGTGAACAACCGCTTTTTCAGGAC
ACGCGGGAAGAGAGAATACC

Downstream 100 bases:

>100_bases
ATATGGTACGGGCGACAACGTCGCTTTTTGTTATGAAACGCAACCAAAAGAAGCGCATGCGGAACAGGGGGGAAAGCCTC
GACAGTTTCACGTCGCTGCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSEIYVDEMFVGEAAEIMSISDVFPEYRQLRTMGFREGRLVDLLHFDPLITRKLVLRVEGMRLAMGASCAAHIRVRPLKS
AYLHLRDMAHYDNLTGCFNRHAAGTVVRQEVERFSSLGLPLALLMADLDHFKRINDTFGHTSGDDVLKSFASLIRQGLRR
SDLFCRWGGEEFLILLRGTLKEEALKIAERFRERIASALFPPFNERGAVTVSIGCACLPPGRPLERLIVDADCALYRAKQ
GGRNRVCLC

Sequences:

>Translated_249_residues
MSEIYVDEMFVGEAAEIMSISDVFPEYRQLRTMGFREGRLVDLLHFDPLITRKLVLRVEGMRLAMGASCAAHIRVRPLKS
AYLHLRDMAHYDNLTGCFNRHAAGTVVRQEVERFSSLGLPLALLMADLDHFKRINDTFGHTSGDDVLKSFASLIRQGLRR
SDLFCRWGGEEFLILLRGTLKEEALKIAERFRERIASALFPPFNERGAVTVSIGCACLPPGRPLERLIVDADCALYRAKQ
GGRNRVCLC
>Mature_248_residues
SEIYVDEMFVGEAAEIMSISDVFPEYRQLRTMGFREGRLVDLLHFDPLITRKLVLRVEGMRLAMGASCAAHIRVRPLKSA
YLHLRDMAHYDNLTGCFNRHAAGTVVRQEVERFSSLGLPLALLMADLDHFKRINDTFGHTSGDDVLKSFASLIRQGLRRS
DLFCRWGGEEFLILLRGTLKEEALKIAERFRERIASALFPPFNERGAVTVSIGCACLPPGRPLERLIVDADCALYRAKQG
GRNRVCLC

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=40.8805031446541, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=38.2352941176471, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=38.7878787878788, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=36.1963190184049, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=36.969696969697, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=127, Percent_Identity=36.2204724409449, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=32.0987654320988, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=34.3195266272189, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=30.5194805194805, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=34.1614906832298, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=32.5301204819277, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 28117; Mature: 27986

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIYVDEMFVGEAAEIMSISDVFPEYRQLRTMGFREGRLVDLLHFDPLITRKLVLRVEG
CCCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHCC
MRLAMGASCAAHIRVRPLKSAYLHLRDMAHYDNLTGCFNRHAAGTVVRQEVERFSSLGLP
CCEECCCCCCHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
LALLMADLDHFKRINDTFGHTSGDDVLKSFASLIRQGLRRSDLFCRWGGEEFLILLRGTL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHH
KEEALKIAERFRERIASALFPPFNERGAVTVSIGCACLPPGRPLERLIVDADCALYRAKQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHC
GGRNRVCLC
CCCCCCCCC
>Mature Secondary Structure 
SEIYVDEMFVGEAAEIMSISDVFPEYRQLRTMGFREGRLVDLLHFDPLITRKLVLRVEG
CCEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHCC
MRLAMGASCAAHIRVRPLKSAYLHLRDMAHYDNLTGCFNRHAAGTVVRQEVERFSSLGLP
CCEECCCCCCHHEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
LALLMADLDHFKRINDTFGHTSGDDVLKSFASLIRQGLRRSDLFCRWGGEEFLILLRGTL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEHHHH
KEEALKIAERFRERIASALFPPFNERGAVTVSIGCACLPPGRPLERLIVDADCALYRAKQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEECCCCCCHHHHHHHHHHHHHHHHC
GGRNRVCLC
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]