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The map label for this gene is fumB [H]

Identifier: 118579187

GI number: 118579187

Start: 789222

End: 790847

Strand: Direct

Name: fumB [H]

Synonym: Ppro_0749

Alternate gene names: 118579187

Gene position: 789222-790847 (Clockwise)

Preceding gene: 118579184

Following gene: 118579188

Centisome position: 19.69

GC content: 59.16

Gene sequence:

>1626_bases
ATGGCAACACCCGAATTCCAGTACCAGGATCCTTTTCCACTCGCTAAAGACGAGACGAAGTATCGCAAGATCGAGGGCTC
GGAGAAATATGTAACCGTCGAGCAGTTCGCCGGCAAGGATGTGGTGCGGGTCTGCCCCGAGGCGCTCACCGTACTGGCCA
ACGAGGCCATGAGGGATGTCTCTTTCTTGCTGCGTCCCGCCCATAATGAGCAGGTGGCAAAGATACTGAGCGACCCCGAA
GCGTCCCAGAACGACAAGGGTGTTGCCATGGCCTTCCTGCGTAACGCCGAAATCGCGGCCCAGCTTGACCTGCCGCTCTG
CCAGGATACCGGCACCGCCACGGTCGTTGCCAAGAAGGGGCAGCAGGTCTGGACCGGCGTCAAGGACGAAGAGTACATCT
CCAAGGGGATCTACAAGACCTATACCGAGGAAAACCTGCGGTATTCCCAGACCGTTGCCCTGGATATGTACAAAGAGGTC
AACACCGGAACCAACCTGCCCGCCCAGATCGATATCATGGCAACCGATGGCGATTACTACAAATTCGTCTTCATGGCCAA
GGGGGGCGGTTCCGCCAACAAGACCATGCTCTACCAGGAAACCAAGGCCCTGCTGACCCAGGAGAAGCTGGAAAAATTCC
TGATCGAAAAGATGAAATATCTGGGCACCGCCGCCTGTCCTCCTTACCACATCGCCTTCGTCATCGGCGGCACCAGCGCC
GACGCCTGCATGAAGACCGTCAAACTGGCGACGGCCAAGGAACTGGACGGGCTTCCCACCGAGGGTAATGCCCACGGCCA
GGCCTTCCGTGACATCGCGCTGGAGAACAAGATGCTGGAAGCTGCCCAGAAACTCGGTATCGGCGCACAGTTCGGCGGCA
AATACTTCGCCCATGACGTGCGTGTTATCCGTCTCCCCCGCCACGGCGCCTCCTGCCCGGTGGGCATGGCGGTCTCCTGT
TCGGCCGATCGCAACATCAAGGCCAAGATCACCAAGGACGGTCTGTTTGTCGAGGAGATGGACCGCAACCCAGGGCGTCT
GATTCCCGATCGTTTCCGCGGCAAGCATGAGCACGGCGTCAAGATCGACCTGAACAAGCCGATGCAGGAAGTCCTGGCCG
AGTTGACCAAGCACCCGGTTTCCACACCGCTGCTTTTGACCGGAACCATTGTTGTGGGCCGCGACATCGCCCATGCCAAG
TTCAAGGAAATTCTGGACAGCGGCAAGCCGCTGCCCGACTACCTGAAGAAACACCCGATCTACTACGCCGGTCCGGCCAA
GACCCCCAAAGGCAAGGCTTCCGGCTCCTTCGGGCCCACTACCGCGGGCCGCATGGACTCCTACGTTGACCTGCTCCAGG
CAAACGGCGGCTCCATGATCATGATCGCCAAGGGTAACCGCAGCCAGCAGGTTACGGACGCCTGCAAGAAGCACGGCGGT
TTCTATCTCGGCTCCATCGGTGGCCCTGCCGCAGTTCTGGCCGAGGAGAACATCAAGAAGGTCGAGTGCATCGACTTCCC
GGAACTGGGCATGGAAGCGGTCTGGAAGATCGAAGTGGAGAACTTCCCCGCCTTCATCCTGGTGGATGACAACGGTAACG
ACTTCTTCAAGCAGCTCGGCCTGTAA

Upstream 100 bases:

>100_bases
CACTCGACAATGAGCGCGATTTTAACTAGTATGCTGCATTGACTATTCGGAAGCACCCTTTTGATGCACATCCACACTAT
CGACGAAAGGAGTTTCACTG

Downstream 100 bases:

>100_bases
TGCCTGAGTCGAGCGCCCGGTTGATCGGCAACCGGGCGCTCGGATGGTTTTATCACCCCCGTGGTGGATGGGGCAACTGC
TGTATCAAGCAAAACGGTAG

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase beta subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MATPEFQYQDPFPLAKDETKYRKIEGSEKYVTVEQFAGKDVVRVCPEALTVLANEAMRDVSFLLRPAHNEQVAKILSDPE
ASQNDKGVAMAFLRNAEIAAQLDLPLCQDTGTATVVAKKGQQVWTGVKDEEYISKGIYKTYTEENLRYSQTVALDMYKEV
NTGTNLPAQIDIMATDGDYYKFVFMAKGGGSANKTMLYQETKALLTQEKLEKFLIEKMKYLGTAACPPYHIAFVIGGTSA
DACMKTVKLATAKELDGLPTEGNAHGQAFRDIALENKMLEAAQKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITKDGLFVEEMDRNPGRLIPDRFRGKHEHGVKIDLNKPMQEVLAELTKHPVSTPLLLTGTIVVGRDIAHAK
FKEILDSGKPLPDYLKKHPIYYAGPAKTPKGKASGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDNGNDFFKQLGL

Sequences:

>Translated_541_residues
MATPEFQYQDPFPLAKDETKYRKIEGSEKYVTVEQFAGKDVVRVCPEALTVLANEAMRDVSFLLRPAHNEQVAKILSDPE
ASQNDKGVAMAFLRNAEIAAQLDLPLCQDTGTATVVAKKGQQVWTGVKDEEYISKGIYKTYTEENLRYSQTVALDMYKEV
NTGTNLPAQIDIMATDGDYYKFVFMAKGGGSANKTMLYQETKALLTQEKLEKFLIEKMKYLGTAACPPYHIAFVIGGTSA
DACMKTVKLATAKELDGLPTEGNAHGQAFRDIALENKMLEAAQKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITKDGLFVEEMDRNPGRLIPDRFRGKHEHGVKIDLNKPMQEVLAELTKHPVSTPLLLTGTIVVGRDIAHAK
FKEILDSGKPLPDYLKKHPIYYAGPAKTPKGKASGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDNGNDFFKQLGL
>Mature_540_residues
ATPEFQYQDPFPLAKDETKYRKIEGSEKYVTVEQFAGKDVVRVCPEALTVLANEAMRDVSFLLRPAHNEQVAKILSDPEA
SQNDKGVAMAFLRNAEIAAQLDLPLCQDTGTATVVAKKGQQVWTGVKDEEYISKGIYKTYTEENLRYSQTVALDMYKEVN
TGTNLPAQIDIMATDGDYYKFVFMAKGGGSANKTMLYQETKALLTQEKLEKFLIEKMKYLGTAACPPYHIAFVIGGTSAD
ACMKTVKLATAKELDGLPTEGNAHGQAFRDIALENKMLEAAQKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSCS
ADRNIKAKITKDGLFVEEMDRNPGRLIPDRFRGKHEHGVKIDLNKPMQEVLAELTKHPVSTPLLLTGTIVVGRDIAHAKF
KEILDSGKPLPDYLKKHPIYYAGPAKTPKGKASGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDNGNDFFKQLGL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=540, Percent_Identity=70.9259259259259, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=542, Percent_Identity=69.3726937269373, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789442, Length=260, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59307; Mature: 59176

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPEFQYQDPFPLAKDETKYRKIEGSEKYVTVEQFAGKDVVRVCPEALTVLANEAMRDV
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SFLLRPAHNEQVAKILSDPEASQNDKGVAMAFLRNAEIAAQLDLPLCQDTGTATVVAKKG
HHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCEECCCCCEEEEEECC
QQVWTGVKDEEYISKGIYKTYTEENLRYSQTVALDMYKEVNTGTNLPAQIDIMATDGDYY
CHHHCCCCCHHHHHHCHHHHHHHCCCCCHHEEHHHHHHHHCCCCCCCEEEEEEEECCCEE
KFVFMAKGGGSANKTMLYQETKALLTQEKLEKFLIEKMKYLGTAACPPYHIAFVIGGTSA
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DACMKTVKLATAKELDGLPTEGNAHGQAFRDIALENKMLEAAQKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITKDGLFVEEMDRNPGRLIPDRFRGKHEHGV
EEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCHHHCCCCCCCE
KIDLNKPMQEVLAELTKHPVSTPLLLTGTIVVGRDIAHAKFKEILDSGKPLPDYLKKHPI
EEECCCCHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCE
YYAGPAKTPKGKASGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDNGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C
>Mature Secondary Structure 
ATPEFQYQDPFPLAKDETKYRKIEGSEKYVTVEQFAGKDVVRVCPEALTVLANEAMRDV
CCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SFLLRPAHNEQVAKILSDPEASQNDKGVAMAFLRNAEIAAQLDLPLCQDTGTATVVAKKG
HHHHCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCEECCCCCEEEEEECC
QQVWTGVKDEEYISKGIYKTYTEENLRYSQTVALDMYKEVNTGTNLPAQIDIMATDGDYY
CHHHCCCCCHHHHHHCHHHHHHHCCCCCHHEEHHHHHHHHCCCCCCCEEEEEEEECCCEE
KFVFMAKGGGSANKTMLYQETKALLTQEKLEKFLIEKMKYLGTAACPPYHIAFVIGGTSA
EEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DACMKTVKLATAKELDGLPTEGNAHGQAFRDIALENKMLEAAQKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITKDGLFVEEMDRNPGRLIPDRFRGKHEHGV
EEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEECCCCCCCCCCHHHCCCCCCCE
KIDLNKPMQEVLAELTKHPVSTPLLLTGTIVVGRDIAHAKFKEILDSGKPLPDYLKKHPI
EEECCCCHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCE
YYAGPAKTPKGKASGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDNGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]