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The map label for this gene is nuoF2 [H]

Identifier: 118579072

GI number: 118579072

Start: 668789

End: 670072

Strand: Direct

Name: nuoF2 [H]

Synonym: Ppro_0633

Alternate gene names: 118579072

Gene position: 668789-670072 (Clockwise)

Preceding gene: 118579071

Following gene: 118579073

Centisome position: 16.69

GC content: 60.44

Gene sequence:

>1284_bases
ATGACAAGCGAGAGAATCTTCTTCAATTTCCCCGTAACCGCCGACTCCCATACGCTTAAAGCGTACCAGGGGCGTGGCGG
CTACCAGGCCCTTGAGAACGCCCTCAAGACCCTGCAGCCCATCGACGTGGAGAAGGAAGTCATGGCATCGGGTCTTCGTG
GCCGCGGCGGCGCCGGCTTCCCCACCGGCAGCAAATGGTCCTTCGTCAACAAGAAGGCCCCCGTCGTCTACCTCTGCTGC
AACGCCGACGAAGGGGAGCCGGGCACCTTCAAAGACCGCTGGATCTTCGAGCACAACTCCCACCAGCTCATCGAAGGGAT
GATCCTGGCCGCCTACGCGCTTAACGTCAGAAACGCCTTCATCTACATCAGGGGTGAATTCGACCTCTCCTTCAGAAGGC
TCATGGACGCCATGAGTGAGGCCTACAAAGCCGGCTACCTGGGCGAGAACATCCTGGGCAGCTCCTTCTCCTGCGACATC
CGGGTCATGCAGGGTGGCGGCGCCTACGTCTGCGGCGAGGAATCCAGCCTTTATACATCCATCGAAGGCTTCAAGGGCTA
CCCGCGCAACAAGCCGCCCTTCCCGGCCGTGCAGGGCCTCTACAAAGCCCCCACCGTCGTCAACAACGTGGAGACCTTGG
CCAACGTTCCCTGGATCATGACCAACGGCGCCAAGGCCTTTAGCGCCATGGGCACCGAGAAGCACCCCGGCACCAAGATC
TTCGGCGTCTCCGGCCACGTCAAAAAACCGGGCCTGTATGAACTTCCCCTGGGCTACCCCTTAAAGAAACTGATCTTCGA
GGAGTGCGGCGGAATCCTGAACGGCAAAGAGCTCAAAGCCGTCATCCCGGGCGGCTCCTCCACCCCGATTCTCTTGCCCC
AAGACGTGGAGACCGCCAACCTGGATGCCGAATGCCTCTCCACCTACCGCACCATGCTCGGTTCCGGCGGGGTCATCGTC
ATCGCCGAAGGGGTCTGCATGGTCAGACTGCTGCACACGCTAAGCCGCTTCTACGCCCACGAATCCTGCGGCCAGTGCAC
CCCCTGCCGCGAGGGAAGCGCCTGGATGAACGACATCATCAGCCGCATCGTAGCTGGCAAGGGAGTCAAGGGAGACCTGG
AGAGTCTGGAGCGGATCACCAAGGGCATCATGGGCAACACCGTCTGCGCCCTGGGCGACGCCGCCTCCATGCCGGTCATA
AACTTCATCACGAAATTCAGAGCGGAATTCGACTACTACATCGAACACGGTCGTTCCATGAACGACGGTCGTTTGGAAAT
CTGA

Upstream 100 bases:

>100_bases
GCCTGGCCTCCTGCGGCACCGCCCCGGTCATGCAGATCGACGACACCTATCACGAGCAACTGACCGAAGCAAAAATCGAT
CAGATCTTGAAGGGGCTTTC

Downstream 100 bases:

>100_bases
AGGCGCCATGATTGAACTAAAGATCGACAACCAAGTCATCGAGACGCAGGAAGGCGAAACCGTAATGGAAGCGGCGCTCA
GGCACGGCATCCATATTCCC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTSERIFFNFPVTADSHTLKAYQGRGGYQALENALKTLQPIDVEKEVMASGLRGRGGAGFPTGSKWSFVNKKAPVVYLCC
NADEGEPGTFKDRWIFEHNSHQLIEGMILAAYALNVRNAFIYIRGEFDLSFRRLMDAMSEAYKAGYLGENILGSSFSCDI
RVMQGGGAYVCGEESSLYTSIEGFKGYPRNKPPFPAVQGLYKAPTVVNNVETLANVPWIMTNGAKAFSAMGTEKHPGTKI
FGVSGHVKKPGLYELPLGYPLKKLIFEECGGILNGKELKAVIPGGSSTPILLPQDVETANLDAECLSTYRTMLGSGGVIV
IAEGVCMVRLLHTLSRFYAHESCGQCTPCREGSAWMNDIISRIVAGKGVKGDLESLERITKGIMGNTVCALGDAASMPVI
NFITKFRAEFDYYIEHGRSMNDGRLEI

Sequences:

>Translated_427_residues
MTSERIFFNFPVTADSHTLKAYQGRGGYQALENALKTLQPIDVEKEVMASGLRGRGGAGFPTGSKWSFVNKKAPVVYLCC
NADEGEPGTFKDRWIFEHNSHQLIEGMILAAYALNVRNAFIYIRGEFDLSFRRLMDAMSEAYKAGYLGENILGSSFSCDI
RVMQGGGAYVCGEESSLYTSIEGFKGYPRNKPPFPAVQGLYKAPTVVNNVETLANVPWIMTNGAKAFSAMGTEKHPGTKI
FGVSGHVKKPGLYELPLGYPLKKLIFEECGGILNGKELKAVIPGGSSTPILLPQDVETANLDAECLSTYRTMLGSGGVIV
IAEGVCMVRLLHTLSRFYAHESCGQCTPCREGSAWMNDIISRIVAGKGVKGDLESLERITKGIMGNTVCALGDAASMPVI
NFITKFRAEFDYYIEHGRSMNDGRLEI
>Mature_426_residues
TSERIFFNFPVTADSHTLKAYQGRGGYQALENALKTLQPIDVEKEVMASGLRGRGGAGFPTGSKWSFVNKKAPVVYLCCN
ADEGEPGTFKDRWIFEHNSHQLIEGMILAAYALNVRNAFIYIRGEFDLSFRRLMDAMSEAYKAGYLGENILGSSFSCDIR
VMQGGGAYVCGEESSLYTSIEGFKGYPRNKPPFPAVQGLYKAPTVVNNVETLANVPWIMTNGAKAFSAMGTEKHPGTKIF
GVSGHVKKPGLYELPLGYPLKKLIFEECGGILNGKELKAVIPGGSSTPILLPQDVETANLDAECLSTYRTMLGSGGVIVI
AEGVCMVRLLHTLSRFYAHESCGQCTPCREGSAWMNDIISRIVAGKGVKGDLESLERITKGIMGNTVCALGDAASMPVIN
FITKFRAEFDYYIEHGRSMNDGRLEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=417, Percent_Identity=47.0023980815348, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI20149568, Length=417, Percent_Identity=47.0023980815348, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI1788620, Length=401, Percent_Identity=41.1471321695761, Blast_Score=320, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI17531727, Length=414, Percent_Identity=46.6183574879227, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI19920794, Length=423, Percent_Identity=43.7352245862884, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI45550452, Length=427, Percent_Identity=43.559718969555, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24653819, Length=369, Percent_Identity=36.5853658536585, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24653817, Length=309, Percent_Identity=35.5987055016181, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46578; Mature: 46447

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSERIFFNFPVTADSHTLKAYQGRGGYQALENALKTLQPIDVEKEVMASGLRGRGGAGF
CCCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCC
PTGSKWSFVNKKAPVVYLCCNADEGEPGTFKDRWIFEHNSHQLIEGMILAAYALNVRNAF
CCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEECEEE
IYIRGEFDLSFRRLMDAMSEAYKAGYLGENILGSSFSCDIRVMQGGGAYVCGEESSLYTS
EEEEECCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCEEEECCCCCCEEE
IEGFKGYPRNKPPFPAVQGLYKAPTVVNNVETLANVPWIMTNGAKAFSAMGTEKHPGTKI
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCCEE
FGVSGHVKKPGLYELPLGYPLKKLIFEECGGILNGKELKAVIPGGSSTPILLPQDVETAN
EECCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCC
LDAECLSTYRTMLGSGGVIVIAEGVCMVRLLHTLSRFYAHESCGQCTPCREGSAWMNDII
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SRIVAGKGVKGDLESLERITKGIMGNTVCALGDAASMPVINFITKFRAEFDYYIEHGRSM
HHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NDGRLEI
CCCCCCC
>Mature Secondary Structure 
TSERIFFNFPVTADSHTLKAYQGRGGYQALENALKTLQPIDVEKEVMASGLRGRGGAGF
CCCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCC
PTGSKWSFVNKKAPVVYLCCNADEGEPGTFKDRWIFEHNSHQLIEGMILAAYALNVRNAF
CCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEECEEE
IYIRGEFDLSFRRLMDAMSEAYKAGYLGENILGSSFSCDIRVMQGGGAYVCGEESSLYTS
EEEEECCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCEEEECCCCCCEEE
IEGFKGYPRNKPPFPAVQGLYKAPTVVNNVETLANVPWIMTNGAKAFSAMGTEKHPGTKI
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCCEE
FGVSGHVKKPGLYELPLGYPLKKLIFEECGGILNGKELKAVIPGGSSTPILLPQDVETAN
EECCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCCC
LDAECLSTYRTMLGSGGVIVIAEGVCMVRLLHTLSRFYAHESCGQCTPCREGSAWMNDII
CCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SRIVAGKGVKGDLESLERITKGIMGNTVCALGDAASMPVINFITKFRAEFDYYIEHGRSM
HHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NDGRLEI
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]