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The map label for this gene is nuoE2 [H]

Identifier: 118579071

GI number: 118579071

Start: 668328

End: 668792

Strand: Direct

Name: nuoE2 [H]

Synonym: Ppro_0632

Alternate gene names: 118579071

Gene position: 668328-668792 (Clockwise)

Preceding gene: 118579070

Following gene: 118579072

Centisome position: 16.67

GC content: 58.06

Gene sequence:

>465_bases
ATGAGCTGTATAGAAGAGAAATTCGCCCAATTGCGCGCCACCTACCCCAAGGAGCTGAACTCCTCCCTGGTCATGCCTTT
CTTAAGGATCATGCTGGACGAGAAGAAGAGCCTTGGCGAGAGCGACGCCGTCTTCATCGCCAACTACCTGGGTCTTCCGG
CCATGCAGGTCAAAGAAGCGCTCACCTGGTACACCATGTTCTACCGCCACCCGGTGGGCACGCACGTCATCAAGGTCTGC
CGCAACATCGCCTGCTCCCTCATGGGCGCCGAGCGGATCATCGACCACCTCTCCCAGAAACTGGGTATCAAACCGGGCGA
GACCACCGCTGACGGCCGTTTCACCCTGCTTCTCGTCGAATGCCTGGCCTCCTGCGGCACCGCCCCGGTCATGCAGATCG
ACGACACCTATCACGAGCAACTGACCGAAGCAAAAATCGATCAGATCTTGAAGGGGCTTTCATGA

Upstream 100 bases:

>100_bases
CGCACATGGGTGGCATGCACACCCTGCTGGAGGGATATCAAGTCGCCGACGTCATCGCCACCTTCGGCTCCATGAACATG
ATCGGCGGGGAGTGTGACCG

Downstream 100 bases:

>100_bases
CAAGCGAGAGAATCTTCTTCAATTTCCCCGTAACCGCCGACTCCCATACGCTTAAAGCGTACCAGGGGCGTGGCGGCTAC
CAGGCCCTTGAGAACGCCCT

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSCIEEKFAQLRATYPKELNSSLVMPFLRIMLDEKKSLGESDAVFIANYLGLPAMQVKEALTWYTMFYRHPVGTHVIKVC
RNIACSLMGAERIIDHLSQKLGIKPGETTADGRFTLLLVECLASCGTAPVMQIDDTYHEQLTEAKIDQILKGLS

Sequences:

>Translated_154_residues
MSCIEEKFAQLRATYPKELNSSLVMPFLRIMLDEKKSLGESDAVFIANYLGLPAMQVKEALTWYTMFYRHPVGTHVIKVC
RNIACSLMGAERIIDHLSQKLGIKPGETTADGRFTLLLVECLASCGTAPVMQIDDTYHEQLTEAKIDQILKGLS
>Mature_153_residues
SCIEEKFAQLRATYPKELNSSLVMPFLRIMLDEKKSLGESDAVFIANYLGLPAMQVKEALTWYTMFYRHPVGTHVIKVCR
NIACSLMGAERIIDHLSQKLGIKPGETTADGRFTLLLVECLASCGTAPVMQIDDTYHEQLTEAKIDQILKGLS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=151, Percent_Identity=39.0728476821192, Blast_Score=111, Evalue=3e-25,
Organism=Escherichia coli, GI1788621, Length=124, Percent_Identity=40.3225806451613, Blast_Score=101, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17561328, Length=149, Percent_Identity=39.5973154362416, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI18859877, Length=139, Percent_Identity=38.1294964028777, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21355353, Length=149, Percent_Identity=30.8724832214765, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17226; Mature: 17095

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCIEEKFAQLRATYPKELNSSLVMPFLRIMLDEKKSLGESDAVFIANYLGLPAMQVKEA
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
LTWYTMFYRHPVGTHVIKVCRNIACSLMGAERIIDHLSQKLGIKPGETTADGRFTLLLVE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CLASCGTAPVMQIDDTYHEQLTEAKIDQILKGLS
HHHHCCCCCCEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SCIEEKFAQLRATYPKELNSSLVMPFLRIMLDEKKSLGESDAVFIANYLGLPAMQVKEA
CHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
LTWYTMFYRHPVGTHVIKVCRNIACSLMGAERIIDHLSQKLGIKPGETTADGRFTLLLVE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CLASCGTAPVMQIDDTYHEQLTEAKIDQILKGLS
HHHHCCCCCCEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]