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The map label for this gene is nuoB1

Identifier: 118579068

GI number: 118579068

Start: 665956

End: 666447

Strand: Direct

Name: nuoB1

Synonym: Ppro_0629

Alternate gene names: 118579068

Gene position: 665956-666447 (Clockwise)

Preceding gene: 118579067

Following gene: 118579069

Centisome position: 16.62

GC content: 58.94

Gene sequence:

>492_bases
ATGGCGATGATGGAGTATCTGACAACCCGTAAGGACGAACTGATCGGCTGGGTCCGCAAGTTCAGTATTTTTCCCTATCC
CTTCGTCACCGCCTGCTGCGGCATGGAATTCATGGCCGTTGCCTCCACCCTGTACGACACCGACCGTTTCGGTGCCGCGC
TTCCCCGCTTTACCCCCCGTCAGTCCGACCTTTTGATGGTAGTCGGCACCATCACCCACAAGGAAGCCCCGGTCATTAAA
AGAGTCTACGACCAGATGTGCGATCCCAAATGGGTCATGGCGTTTGGCGCCTGCGCCACCAGTGGTGGTGTCTATCGCAA
CTACACCGTGCTTCAGGGTGTGGACCGGATCATCCCGGTGGATATCTACATCCCCGGCTGCCCGCCGCGCCCCGAGATGG
TCATCGACGCCATCATGAAACTGCAGGACAAGATAGCCGGCGAGCGCCACCCGATCTTCCCGTACGAGAAACAGAACCCG
GTCCCGGTCTGA

Upstream 100 bases:

>100_bases
GCAACCGCGACATGGCCACGCTCTCGTTGTTCTCGTTTCTGTTTTTCATGGGCGTGCTTTCTTTGGCATTCATCTATATC
TGGAAGGAAGGTGGTCTTGA

Downstream 100 bases:

>100_bases
TTCCGGGAAGCGAGTAACATTATCTCACACGAAGCCACGAAGAAAGCAAACACGCGGTTAGAGCCTTCTGTTTGTTCGTC
AGGTTTTCTTCGTGAACTTC

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MAMMEYLTTRKDELIGWVRKFSIFPYPFVTACCGMEFMAVASTLYDTDRFGAALPRFTPRQSDLLMVVGTITHKEAPVIK
RVYDQMCDPKWVMAFGACATSGGVYRNYTVLQGVDRIIPVDIYIPGCPPRPEMVIDAIMKLQDKIAGERHPIFPYEKQNP
VPV

Sequences:

>Translated_163_residues
MAMMEYLTTRKDELIGWVRKFSIFPYPFVTACCGMEFMAVASTLYDTDRFGAALPRFTPRQSDLLMVVGTITHKEAPVIK
RVYDQMCDPKWVMAFGACATSGGVYRNYTVLQGVDRIIPVDIYIPGCPPRPEMVIDAIMKLQDKIAGERHPIFPYEKQNP
VPV
>Mature_162_residues
AMMEYLTTRKDELIGWVRKFSIFPYPFVTACCGMEFMAVASTLYDTDRFGAALPRFTPRQSDLLMVVGTITHKEAPVIKR
VYDQMCDPKWVMAFGACATSGGVYRNYTVLQGVDRIIPVDIYIPGCPPRPEMVIDAIMKLQDKIAGERHPIFPYEKQNPV
PV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=154, Percent_Identity=47.4025974025974, Blast_Score=159, Evalue=1e-39,
Organism=Escherichia coli, GI1788624, Length=141, Percent_Identity=53.1914893617021, Blast_Score=170, Evalue=5e-44,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=33.8129496402878, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI1788834, Length=118, Percent_Identity=35.5932203389831, Blast_Score=80, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=154, Percent_Identity=45.4545454545455, Blast_Score=152, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=47.5862068965517, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=47.5862068965517, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651058, Length=154, Percent_Identity=46.1038961038961, Blast_Score=155, Evalue=1e-38,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_PELPD (A1ALP0)

Other databases:

- EMBL:   CP000482
- EMBL:   CP000482
- EMBL:   CP000482
- RefSeq:   YP_900318.1
- RefSeq:   YP_901297.1
- RefSeq:   YP_902843.1
- ProteinModelPortal:   A1ALP0
- SMR:   A1ALP0
- STRING:   A1ALP0
- GeneID:   4571841
- GeneID:   4571934
- GeneID:   4573259
- GenomeReviews:   CP000482_GR
- GenomeReviews:   CP000482_GR
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0629
- KEGG:   ppd:Ppro_1624
- KEGG:   ppd:Ppro_3192
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   LTKFDQF
- PhylomeDB:   A1ALP0
- ProtClustDB:   CLSK2470814
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 18476; Mature: 18345

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMMEYLTTRKDELIGWVRKFSIFPYPFVTACCGMEFMAVASTLYDTDRFGAALPRFTPR
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QSDLLMVVGTITHKEAPVIKRVYDQMCDPKWVMAFGACATSGGVYRNYTVLQGVDRIIPV
CCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEE
DIYIPGCPPRPEMVIDAIMKLQDKIAGERHPIFPYEKQNPVPV
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMMEYLTTRKDELIGWVRKFSIFPYPFVTACCGMEFMAVASTLYDTDRFGAALPRFTPR
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QSDLLMVVGTITHKEAPVIKRVYDQMCDPKWVMAFGACATSGGVYRNYTVLQGVDRIIPV
CCCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEE
DIYIPGCPPRPEMVIDAIMKLQDKIAGERHPIFPYEKQNPVPV
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA