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The map label for this gene is ackA [H]

Identifier: 118579047

GI number: 118579047

Start: 643525

End: 644790

Strand: Direct

Name: ackA [H]

Synonym: Ppro_0608

Alternate gene names: 118579047

Gene position: 643525-644790 (Clockwise)

Preceding gene: 118579046

Following gene: 118579048

Centisome position: 16.06

GC content: 60.82

Gene sequence:

>1266_bases
ATGATCATCATGGCATTGAACTGCGGCAGTTCGTCGGTCAAGTACCAGCTCTATGACTGGAGTCGTAAAGAAGTGGTGGC
CAAGGGCATTGTCGAGCGGGTCACCATCGGTGATTCGTTTATCGTGCATGAGGTCCCTGGTAGGGACGCTTTTAAGGAGG
AGCAGAACTGTCCCACCCACCAGGCTGCCATCGATCTGATCATCAGGACGGTCACCGACCCGAAGCGGGGCGTTATCAAG
GATGTCTCCGAAATCGCCGCCGTGGGTCACCGCATTGTGCATGGTGGAGAGAAGTTTACCCGTTCGGTGGTCATCGACGA
TCAGGTTCTGGAGGCGGTCAAGGAGTGCCAGCACCTGGCGCCGCTGCACAACCCGCCCAACATCGAGGGGGTCCTGGCTG
CGCGGGCCCTCCTGCCCACTGTTCCGCAGATTGCTGTCTTCGACACCGCCTTTCACCAGACCATGCCGGAATATGCCTAC
ATCTACCCGGTTCCCTACGAGTGGTACCAGAAGCACCAGGTGCGGCGCTACGGCTTCCACGGCACCTCCCACCTGTACGT
CGCCAAGCGCGCGGCGGCGCTGCTGGGCAAGAAACCGGCCGACACCAACCTGGTCACCCTGCACATCGGCAACGGCGTCT
CCCACTGCGCCATCAAGAACGGCATCTCCATCGACACCTCCATGGGGCTCACCCCGCTGGAAGGGGCCATCATGGGCACT
CGCTGCGGCGACATCGACCCGGCCATCCCGCTGTTCATGATGCATAAGGAGGGTTTGAGCGTCAAGGATATCGACACCAT
CCTGAACAAGAAGAGCGGCATCTTGGGCATAACCGGCCGTTTCTCCGACCGCCGCGACGTGGCCCGGCATGCCGAGGCCG
GCGACGAGCGCTGCAAGCTGGCCATGAGGATCGAGGTATTCCGCATCAAAAAGTACATCGGCGCCTACATGGCCATCGTG
GGCAAACTGGACGCCGTCGTCTTCACCGCCGGAGTGGGCGAGATGAGCGTCAAGGTCCGCGAGATGGTTCTGGAAGGGAT
GGAACATCTGGGGCTGATCCTGGACAGGGAGCGTAACAATAGCGCCATGACCCGTAAGAAGGAGACACTGATCACCACCG
ACGACTCCCCGGTCAAGGCCTTCGTCATCCCCACCGACGAGGAGCTGGTCCTGACCGAGGATGTGGCTGCCATCCTGAAC
GGCACCTACGCCGACCACATGAACTTCGAGTACGCCTTCTCCCGGCCGGATTTCGTGCGCACATAG

Upstream 100 bases:

>100_bases
GATGTGATCACACGGACGTCAGACGTGATTTCTCTCCGTAACCAGGATTAGCGATGTTTTGTTGTTTACCAGAGCGCTTG
CCGTGCAAGGAGGTCTGGCA

Downstream 100 bases:

>100_bases
CCGGGGCGGAATGTTTCGGAAACGACAAAGCCGGCTAAATGCCGGCTTTGTCGTTTCCCGCGTTGCGTGCTACAATCACC
CCACCATGTCAAACCCCAAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MIIMALNCGSSSVKYQLYDWSRKEVVAKGIVERVTIGDSFIVHEVPGRDAFKEEQNCPTHQAAIDLIIRTVTDPKRGVIK
DVSEIAAVGHRIVHGGEKFTRSVVIDDQVLEAVKECQHLAPLHNPPNIEGVLAARALLPTVPQIAVFDTAFHQTMPEYAY
IYPVPYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGT
RCGDIDPAIPLFMMHKEGLSVKDIDTILNKKSGILGITGRFSDRRDVARHAEAGDERCKLAMRIEVFRIKKYIGAYMAIV
GKLDAVVFTAGVGEMSVKVREMVLEGMEHLGLILDRERNNSAMTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILN
GTYADHMNFEYAFSRPDFVRT

Sequences:

>Translated_421_residues
MIIMALNCGSSSVKYQLYDWSRKEVVAKGIVERVTIGDSFIVHEVPGRDAFKEEQNCPTHQAAIDLIIRTVTDPKRGVIK
DVSEIAAVGHRIVHGGEKFTRSVVIDDQVLEAVKECQHLAPLHNPPNIEGVLAARALLPTVPQIAVFDTAFHQTMPEYAY
IYPVPYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGT
RCGDIDPAIPLFMMHKEGLSVKDIDTILNKKSGILGITGRFSDRRDVARHAEAGDERCKLAMRIEVFRIKKYIGAYMAIV
GKLDAVVFTAGVGEMSVKVREMVLEGMEHLGLILDRERNNSAMTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILN
GTYADHMNFEYAFSRPDFVRT
>Mature_421_residues
MIIMALNCGSSSVKYQLYDWSRKEVVAKGIVERVTIGDSFIVHEVPGRDAFKEEQNCPTHQAAIDLIIRTVTDPKRGVIK
DVSEIAAVGHRIVHGGEKFTRSVVIDDQVLEAVKECQHLAPLHNPPNIEGVLAARALLPTVPQIAVFDTAFHQTMPEYAY
IYPVPYEWYQKHQVRRYGFHGTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGT
RCGDIDPAIPLFMMHKEGLSVKDIDTILNKKSGILGITGRFSDRRDVARHAEAGDERCKLAMRIEVFRIKKYIGAYMAIV
GKLDAVVFTAGVGEMSVKVREMVLEGMEHLGLILDRERNNSAMTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILN
GTYADHMNFEYAFSRPDFVRT

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=44.2211055276382, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=40.7124681933842, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 46683; Mature: 46683

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIMALNCGSSSVKYQLYDWSRKEVVAKGIVERVTIGDSFIVHEVPGRDAFKEEQNCPTH
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHCCCHH
QAAIDLIIRTVTDPKRGVIKDVSEIAAVGHRIVHGGEKFTRSVVIDDQVLEAVKECQHLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHC
PLHNPPNIEGVLAARALLPTVPQIAVFDTAFHQTMPEYAYIYPVPYEWYQKHQVRRYGFH
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCC
GTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGT
CCCEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCCCCCCEEEC
RCGDIDPAIPLFMMHKEGLSVKDIDTILNKKSGILGITGRFSDRRDVARHAEAGDERCKL
CCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHCEE
AMRIEVFRIKKYIGAYMAIVGKLDAVVFTAGVGEMSVKVREMVLEGMEHLGLILDRERNN
EHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCC
SAMTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYADHMNFEYAFSRPDFVR
CHHHCCCCEEEECCCCCEEEEEECCCCCEEEHHHHHHHHCCCCHHCCCEEEEECCCCCCC
T
C
>Mature Secondary Structure
MIIMALNCGSSSVKYQLYDWSRKEVVAKGIVERVTIGDSFIVHEVPGRDAFKEEQNCPTH
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHEECCCCEEEEECCCCCCHHHHHCCCHH
QAAIDLIIRTVTDPKRGVIKDVSEIAAVGHRIVHGGEKFTRSVVIDDQVLEAVKECQHLA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHC
PLHNPPNIEGVLAARALLPTVPQIAVFDTAFHQTMPEYAYIYPVPYEWYQKHQVRRYGFH
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCC
GTSHLYVAKRAAALLGKKPADTNLVTLHIGNGVSHCAIKNGISIDTSMGLTPLEGAIMGT
CCCEEHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHCCCCCEEECCCCCCCCCCCEEEC
RCGDIDPAIPLFMMHKEGLSVKDIDTILNKKSGILGITGRFSDRRDVARHAEAGDERCKL
CCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCHHHCEE
AMRIEVFRIKKYIGAYMAIVGKLDAVVFTAGVGEMSVKVREMVLEGMEHLGLILDRERNN
EHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCC
SAMTRKKETLITTDDSPVKAFVIPTDEELVLTEDVAAILNGTYADHMNFEYAFSRPDFVR
CHHHCCCCEEEECCCCCEEEEEECCCCCEEEHHHHHHHHCCCCHHCCCEEEEECCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA