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The map label for this gene is hyfB [H]

Identifier: 118578518

GI number: 118578518

Start: 80691

End: 82694

Strand: Direct

Name: hyfB [H]

Synonym: Ppro_0070

Alternate gene names: 118578518

Gene position: 80691-82694 (Clockwise)

Preceding gene: 118578517

Following gene: 118578526

Centisome position: 2.01

GC content: 65.92

Gene sequence:

>2004_bases
ATGGAGCAGGAGCTGGCTTCACCCTGTCTGCTGATAGTGGCCGCCGCGCTTGTGGCGGCCCTGTCCGGTGTTCCGCTGTT
GATTTCTCCGCCATCGGGCGGGGTTCGTGGTCAACGGTCGGCAACAGTCCTCATGCTGATCGCCGCCTGCACCGGCCTGA
CCGGGGCGATACTAGTGCTTCTCTCCGCTGCATCAGAAACCTATCAGCTCTTCTGGTCACTCCCCTTCGGCCGGGCCGAG
ATCGGCATCGACCCGCTCTCGGCCTGGTTTCTGCTCCCCATCTTCCTGATTCCCGGCTGCGCCTCCCTGTACGCCCGCGG
CTACTGGCCCGCCGAATCCCATCCCGGCAACGTGCGGCGCATGACCTTCTTCTTCGGCCTGATGACGGCCGCCATGGCCG
GCGTGGTCCTGGCCCGCGACGCCGTCACCTTCCTGTTCGCCTGGGAGATCATGGCCATCTCGGCCTACTTCCTGGTCTCC
ACCGAGGATGAAAAGCGCGAGGTGAGCGAGGCCGGCACCCTGTACCTGATCACCACCCACATCGCCACCCTGGGCCTGTT
CGCCCTCTTTTCCCTGCTGAACCTGCTGGATGGTTCCTGGCTCTTCCCGGCTACCGGCGCCCTGGATGCCTCGTCTCCCC
TGGCAACGGCCGTTTTCCTGGCCGCCCTGCTCGGTTTCGGCATGAAGGCCGGCATCATGCCGCTGCACATCTGGCTCCCG
TCGGCCCACGCCAATGCGCCCAGCCACGTTTCCGCAGCCCTGTCGGGGGTCATCCTCAAGGTGGGTATCTATGGCCTGGT
GCGCACCCTCTCCTTCTTCCACGGTCTTCCGCTTTGGTGGGGATACATCGTGCTGCTGCTGGGGATCGTTTCCGGCGTGG
CAGGAGTGGCCTTCGCCATCGGTCAGCACGACCTGAAGCGGCTCCTGGCCTATCACAGCATCGAAAACATCGGCATTATC
ACCATGGGGCTGGGGGTGGCGCTGATCGGGCGGACCATCGACTCGCCGGCCATGGTGCTCCTGGGCATGGCTGGAGCGCT
GCTGCATGTCCTCAACCATGCCGTGTTCAAGGCGCTGCTCTTCATGGGGGCCGGCTCCATCATCCACGCCAGTGGTACCC
GCGAAATCGACCTGATGGGGGGGCTTGGCCGCAAGCTGCCCGCTACGGCCCTGCTGTTCGGGGTGGGCGCGGTGGCCATC
TGCGGTCTGCCGCCGCTGAACGGCTTTGTCAGTGAACTGCTGATCTATCTGGGCTGTTTCAGGGGGGTGCAGGGGGACGG
CAGCAGTGCTGCCATGACCGCCCTGGCCGCGCCGGCTCTGGCGCTGATCGGCGGGCTGGCCGTGGCCTGCTTCGTCAAGG
TCTACGGCATCGTTTTCCTGGGAATGGCACGCACGCACAAGCATGGACAGGGACATGAGGCCGGCTGGCAGATGCTGCTC
CCCATGGGGCTGCTGGCGCTGGTCTGCCTGGCTGTCGGCCTGTTCCCCGCTCTCCTGGCCGGCCTGCTGGAGAATGCCTC
CCGGGCCTTCCTGCCGACCGGGATGCCTGCCCTTCAGCCGCTGGCCCTTCTGTCGCCGTTTGGGATGGTGACGCTCATGG
CCCTTGGCCTGCTGACCCTGATGGCCGCTCTGGCCCTCTGGTACCGTTGGCGGTTGGCCCGCGCGCCCAGGGGAGAAACC
ATCACCTGGAGCTGCGGCTACCAGCGCCCGGCGCCCCGCATACAGTACAGCGCCTCCTCATTTGCCCAGATGCTGACCGG
ACTGTTCTCCTTCGTGCTGCGACAGGAGAGCCAGCTTCCCGCCATTGCCGGGCCCTTTCCCCGGGCCAGCCGCTTCCACA
GCCACGTTCCCGAGGCGGTGCTGGAGCTGGTTTACATTCCGGCCCTGAAGCGCCTGCACCGGCGTTTCAGCGGGGTGCGC
CGGTTGCAGGGGGGGCTGCTGCAGCAGTACATGCTCTACATCCTGCTGACCCTGGTGGCGCTGTTTCTCTGCGACTCCAT
CTGA

Upstream 100 bases:

>100_bases
ACCAGCAGTTCAAGGATCTGTTGCACCGTACCGGCAGCGCGGCCGAGATCATGGCCGCCATCCGGGCGATGGAGGAAACC
ATCATCAGGAGGTAAGTGTC

Downstream 100 bases:

>100_bases
CCTCTTCTCCCGATGGCCTTTGGGATCTCGACAGCCAGATCAGGTGGCCATGATAATGCCCGCCTCCGCTCATGTGATGC
GCTTGACGTTCATGATCCTG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MEQELASPCLLIVAAALVAALSGVPLLISPPSGGVRGQRSATVLMLIAACTGLTGAILVLLSAASETYQLFWSLPFGRAE
IGIDPLSAWFLLPIFLIPGCASLYARGYWPAESHPGNVRRMTFFFGLMTAAMAGVVLARDAVTFLFAWEIMAISAYFLVS
TEDEKREVSEAGTLYLITTHIATLGLFALFSLLNLLDGSWLFPATGALDASSPLATAVFLAALLGFGMKAGIMPLHIWLP
SAHANAPSHVSAALSGVILKVGIYGLVRTLSFFHGLPLWWGYIVLLLGIVSGVAGVAFAIGQHDLKRLLAYHSIENIGII
TMGLGVALIGRTIDSPAMVLLGMAGALLHVLNHAVFKALLFMGAGSIIHASGTREIDLMGGLGRKLPATALLFGVGAVAI
CGLPPLNGFVSELLIYLGCFRGVQGDGSSAAMTALAAPALALIGGLAVACFVKVYGIVFLGMARTHKHGQGHEAGWQMLL
PMGLLALVCLAVGLFPALLAGLLENASRAFLPTGMPALQPLALLSPFGMVTLMALGLLTLMAALALWYRWRLARAPRGET
ITWSCGYQRPAPRIQYSASSFAQMLTGLFSFVLRQESQLPAIAGPFPRASRFHSHVPEAVLELVYIPALKRLHRRFSGVR
RLQGGLLQQYMLYILLTLVALFLCDSI

Sequences:

>Translated_667_residues
MEQELASPCLLIVAAALVAALSGVPLLISPPSGGVRGQRSATVLMLIAACTGLTGAILVLLSAASETYQLFWSLPFGRAE
IGIDPLSAWFLLPIFLIPGCASLYARGYWPAESHPGNVRRMTFFFGLMTAAMAGVVLARDAVTFLFAWEIMAISAYFLVS
TEDEKREVSEAGTLYLITTHIATLGLFALFSLLNLLDGSWLFPATGALDASSPLATAVFLAALLGFGMKAGIMPLHIWLP
SAHANAPSHVSAALSGVILKVGIYGLVRTLSFFHGLPLWWGYIVLLLGIVSGVAGVAFAIGQHDLKRLLAYHSIENIGII
TMGLGVALIGRTIDSPAMVLLGMAGALLHVLNHAVFKALLFMGAGSIIHASGTREIDLMGGLGRKLPATALLFGVGAVAI
CGLPPLNGFVSELLIYLGCFRGVQGDGSSAAMTALAAPALALIGGLAVACFVKVYGIVFLGMARTHKHGQGHEAGWQMLL
PMGLLALVCLAVGLFPALLAGLLENASRAFLPTGMPALQPLALLSPFGMVTLMALGLLTLMAALALWYRWRLARAPRGET
ITWSCGYQRPAPRIQYSASSFAQMLTGLFSFVLRQESQLPAIAGPFPRASRFHSHVPEAVLELVYIPALKRLHRRFSGVR
RLQGGLLQQYMLYILLTLVALFLCDSI
>Mature_667_residues
MEQELASPCLLIVAAALVAALSGVPLLISPPSGGVRGQRSATVLMLIAACTGLTGAILVLLSAASETYQLFWSLPFGRAE
IGIDPLSAWFLLPIFLIPGCASLYARGYWPAESHPGNVRRMTFFFGLMTAAMAGVVLARDAVTFLFAWEIMAISAYFLVS
TEDEKREVSEAGTLYLITTHIATLGLFALFSLLNLLDGSWLFPATGALDASSPLATAVFLAALLGFGMKAGIMPLHIWLP
SAHANAPSHVSAALSGVILKVGIYGLVRTLSFFHGLPLWWGYIVLLLGIVSGVAGVAFAIGQHDLKRLLAYHSIENIGII
TMGLGVALIGRTIDSPAMVLLGMAGALLHVLNHAVFKALLFMGAGSIIHASGTREIDLMGGLGRKLPATALLFGVGAVAI
CGLPPLNGFVSELLIYLGCFRGVQGDGSSAAMTALAAPALALIGGLAVACFVKVYGIVFLGMARTHKHGQGHEAGWQMLL
PMGLLALVCLAVGLFPALLAGLLENASRAFLPTGMPALQPLALLSPFGMVTLMALGLLTLMAALALWYRWRLARAPRGET
ITWSCGYQRPAPRIQYSASSFAQMLTGLFSFVLRQESQLPAIAGPFPRASRFHSHVPEAVLELVYIPALKRLHRRFSGVR
RLQGGLLQQYMLYILLTLVALFLCDSI

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=306, Percent_Identity=28.4313725490196, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI251831116, Length=286, Percent_Identity=27.6223776223776, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788827, Length=618, Percent_Identity=35.2750809061489, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI2367154, Length=529, Percent_Identity=34.0264650283554, Blast_Score=222, Evalue=7e-59,
Organism=Escherichia coli, GI1788831, Length=353, Percent_Identity=33.7110481586402, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1788613, Length=304, Percent_Identity=32.5657894736842, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788829, Length=347, Percent_Identity=29.6829971181556, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788614, Length=391, Percent_Identity=31.7135549872123, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70780; Mature: 70780

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQELASPCLLIVAAALVAALSGVPLLISPPSGGVRGQRSATVLMLIAACTGLTGAILVL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHH
LSAASETYQLFWSLPFGRAEIGIDPLSAWFLLPIFLIPGCASLYARGYWPAESHPGNVRR
HHHCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHH
MTFFFGLMTAAMAGVVLARDAVTFLFAWEIMAISAYFLVSTEDEKREVSEAGTLYLITTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCEEEHHHH
IATLGLFALFSLLNLLDGSWLFPATGALDASSPLATAVFLAALLGFGMKAGIMPLHIWLP
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEEEEC
SAHANAPSHVSAALSGVILKVGIYGLVRTLSFFHGLPLWWGYIVLLLGIVSGVAGVAFAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GQHDLKRLLAYHSIENIGIITMGLGVALIGRTIDSPAMVLLGMAGALLHVLNHAVFKALL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FMGAGSIIHASGTREIDLMGGLGRKLPATALLFGVGAVAICGLPPLNGFVSELLIYLGCF
HHCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGVQGDGSSAAMTALAAPALALIGGLAVACFVKVYGIVFLGMARTHKHGQGHEAGWQMLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
PMGLLALVCLAVGLFPALLAGLLENASRAFLPTGMPALQPLALLSPFGMVTLMALGLLTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
MAALALWYRWRLARAPRGETITWSCGYQRPAPRIQYSASSFAQMLTGLFSFVLRQESQLP
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
AIAGPFPRASRFHSHVPEAVLELVYIPALKRLHRRFSGVRRLQGGLLQQYMLYILLTLVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLCDSI
HHHHCCC
>Mature Secondary Structure
MEQELASPCLLIVAAALVAALSGVPLLISPPSGGVRGQRSATVLMLIAACTGLTGAILVL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHH
LSAASETYQLFWSLPFGRAEIGIDPLSAWFLLPIFLIPGCASLYARGYWPAESHPGNVRR
HHHCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHH
MTFFFGLMTAAMAGVVLARDAVTFLFAWEIMAISAYFLVSTEDEKREVSEAGTLYLITTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCEEEHHHH
IATLGLFALFSLLNLLDGSWLFPATGALDASSPLATAVFLAALLGFGMKAGIMPLHIWLP
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEEEEC
SAHANAPSHVSAALSGVILKVGIYGLVRTLSFFHGLPLWWGYIVLLLGIVSGVAGVAFAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GQHDLKRLLAYHSIENIGIITMGLGVALIGRTIDSPAMVLLGMAGALLHVLNHAVFKALL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FMGAGSIIHASGTREIDLMGGLGRKLPATALLFGVGAVAICGLPPLNGFVSELLIYLGCF
HHCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGVQGDGSSAAMTALAAPALALIGGLAVACFVKVYGIVFLGMARTHKHGQGHEAGWQMLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
PMGLLALVCLAVGLFPALLAGLLENASRAFLPTGMPALQPLALLSPFGMVTLMALGLLTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
MAALALWYRWRLARAPRGETITWSCGYQRPAPRIQYSASSFAQMLTGLFSFVLRQESQLP
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCCC
AIAGPFPRASRFHSHVPEAVLELVYIPALKRLHRRFSGVRRLQGGLLQQYMLYILLTLVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLCDSI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]