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The map label for this gene is ptsN [H]

Identifier: 118578517

GI number: 118578517

Start: 80008

End: 80685

Strand: Direct

Name: ptsN [H]

Synonym: Ppro_0069

Alternate gene names: 118578517

Gene position: 80008-80685 (Clockwise)

Preceding gene: 118578516

Following gene: 118578518

Centisome position: 2.0

GC content: 61.65

Gene sequence:

>678_bases
ATGTTTTTACGAGTAGAGGATATAGCACAGGCTGTGGGTGTCAGCGAACGGACGGTCCTGGGCTGGATCAAAAACCAGGG
GCTTCCGGCGCATCTTCAGAACGACCGCTACCGCATCAACCGGGTCGACCTGCTGGAATGGGCCACCAGTCACGACATCA
CCATCCCGCCGGATGTCTTTGACAGCGGCTCGGAAACCCCGCGGCTCTCGACGCTCTCCGAGGCTCTGGAGGCGGGGGGG
ATCCATTACGACCTGGCTGGCGACACCCCGGAATCGGCGCTGCGCCAGGTGATCGGCCGCATGAAGCTCCCCCCCAGCCT
GGATCCGGAATTCCTGCTGCAGACCTTTCTGGCGCGCGAGGCCCTGGGCTCCACCGCCCTGGGGAACGGCATCGCCATCC
CGCATGTGCGCAACCCCATCGTGGGGCAGGTTCAGGAATCTGCCATCAGCCTCTGCTTCCTGAAGAACCCCATCGACTTC
GATGCCATCGACGGCCAGCCGGTCAGCATTCTCTTCACCCTGATTACGCCCAATGTGAAAACCCATCTGCATCTGCTGGC
AAAGCTGGCGTTCATGCTCCATGACCAGCAGTTCAAGGATCTGTTGCACCGTACCGGCAGCGCGGCCGAGATCATGGCCG
CCATCCGGGCGATGGAGGAAACCATCATCAGGAGGTAA

Upstream 100 bases:

>100_bases
TGACGTTTACCGGGTGATGGCATATTGTTTTCAGTCAGCCGCCCTGGGAGCGCCAAGTCCTTGGGTGGCGCATCGCACTA
CCAGGATGGAGAATTTCGAA

Downstream 100 bases:

>100_bases
GTGTCATGGAGCAGGAGCTGGCTTCACCCTGTCTGCTGATAGTGGCCGCCGCGCTTGTGGCGGCCCTGTCCGGTGTTCCG
CTGTTGATTTCTCCGCCATC

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MFLRVEDIAQAVGVSERTVLGWIKNQGLPAHLQNDRYRINRVDLLEWATSHDITIPPDVFDSGSETPRLSTLSEALEAGG
IHYDLAGDTPESALRQVIGRMKLPPSLDPEFLLQTFLAREALGSTALGNGIAIPHVRNPIVGQVQESAISLCFLKNPIDF
DAIDGQPVSILFTLITPNVKTHLHLLAKLAFMLHDQQFKDLLHRTGSAAEIMAAIRAMEETIIRR

Sequences:

>Translated_225_residues
MFLRVEDIAQAVGVSERTVLGWIKNQGLPAHLQNDRYRINRVDLLEWATSHDITIPPDVFDSGSETPRLSTLSEALEAGG
IHYDLAGDTPESALRQVIGRMKLPPSLDPEFLLQTFLAREALGSTALGNGIAIPHVRNPIVGQVQESAISLCFLKNPIDF
DAIDGQPVSILFTLITPNVKTHLHLLAKLAFMLHDQQFKDLLHRTGSAAEIMAAIRAMEETIIRR
>Mature_225_residues
MFLRVEDIAQAVGVSERTVLGWIKNQGLPAHLQNDRYRINRVDLLEWATSHDITIPPDVFDSGSETPRLSTLSEALEAGG
IHYDLAGDTPESALRQVIGRMKLPPSLDPEFLLQTFLAREALGSTALGNGIAIPHVRNPIVGQVQESAISLCFLKNPIDF
DAIDGQPVSILFTLITPNVKTHLHLLAKLAFMLHDQQFKDLLHRTGSAAEIMAAIRAMEETIIRR

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=122, Percent_Identity=40.1639344262295, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 24845; Mature: 24845

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRVEDIAQAVGVSERTVLGWIKNQGLPAHLQNDRYRINRVDLLEWATSHDITIPPDVF
CEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCCC
DSGSETPRLSTLSEALEAGGIHYDLAGDTPESALRQVIGRMKLPPSLDPEFLLQTFLARE
CCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ALGSTALGNGIAIPHVRNPIVGQVQESAISLCFLKNPIDFDAIDGQPVSILFTLITPNVK
HHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHH
THLHLLAKLAFMLHDQQFKDLLHRTGSAAEIMAAIRAMEETIIRR
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLRVEDIAQAVGVSERTVLGWIKNQGLPAHLQNDRYRINRVDLLEWATSHDITIPPDVF
CEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCCC
DSGSETPRLSTLSEALEAGGIHYDLAGDTPESALRQVIGRMKLPPSLDPEFLLQTFLARE
CCCCCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ALGSTALGNGIAIPHVRNPIVGQVQESAISLCFLKNPIDFDAIDGQPVSILFTLITPNVK
HHCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHH
THLHLLAKLAFMLHDQQFKDLLHRTGSAAEIMAAIRAMEETIIRR
HHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]