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The map label for this gene is ispF

Identifier: 118578461

GI number: 118578461

Start: 15002

End: 15478

Strand: Direct

Name: ispF

Synonym: Ppro_0012

Alternate gene names: 118578461

Gene position: 15002-15478 (Clockwise)

Preceding gene: 118578460

Following gene: 118578463

Centisome position: 0.37

GC content: 63.31

Gene sequence:

>477_bases
ATGCGCATCGGACACGGCTACGACGTCCACAAACTGGTTGAGGGGAGAAAGCTGATCATGGGGGGGGTGGATATCCCCTG
GGAGAAGGGACTGTTGGGGCATTCCGACGCCGACGTGTTGCTGCACGCCATTGCCGACGCCATCCTGGGCGCCCTGGGCG
AGGGGGATATCGGCAAGCATTTCCCCGACACCGATCCGGCATACAAGGGGGCCGACAGCCTGAAGCTTCTGGAGCATGTG
GCTAATCTGGCGATCGCGAAGGGGTACTGCCTGGGCAACCTGGACGCCACCATCATCGCCCAGCGTCCCAAGATGGCGCC
TCACATCCCTGCCATGCGGGAGAACATCGCCCGGGTGCTGAGGAGCACGGTGGATCGGGTCAATGTCAAGGCCACCACCG
AGGAGGGCTTGAGCTTCACCGGCAGGGGGGAAGGGATCTCGGCCCATGCCGTGGTGCTGATGATCGGTAAGGGGTGA

Upstream 100 bases:

>100_bases
CTCCTGGCGGATGAAGACGCGGGGGGCGGTGATGTGGGGACAGTTGGCTAACCCCGAATGAAACCAAAAATTACTAATAA
ACTCAAGGAGATACCATAAC

Downstream 100 bases:

>100_bases
TTCAAGTTGGAAAAGGCGGATACTCACGCAAAGGCGCAACGTTCGCGAAGACAACCGAAAACGTATCTAAAACAAACGAC
TCACTCCATGCGGGGAGTTG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRIGHGYDVHKLVEGRKLIMGGVDIPWEKGLLGHSDADVLLHAIADAILGALGEGDIGKHFPDTDPAYKGADSLKLLEHV
ANLAIAKGYCLGNLDATIIAQRPKMAPHIPAMRENIARVLRSTVDRVNVKATTEEGLSFTGRGEGISAHAVVLMIGKG

Sequences:

>Translated_158_residues
MRIGHGYDVHKLVEGRKLIMGGVDIPWEKGLLGHSDADVLLHAIADAILGALGEGDIGKHFPDTDPAYKGADSLKLLEHV
ANLAIAKGYCLGNLDATIIAQRPKMAPHIPAMRENIARVLRSTVDRVNVKATTEEGLSFTGRGEGISAHAVVLMIGKG
>Mature_158_residues
MRIGHGYDVHKLVEGRKLIMGGVDIPWEKGLLGHSDADVLLHAIADAILGALGEGDIGKHFPDTDPAYKGADSLKLLEHV
ANLAIAKGYCLGNLDATIIAQRPKMAPHIPAMRENIARVLRSTVDRVNVKATTEEGLSFTGRGEGISAHAVVLMIGKG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=63.8709677419355, Blast_Score=182, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PELPD (A1AJY3)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_899711.1
- ProteinModelPortal:   A1AJY3
- SMR:   A1AJY3
- STRING:   A1AJY3
- GeneID:   4573884
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0012
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A1AJY3
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16790; Mature: 16790

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGYDVHKLVEGRKLIMGGVDIPWEKGLLGHSDADVLLHAIADAILGALGEGDIGKH
CCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
FPDTDPAYKGADSLKLLEHVANLAIAKGYCLGNLDATIIAQRPKMAPHIPAMRENIARVL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
RSTVDRVNVKATTEEGLSFTGRGEGISAHAVVLMIGKG
HHHHHHCCCEEECCCCCEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRIGHGYDVHKLVEGRKLIMGGVDIPWEKGLLGHSDADVLLHAIADAILGALGEGDIGKH
CCCCCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
FPDTDPAYKGADSLKLLEHVANLAIAKGYCLGNLDATIIAQRPKMAPHIPAMRENIARVL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
RSTVDRVNVKATTEEGLSFTGRGEGISAHAVVLMIGKG
HHHHHHCCCEEECCCCCEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA