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The map label for this gene is lplA [H]

Identifier: 118479396

GI number: 118479396

Start: 4026060

End: 4026908

Strand: Reverse

Name: lplA [H]

Synonym: BALH_3813

Alternate gene names: 118479396

Gene position: 4026908-4026060 (Counterclockwise)

Preceding gene: 118479401

Following gene: 118479393

Centisome position: 76.6

GC content: 35.1

Gene sequence:

>849_bases
ATGAGGTGCAGGATGGGAAAAGAAAAATGGTGTTATATTAACTCTGGTCAATGTTCACCGGCATTTAATATGGCGTTAGA
TGAATGTTTATTAAATTGGCAAAGTGAAAAGAAAATGCCACCAACTATTCGTTTTTACGAATGGGAAGTACCAACATTAA
CAGTCGGATATTTCCAGCGTGTTGAAAAAGATATTAATATGGATGTAGTTAACGAAAAGAAATATGGATTCGTTCGTCGT
CAAACAGGCGGCAGGGGTGTATTACATGATAAAGAATTAACGTATAGTGTTATTGTGTCTGAAGATCATCCGAATATGCC
AAAAACAGTTACAGAAGCGTATCGTGTTATTTCGCAAGGTTTACTAGATGGTTTTAAAGCATTAGGGTTAGAAGCGTATT
ATGCAGTTCCAAAAACAGAAGCAGATCGTGAAAATTTAAAAAATCCACGCTCAGGCGTATGTTTTGATGCACCGTCTTGG
TATGAAATTGTAGTAGAAGGAAGAAAAATCGCAGGTAGTGCGCAAACACGTCAAAAAGGTGTTATTTTGCAGCATGGATC
GATTCCGTTAGAAATAGATTTAGATGAGTTATATGATCTGTTTTTATTCCCGAATGAGCGTGTAAAAGAACGTATGAAGA
GTATGTTCTCTTCTAAAGCGGTAGCAATTAATGAATTAACAGACCGTACGTTTACAATCGAACAGTTAATTAAAGCGTTT
GAGGTTGGGTTTGAAAAAGGATTAGATGTAGAGCTTGTTCCATATGAATTAACAGAAGAACAATTACATGAAGTGCAAAC
ATTAGCAAAAGAAAAGTATGAAAGTAAAGAATGGAATTATAAAAAATAA

Upstream 100 bases:

>100_bases
TTATGCAACAGATTCATTTGTATATAAGAAGAAATTTCGTATTCTTTCACAAATTTTGTCGAATTTGGTAGACTAGAGAA
TGTGAAAAATAATTGGTTAT

Downstream 100 bases:

>100_bases
GAGGTGGCAATTGCCGCCTCTTATTTTTTTATAGTAAATTAAGTACTTTTTCTACTTTGATTCCTTTATCTTGTAAAGAA
CGAAGGCGATTGACAACGTC

Product: lipoate-protein ligase A

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRCRMGKEKWCYINSGQCSPAFNMALDECLLNWQSEKKMPPTIRFYEWEVPTLTVGYFQRVEKDINMDVVNEKKYGFVRR
QTGGRGVLHDKELTYSVIVSEDHPNMPKTVTEAYRVISQGLLDGFKALGLEAYYAVPKTEADRENLKNPRSGVCFDAPSW
YEIVVEGRKIAGSAQTRQKGVILQHGSIPLEIDLDELYDLFLFPNERVKERMKSMFSSKAVAINELTDRTFTIEQLIKAF
EVGFEKGLDVELVPYELTEEQLHEVQTLAKEKYESKEWNYKK

Sequences:

>Translated_282_residues
MRCRMGKEKWCYINSGQCSPAFNMALDECLLNWQSEKKMPPTIRFYEWEVPTLTVGYFQRVEKDINMDVVNEKKYGFVRR
QTGGRGVLHDKELTYSVIVSEDHPNMPKTVTEAYRVISQGLLDGFKALGLEAYYAVPKTEADRENLKNPRSGVCFDAPSW
YEIVVEGRKIAGSAQTRQKGVILQHGSIPLEIDLDELYDLFLFPNERVKERMKSMFSSKAVAINELTDRTFTIEQLIKAF
EVGFEKGLDVELVPYELTEEQLHEVQTLAKEKYESKEWNYKK
>Mature_282_residues
MRCRMGKEKWCYINSGQCSPAFNMALDECLLNWQSEKKMPPTIRFYEWEVPTLTVGYFQRVEKDINMDVVNEKKYGFVRR
QTGGRGVLHDKELTYSVIVSEDHPNMPKTVTEAYRVISQGLLDGFKALGLEAYYAVPKTEADRENLKNPRSGVCFDAPSW
YEIVVEGRKIAGSAQTRQKGVILQHGSIPLEIDLDELYDLFLFPNERVKERMKSMFSSKAVAINELTDRTFTIEQLIKAF
EVGFEKGLDVELVPYELTEEQLHEVQTLAKEKYESKEWNYKK

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 32666; Mature: 32666

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCRMGKEKWCYINSGQCSPAFNMALDECLLNWQSEKKMPPTIRFYEWEVPTLTVGYFQR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEHHHHHH
VEKDINMDVVNEKKYGFVRRQTGGRGVLHDKELTYSVIVSEDHPNMPKTVTEAYRVISQG
HHHCCCCEEECCCCCCEEEECCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LLDGFKALGLEAYYAVPKTEADRENLKNPRSGVCFDAPSWYEIVVEGRKIAGSAQTRQKG
HHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCEECCCCCEEEEEECCHHCCCCHHHHCC
VILQHGSIPLEIDLDELYDLFLFPNERVKERMKSMFSSKAVAINELTDRTFTIEQLIKAF
EEEECCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHCCCEEHHHHHHHHH
EVGFEKGLDVELVPYELTEEQLHEVQTLAKEKYESKEWNYKK
HHHHHCCCCEEEECHHCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRCRMGKEKWCYINSGQCSPAFNMALDECLLNWQSEKKMPPTIRFYEWEVPTLTVGYFQR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEHHHHHH
VEKDINMDVVNEKKYGFVRRQTGGRGVLHDKELTYSVIVSEDHPNMPKTVTEAYRVISQG
HHHCCCCEEECCCCCCEEEECCCCCCCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
LLDGFKALGLEAYYAVPKTEADRENLKNPRSGVCFDAPSWYEIVVEGRKIAGSAQTRQKG
HHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCEECCCCCEEEEEECCHHCCCCHHHHCC
VILQHGSIPLEIDLDELYDLFLFPNERVKERMKSMFSSKAVAINELTDRTFTIEQLIKAF
EEEECCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHCCCEEHHHHHHHHH
EVGFEKGLDVELVPYELTEEQLHEVQTLAKEKYESKEWNYKK
HHHHHCCCCEEEECHHCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]