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The map label for this gene is trmD

Identifier: 118479063

GI number: 118479063

Start: 3686135

End: 3686869

Strand: Reverse

Name: trmD

Synonym: BALH_3472

Alternate gene names: 118479063

Gene position: 3686869-3686135 (Counterclockwise)

Preceding gene: 118479064

Following gene: 118479062

Centisome position: 70.13

GC content: 39.05

Gene sequence:

>735_bases
ATGAAAATTGACATTTTAACATTGTTTCCAGATATGTTTACAGGTGTGTTTGGATCTTCAATTTTAAAGAAAGCACAAGA
AAAAGAAGCGGTAGAGCTTCGTGTTGTCAATTTCCGCGATTATACAACGAGCAAGCATAATAGCGTGGATGATTATCCGT
ATGGTGGTGGTGCTGGGATGGTATTAACTCCTCAGCCTATTTTTGATGCTGTAGAAGATTTAACGAAAGAGACAGAGCGT
AAGCCGAGAGTTGTCTTAATGTGTCCGCAGGGTGAAAGATTTACTCAGAAGAAAGCGGAAGAGCTTGCAGAGGAAGAACA
TTTAATCTTTGTATGTGGGCATTATGAAGGATATGACGAACGAATCCGTGAACATCTCGTAACAGATGAGATTTCTATTG
GTGACTACGTATTAACTGGTGGAGAGTTAGCCTCTATGGTTATTACCGATAGTGTTGTACGTCTCCTACCAGGAGTACTA
GGAAATCATGCTTCACAAGTCGAGGATTCGTTTAGTACTGGTTTATTAGAGCACCCTCACTATACACGTCCAGCTGATTT
TCGTGGTATGAAAGTACCGGATGTATTAATGTCAGGAAATCATAAAAATATTGATGAATGGCGACATAAAGAATCACTAC
GTCGTACATATACACGTAGACCGGATTTATTGGAAGAACGAGAATTGTCTAAGCAAGAAAAGAAATGGTTAGAACAGATT
AAAGAAGGCAAATAA

Upstream 100 bases:

>100_bases
AAGGTCAAGATTTGTTAATTCCTTATATTGATGATGTTGTGCTTCAAGTTAACATTGAGAATAAATTAGTAACCATTCAT
GTAATGGAAGGGCTACTATA

Downstream 100 bases:

>100_bases
GGGCTTGATATATTTTCTATTGCAACAGCCCTAGCAATATGCTATTATAACACTTGTGCTACTGAAATCAGTGGCCGTAT
TAACGATGTTCCGCTGTAAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAEEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLEERELSKQEKKWLEQI
KEGK

Sequences:

>Translated_244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAEEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLEERELSKQEKKWLEQI
KEGK
>Mature_244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAEEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLEERELSKQEKKWLEQI
KEGK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=45.643153526971, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BACAA (C3P5P2)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868078.1
- ProteinModelPortal:   C3P5P2
- SMR:   C3P5P2
- EnsemblBacteria:   EBBACT00000128939
- GeneID:   7852209
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4003
- GeneTree:   EBGT00050000001149
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27967; Mature: 27967

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTSKHNSVDDYPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEDLTKETERKPRVVLMCPQGERFTQKKAEELAEEEHLIFVCGHYEGYDE
EECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
RIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNHASQVEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLEERELSKQEKKWLEQI
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KEGK
HCCC
>Mature Secondary Structure
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVELRVVNFRDYTTSKHNSVDDYPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEDLTKETERKPRVVLMCPQGERFTQKKAEELAEEEHLIFVCGHYEGYDE
EECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHH
RIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNHASQVEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLEERELSKQEKKWLEQI
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KEGK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA