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The map label for this gene is opuE [H]

Identifier: 118478881

GI number: 118478881

Start: 3476142

End: 3477623

Strand: Reverse

Name: opuE [H]

Synonym: BALH_3280

Alternate gene names: 118478881

Gene position: 3477623-3476142 (Counterclockwise)

Preceding gene: 118478882

Following gene: 118478880

Centisome position: 66.15

GC content: 36.84

Gene sequence:

>1482_bases
TTGGTGAAGATTGAGATTATGGTTTCGCTTGCTATTTATATGGCAGGTATGTTGTATATCGGGTATTGGTCTTATAAGAA
GACATCCGATTTATCAGATTATATGTTAGGCGGAAGAGGACTCGGTCCAGCAGTTACAGCTTTATCAGCCGGTGCTTCTG
ACATGAGTGGTTGGATGCTTATGGGATTACCGGGTGCGATGTATGCGACAGGGTTGTCCAGTGTATGGATCGCGATAGGT
TTATTAATAGGCGCTTATGCAAACTATTTAATTCTCGCGCCGCGTTTACGAACATATACGGAAGTAGCAAATGATTCAAT
TACGATTCCAGATTTTTTAGAGAATCGGTTTAAAGATCGTACGAAAATACTTCGTTTTGTCTCCGCTATCGTCATTTTAG
TATTTTTCACATTTTATGCGTCAGCTGGTTTAGTTTCAGGTGGACGTTTGTTTGAAAATTCTTTTAACCTTGATTATAAA
ATTGGTTTATTTGTAACTGTCGGTGTCGTTGTTGCTTATACACTATTCGGTGGTTTTTTAGCAGTAAGTTGGACCGACTT
TGTGCAAGGTTGTATTATGTTTATTGCTCTTGTATTAGTTCCAATTGTAGCTTTTACAGATGTCGGTGGTGTAACAGAAA
CATTCAATACAATTAAGCAAGTTGATGCATCGCATTTAGATATGTTTAAAGGGACTACAATACTTGGTATTATTTCATTT
TTAGCATGGGGCCTTGGGTATTTTGGTCAACCACATATTATTGTCCGCTTTATGGCAATTACCTCTATTAAAGATTTAAA
AACTTCTCGTAGAATCGGTATCGGTTGGATGACGATTTCAATTATAGGTGCAATGCTTACTGGTCTAGTTGGTATTGCTT
ATTACGCTAAAAATAATGCGACATTACAAGATCCGGAAATGGTCTTTGTAACATTCTCAAATATTTTATTCCATCCGTAC
ATTACTGGATTTTTATTATCAGCTATTTTGGCTTCGATTATGAGTAGTATTTCTTCGCAATTACTAGTTATTTCAAGTGC
TGTAACGGAAGATTTCTATAAAACATTTTTCCGTCGTAAAGCAAGTGATAAAGAACTTGTATTTATCGGTAGGCTGTCAG
TATTAGTAGTAGCGATGATTGCAGTTGTTTTAGCGTATCATCCGAGTGATACAATTTTAACGCTTGTTGGATATGCTTGG
GCAGGATTTGGATCAGCATTCGGACCAGCAATTTTATTAAGTTTATATTGGAAGAGAACGAACAAATGGGGCGTTCTTGC
TGGGATGATTGTCGGTGCATTAGTTGTTATCACTTGGGTACAAATTCCAAGTTTAAAAGCGACTATGTATGAGATGGTAC
CTGGATTCTTCTGTAGCTTATTAGCTGTTATTATCGTAAGTTTAGTAACGAAAGAACCAGTTAAAGCAATACATCGTGAA
TTTAATGAGATGGAAGCAGTGCTGGAAGAAGAAACGAAATGA

Upstream 100 bases:

>100_bases
AAAAAATGTTTGTTAAGGTAGGGAACCATAAGCGAAAACAAGAAAATAGAAAAAGTCATTTTTCGAAATTTTGAATGGCT
TATGTAACTAAGGGAGAGAT

Downstream 100 bases:

>100_bases
TTTGAAAAGGGAGTAAACGGTGAATAGCGTTTACTCCCTTTTCATTTTAAATGAAATAAGTAACTTGTTGACAAAGAAAA
GTAAGTAACTTATAATAAGT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MVKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIG
LLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYK
IGLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISF
LAWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPY
ITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAW
AGFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKATMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHRE
FNEMEAVLEEETK

Sequences:

>Translated_493_residues
MVKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIG
LLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYK
IGLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISF
LAWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPY
ITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAW
AGFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKATMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHRE
FNEMEAVLEEETK
>Mature_493_residues
MVKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIG
LLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYK
IGLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISF
LAWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPY
ITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAW
AGFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKATMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHRE
FNEMEAVLEEETK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=494, Percent_Identity=55.0607287449393, Blast_Score=540, Evalue=1e-153,
Organism=Homo sapiens, GI11141885, Length=469, Percent_Identity=24.0938166311301, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI14140236, Length=539, Percent_Identity=23.3766233766234, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI110835708, Length=545, Percent_Identity=22.9357798165138, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI109659836, Length=381, Percent_Identity=22.5721784776903, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4507031, Length=470, Percent_Identity=24.2553191489362, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI109659839, Length=397, Percent_Identity=21.9143576826196, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI206597483, Length=464, Percent_Identity=23.0603448275862, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI206597487, Length=464, Percent_Identity=23.2758620689655, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787251, Length=489, Percent_Identity=49.079754601227, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI87082237, Length=460, Percent_Identity=29.3478260869565, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790503, Length=454, Percent_Identity=27.0925110132159, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1790113, Length=391, Percent_Identity=24.0409207161125, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539284, Length=447, Percent_Identity=25.2796420581655, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533094, Length=384, Percent_Identity=21.875, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648033, Length=458, Percent_Identity=24.235807860262, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356865, Length=458, Percent_Identity=24.235807860262, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221459584, Length=372, Percent_Identity=24.1935483870968, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640370, Length=358, Percent_Identity=23.7430167597765, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645928, Length=375, Percent_Identity=25.6, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281362918, Length=297, Percent_Identity=26.9360269360269, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651741, Length=466, Percent_Identity=24.8927038626609, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651739, Length=449, Percent_Identity=24.0534521158129, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459588, Length=380, Percent_Identity=23.1578947368421, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221459586, Length=385, Percent_Identity=22.0779220779221, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54006; Mature: 54006

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWML
CEEEHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEE
MGLPGAMYATGLSSVWIAIGLLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCHHHHHHHHHHH
TKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
AVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
LAWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TLQDPEMVFVTFSNILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRK
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRT
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NKWGVLAGMIVGALVVITWVQIPSLKATMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHRE
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNEMEAVLEEETK
HHHHHHHHHHCCC
>Mature Secondary Structure
MVKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWML
CEEEHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEE
MGLPGAMYATGLSSVWIAIGLLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDR
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCHHHHHHHHHHH
TKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
AVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
LAWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TLQDPEMVFVTFSNILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRK
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRT
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NKWGVLAGMIVGALVVITWVQIPSLKATMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHRE
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNEMEAVLEEETK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]