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The map label for this gene is vanS [H]

Identifier: 118478543

GI number: 118478543

Start: 3094509

End: 3095663

Strand: Reverse

Name: vanS [H]

Synonym: BALH_2919

Alternate gene names: 118478543

Gene position: 3095663-3094509 (Counterclockwise)

Preceding gene: 118478544

Following gene: 118478542

Centisome position: 58.89

GC content: 38.44

Gene sequence:

>1155_bases
TTGAAAAATAAAAATAAGAATAAAAAAATCGATTATTCAAAATTAAAACGGAAACTCTACCAATATATCCTTACGATTGT
TATGGCAGCAGTTGTATTTGTGCTGTTTTTACGTCTATTTATCCAAGGAACACTTGGAGAGTGGATCGTACGTTTTTTGG
AAAACAGTTATCATTTAGAGCGTTGGGACGCAATGATAATATATCAGTATACTATACGGAACAATATAGAAATTTTTATT
TATGTGGCGGTTGCCATTAGTATTCTTATTCTATGCCGAGTTATGCTTTTAAAATTTGTAAAATACTTTGAAGAGATAAA
TACCGGTATTGATATACTTATTCAGAACGTAGATAAACAAATTGAGCTTTCTGCGGAAATGGAGTTCATGGAACAGAAGC
TCAACACGTTAAAGCGAACCCTCGAAAAGCGAGAGCATGATGCAAAGCTGGCCGAACAAAGAAAAAATGAAGTTGTTATG
TACTTGGCACACGATATTAAAACGCCTCTTACATCAGTCATCGGTTATCTAATCCTGCTTGACGAGGCACCAGACATGCC
AAGAGAGCAAAAGGCAAAATATGTGCGTATCACGTTAGAAAAAGCGTATCGACTGGAACAGCTAATTGACGAGTTTTTTG
AAATTACGCGATATAACCTTCAAACGATTACGCTCACGAAAAAACATATAGATTTATATTATATGTTGGTGCAAATGACT
GATGAATTTTATCCTCAGCTTGCCGCGAAGGGAAAACAGGTGGTACTCCATGCTTCTGAAGATTTGACCGTATTTGGTGA
CCCTGATAAGCTAGCGAGGGTCTTTAACAATATTTTGAAAAATGCCACTGCATACAGCAAGGACGACAGCGTTATTGACA
TTAAGGCGGACCTATCCGAGGATGTGGTATCTATCGTTTTCGAGAACGCTGGATACATCCCGAAAGATAAACTCGCTACC
ATATTTGAAAAGTTTTACAGATTGGATGATGCCCGTTCTTCTGATACGGGCGGCGCGGGACTTGGATTGGCGATTGCAAA
AGAAATCATTGTTCAGCATGGTGGACAGATTTACGCGGAAAGTAATGATAACTCTACGACATTCACGGTAGAGCTTCCAG
CCTTTCAAGACTTGGTTGATAAAGAGGGTTCATAG

Upstream 100 bases:

>100_bases
AACACTATCACCGTGCATATCCGACATCTGCGAGAAAAGATGAACGACACGGTTGATAATCCGAAATATATAAAAACGGT
ATGGGGGATCGGCTATAAAA

Downstream 100 bases:

>100_bases
AAAATAATCATTGTTTCTTAAGGAATTCTTAAGGTTATCTCTACTTTTTCTTAGGAAATTAACAATTTAATATTAAAAAA
TGGCTCGTTCTTATGCGGTA

Product: vancomycin sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKNKNKNKKIDYSKLKRKLYQYILTIVMAAVVFVLFLRLFIQGTLGEWIVRFLENSYHLERWDAMIIYQYTIRNNIEIFI
YVAVAISILILCRVMLLKFVKYFEEINTGIDILIQNVDKQIELSAEMEFMEQKLNTLKRTLEKREHDAKLAEQRKNEVVM
YLAHDIKTPLTSVIGYLILLDEAPDMPREQKAKYVRITLEKAYRLEQLIDEFFEITRYNLQTITLTKKHIDLYYMLVQMT
DEFYPQLAAKGKQVVLHASEDLTVFGDPDKLARVFNNILKNATAYSKDDSVIDIKADLSEDVVSIVFENAGYIPKDKLAT
IFEKFYRLDDARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNSTTFTVELPAFQDLVDKEGS

Sequences:

>Translated_384_residues
MKNKNKNKKIDYSKLKRKLYQYILTIVMAAVVFVLFLRLFIQGTLGEWIVRFLENSYHLERWDAMIIYQYTIRNNIEIFI
YVAVAISILILCRVMLLKFVKYFEEINTGIDILIQNVDKQIELSAEMEFMEQKLNTLKRTLEKREHDAKLAEQRKNEVVM
YLAHDIKTPLTSVIGYLILLDEAPDMPREQKAKYVRITLEKAYRLEQLIDEFFEITRYNLQTITLTKKHIDLYYMLVQMT
DEFYPQLAAKGKQVVLHASEDLTVFGDPDKLARVFNNILKNATAYSKDDSVIDIKADLSEDVVSIVFENAGYIPKDKLAT
IFEKFYRLDDARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNSTTFTVELPAFQDLVDKEGS
>Mature_384_residues
MKNKNKNKKIDYSKLKRKLYQYILTIVMAAVVFVLFLRLFIQGTLGEWIVRFLENSYHLERWDAMIIYQYTIRNNIEIFI
YVAVAISILILCRVMLLKFVKYFEEINTGIDILIQNVDKQIELSAEMEFMEQKLNTLKRTLEKREHDAKLAEQRKNEVVM
YLAHDIKTPLTSVIGYLILLDEAPDMPREQKAKYVRITLEKAYRLEQLIDEFFEITRYNLQTITLTKKHIDLYYMLVQMT
DEFYPQLAAKGKQVVLHASEDLTVFGDPDKLARVFNNILKNATAYSKDDSVIDIKADLSEDVVSIVFENAGYIPKDKLAT
IFEKFYRLDDARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNSTTFTVELPAFQDLVDKEGS

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=255, Percent_Identity=29.4117647058824, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=27.5409836065574, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=25.0950570342205, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=29.718875502008, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790346, Length=201, Percent_Identity=27.8606965174129, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=237, Percent_Identity=26.1603375527426, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082128, Length=234, Percent_Identity=27.3504273504274, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=23.7704918032787, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=247, Percent_Identity=25.9109311740891, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789149, Length=283, Percent_Identity=24.7349823321555, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=276, Percent_Identity=25.3623188405797, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790551, Length=212, Percent_Identity=28.3018867924528, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787374, Length=203, Percent_Identity=28.0788177339901, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788279, Length=319, Percent_Identity=24.1379310344828, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44454; Mature: 44454

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKNKNKKIDYSKLKRKLYQYILTIVMAAVVFVLFLRLFIQGTLGEWIVRFLENSYHLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHH
RWDAMIIYQYTIRNNIEIFIYVAVAISILILCRVMLLKFVKYFEEINTGIDILIQNVDKQ
HHCEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
IELSAEMEFMEQKLNTLKRTLEKREHDAKLAEQRKNEVVMYLAHDIKTPLTSVIGYLILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHEE
DEAPDMPREQKAKYVRITLEKAYRLEQLIDEFFEITRYNLQTITLTKKHIDLYYMLVQMT
CCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHH
DEFYPQLAAKGKQVVLHASEDLTVFGDPDKLARVFNNILKNATAYSKDDSVIDIKADLSE
HHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHH
DVVSIVFENAGYIPKDKLATIFEKFYRLDDARSSDTGGAGLGLAIAKEIIVQHGGQIYAE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
SNDNSTTFTVELPAFQDLVDKEGS
CCCCCEEEEEECCHHHHHHCCCCC
>Mature Secondary Structure
MKNKNKNKKIDYSKLKRKLYQYILTIVMAAVVFVLFLRLFIQGTLGEWIVRFLENSYHLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHH
RWDAMIIYQYTIRNNIEIFIYVAVAISILILCRVMLLKFVKYFEEINTGIDILIQNVDKQ
HHCEEEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHH
IELSAEMEFMEQKLNTLKRTLEKREHDAKLAEQRKNEVVMYLAHDIKTPLTSVIGYLILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHEE
DEAPDMPREQKAKYVRITLEKAYRLEQLIDEFFEITRYNLQTITLTKKHIDLYYMLVQMT
CCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHH
DEFYPQLAAKGKQVVLHASEDLTVFGDPDKLARVFNNILKNATAYSKDDSVIDIKADLSE
HHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHH
DVVSIVFENAGYIPKDKLATIFEKFYRLDDARSSDTGGAGLGLAIAKEIIVQHGGQIYAE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
SNDNSTTFTVELPAFQDLVDKEGS
CCCCCEEEEEECCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]