Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 118476851

GI number: 118476851

Start: 1294619

End: 1295950

Strand: Direct

Name: dacF [H]

Synonym: BALH_1139

Alternate gene names: 118476851

Gene position: 1294619-1295950 (Clockwise)

Preceding gene: 118476850

Following gene: 118476852

Centisome position: 24.63

GC content: 36.04

Gene sequence:

>1332_bases
ATGCAGTTTTTAAGCTGTGAGAGTATAGTGAAAAGGGAGAGTCATATGGTAAATTTTAAGAAAATTTTGGCTTTTATTAC
CGTTATTTGTTTATTTTTTTTGACACCTATGACGTTACGTGCTGAGACGAATATTGGGGTGAACCCAGAACAAGTGGCAC
CACCACCTAAGGAAGGGCCAAATGTTTTTAGTCAGTTTGCAACTACAATTGATGCGAAAACTGGAGATATTTTGTATGAT
AAAAACGCACATCACCGTGCATTTCCGGCTAGTATGACGAAAGTGTTAACGGCGATTTTACTTATGGAACATACGAAGCC
AGAGGATCAATTTACATTTTCACAATTAGCATTAGATCAAGAGAAGAGTAATTATCAAATTGAGTTTCAACCTGGTGAGA
CGATTAATAGAAATACTGCACTTATGATTTTAATGGTGCTGAGTGCAAATGATGTGTCGTATGCGATTGCGGAGCGTATT
GGCGGAAGTGTTGAGAATTTCGCTAATATGATGAATGAGAGAGCGAAGCAATTAGGCGCTACCGACAGTCATTTTGTAAC
ACCAAACGGATTGCATGATCCGAATCATTATACTACGCCATATGATATGGCTATGATTACGAGAGGTGTGCAAAAGTATC
CTGAAATTTTACAAGCGATGAATACGAAAAGAACAACAGTTACGACATCTAGGCAGACGGTGTCTATTTTTAATAAATCT
ACTTATTTTGAAAATCCATTTAGTATTGGTGGTAAGACAGGTTTTACAAATGAAGCGCGCAATACACTCGTTTTATTAAA
TGAGAAGGATGGAAATCGTATTATAAACGTAGTAATGGCTTCTCAAAGACCTGAAATTTATGAAGATTTAAAGCAGATGG
CGGACTATTCTTTCGGTCAATTTGTGAAGCAAACTGTACTGGATAAACATAGTTGGCATCAAAAGACAACGTATTTAAAT
AAAGATATTGATAGTGAGCTTGAAAAAAGTGCAGAACTAATGCTTAAGAAAGATGAAGGGAAAAATGTGAAGACGACGTT
CCGGGCGGCTTCATTAGATAAAGATTCTTTATATCATAAAGGAATTCATCGTGGTGAAGTAGTTGGTGCAGTTGATATTA
CGAAAAATAATCAAACAATTGAGACGGTAAATGTTCTTTCTAAAGAAGATGTTACGTTTGCGATGCCTAAAAAAGATACG
AACGAGCCTGAAGTAAACGAATCAAACGTGAAAATAATAAGCATTGGAATAGGTGCTGCCATATTATTTGGAGCCATTTT
ATTTGTAGTGCTACGCCGAAATACAAAAGGAATGAAATCAGAAGAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTTATTGTATATTTATGTTCTTCATCGTAAAAGTATGATATAGTAAAAAACAGTGAATGTTCTTCCAATTTAATACA
AATTACATATAGATTGGTAG

Downstream 100 bases:

>100_bases
ATTACTAGTTTAAAAGGACTGTGAAGAGACTTCTTCACAGTCCTTTTTTTATATTGAAAATAACTAATAATTGTAAATTT
TCTTATTGCATTTCTAATAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MQFLSCESIVKRESHMVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYD
KNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERI
GGSVENFANMMNERAKQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKS
TYFENPFSIGGKTGFTNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLN
KDIDSELEKSAELMLKKDEGKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIETVNVLSKEDVTFAMPKKDT
NEPEVNESNVKIISIGIGAAILFGAILFVVLRRNTKGMKSEEK

Sequences:

>Translated_443_residues
MQFLSCESIVKRESHMVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYD
KNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERI
GGSVENFANMMNERAKQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKS
TYFENPFSIGGKTGFTNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLN
KDIDSELEKSAELMLKKDEGKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIETVNVLSKEDVTFAMPKKDT
NEPEVNESNVKIISIGIGAAILFGAILFVVLRRNTKGMKSEEK
>Mature_443_residues
MQFLSCESIVKRESHMVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGPNVFSQFATTIDAKTGDILYD
KNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERI
GGSVENFANMMNERAKQLGATDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKS
TYFENPFSIGGKTGFTNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQFVKQTVLDKHSWHQKTTYLN
KDIDSELEKSAELMLKKDEGKNVKTTFRAASLDKDSLYHKGIHRGEVVGAVDITKNNQTIETVNVLSKEDVTFAMPKKDT
NEPEVNESNVKIISIGIGAAILFGAILFVVLRRNTKGMKSEEK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=24.5333333333333, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87082022, Length=340, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786851, Length=376, Percent_Identity=24.2021276595745, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49814; Mature: 49814

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLSCESIVKRESHMVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCH
NVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQ
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
EKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGA
CCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
TDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKS
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCC
TYFENPFSIGGKTGFTNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQ
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHH
FVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELMLKKDEGKNVKTTFRAASLDKDSLYHK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEHHHHCCCHHHHHHC
GIHRGEVVGAVDITKNNQTIETVNVLSKEDVTFAMPKKDTNEPEVNESNVKIISIGIGAA
CCCCCCEEEEEEEECCCCEEHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEECHHHH
ILFGAILFVVLRRNTKGMKSEEK
HHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQFLSCESIVKRESHMVNFKKILAFITVICLFFLTPMTLRAETNIGVNPEQVAPPPKEGP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCH
NVFSQFATTIDAKTGDILYDKNAHHRAFPASMTKVLTAILLMEHTKPEDQFTFSQLALDQ
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
EKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNERAKQLGA
CCCCEEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
TDSHFVTPNGLHDPNHYTTPYDMAMITRGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKS
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHCCC
TYFENPFSIGGKTGFTNEARNTLVLLNEKDGNRIINVVMASQRPEIYEDLKQMADYSFGQ
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHH
FVKQTVLDKHSWHQKTTYLNKDIDSELEKSAELMLKKDEGKNVKTTFRAASLDKDSLYHK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEHHHHCCCHHHHHHC
GIHRGEVVGAVDITKNNQTIETVNVLSKEDVTFAMPKKDTNEPEVNESNVKIISIGIGAA
CCCCCCEEEEEEEECCCCEEHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEECHHHH
ILFGAILFVVLRRNTKGMKSEEK
HHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]