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The map label for this gene is pbpF [H]

Identifier: 118476671

GI number: 118476671

Start: 1088302

End: 1090530

Strand: Direct

Name: pbpF [H]

Synonym: BALH_0949

Alternate gene names: 118476671

Gene position: 1088302-1090530 (Clockwise)

Preceding gene: 118476669

Following gene: 118476672

Centisome position: 20.7

GC content: 38.99

Gene sequence:

>2229_bases
TTGTTTGTTTCTATTATATCAACTCTCTTAAAAAACTTCGAAGATTATATACATAAAGATACTTCTTTTTCAAATAATAA
ATGTACCATCATTTGGAAAGAAGGCCGATGTATGAAGAAAGTAAAGTGGACTTTATTAGGTGGGTTAGCAACGCTTGTAT
TAGCGATTGTACTTTATAAACTTATCGTGTTAGCTGGTGGCTATATGATGGATGAAAAACAGCTCGTTTTCCACTCTTCA
TCACGTATCGTTGACCAGAAAGGGAAAGAAATTACGAAATTATACGTAGAAAATAGAGACCTCGTACCAATCGAACAAAT
TCCAAAATATGTGCAGCAGGCGTTTGTTGCGGTAGAGGATTCTCGTTTTTATGAGCATCAAGGAATCGATTATCCGTCTA
TATTCCGCGCTCTGTATAAAGACACGTTAGCTGGAGAGAAGGTCGAAGGCGGCAGTACGATTACGCAGCAACTCGCTAAA
AATGTTTTTTTAACTCGTGAAAAAACAGTTACGCGTAAATTGAAGGAAGTCGCAATTTCCCTTCAATTAGAGCAAAAATA
TACGAAGCAACAAATTCTTGAAATGTATATGAATCATATTTATTTTGGCCATGGGGCTTACGGTATTCAAGCTGCAGCAA
GGTTGTATTTTAATAAAAATGTAGAAGATTTAACAGTAGAAGAAGGGGCAATGCTTGCAGGTCTTCCGAAGTCGCCAAAC
GGATATTCACCTTATTTCTCTCCAGAGAAAAGTAAGGAGCGTCGCGATCTCGTATTGTCACTTATGCATAAACAAGGCTA
TTTAACTGCCGAAGAAAGCGTGCGTTATCAAGGAAAAACAATTGCACTATATAAAAACTTAGATGAGAATGAGCTCGCAT
ATATGCCGTATATTGATATGGTCATAGATGAAGCGGCTCGTTTATACGGGTTGTCTCATCAAGAAGTACTCCGCGGAGGA
TATACGTTTGTCGTACCGATGGATGAAAAAATTCAAAAGGTAGCGTATAACCAGTTTCAAGATGCAAGGAATTTCCCTGG
GAAAGAAGAGGGGGCGCAAGGTGCATTTTTATTAATGGATAATCGTACGGGCGGGATTAAAGCGGCGATTGGCGGAAGAA
AGTACGTCCCGAGAGGATTTAATCGTGTTTTTGCGAAAAGACAACCTGGTTCTGTACTAAAACCACTGATCGTCTATGCA
CCAGCACTCGAAACGAAAAAGTATAATCCGTATTCTTTATTGACGAATGAAAGACATTCTTTTGAAGGGTATGAACCTCG
AAACTATAACCGTGAATATTCAAAAGAAATAACGATGTACGATGCGATTTTAGAGTCAGCAAATGTACCGGCAGTTTCTT
TATTAAACGAGTTGGGGGTAGAAGAAGGAAAGCAATATTTAGAGAAAGGAAATGTTCACATTGCCGATGCTGGTTTAAGC
ACAGCGCTTGGCGGATTGAAAAATGGTGTTTCAACATTTGATCTTGTGAAAATGTATCGTGCGTTTTTAGCAAATGGTGA
TATTATTGAGCCGCACGTTATTGATAAAGTGTTAAATAGACACGGCGCAGTCATTGGAGAATCTCCAAAAGTAGAAACGA
AAATTTTCTCGAAACAAACGGCATGGTATATGACGAAAATGTTAGAAGGAGTTGTAAAGGAAGGGACAGCGAAAGCCGGT
GTATATAGCGGAGCGTTAGCTGGGAAAACAGGTACAACTTCCTTACCAAATGACGATAATGGGGCGAGAGATATGTGGTT
CGTTGGCTATACGCCAAATTTAGTAGGCGCTGTTTGGATTGGTTATGACCGCATAGATAAAGAGCATAAGTTACAAGGAG
AAAGTGCGTCAGCAACAAAATTGTTTAAGAAAATTTTAACGAAAGCAAATGTAGAACAGAAAGAGCAGTTTAGGAAACCA
GAAGGTGTGGAAACAATCGGTGCTCCAATTCGGCTGCGCAAGATTGAAGATGTGAAAATGAAATTAGCGTTTAGCCCTTT
CGGTTTATTTAAAGCGAAATTAAGCTGGACACCACTTCCAGATGAAAGAATTATGTATCGTATTTATAGAGTGGAAAACG
GAATTCATACGCACGTAGGTACTGTAAACGGTGCCGGGGAATATGAGGAAAAGTTCGTTAACATCTTTTCAAAACCGAGT
TTTTACGTTGTGCCATATAACACACAAACGAATCGTGAAGGAGAAAAGTCAAAAGTAGCTAAACCATAG

Upstream 100 bases:

>100_bases
TTTATAAAACATATCTTTTTCATTATTTTTTGCAGTGAAATGTGCTTGTTCTAGAGGTGTTTCGTATGAAATAATAGCCT
TCATTTTAATGCCCCCTTAA

Downstream 100 bases:

>100_bases
TTTTTCTCTGTGTTATAATAAGAATGTTGTGAAAATAGTAAGCGTTTTTACGAATGTTTGTGTCAATTTAATGAACAACG
TACATAATATTATGCATTTT

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MFVSIISTLLKNFEDYIHKDTSFSNNKCTIIWKEGRCMKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSS
SRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAK
NVFLTREKTVTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAARLYFNKNVEDLTVEEGAMLAGLPKSPN
GYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQGKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGG
YTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYA
PALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLS
TALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKVLNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAG
VYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKP
EGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPS
FYVVPYNTQTNREGEKSKVAKP

Sequences:

>Translated_742_residues
MFVSIISTLLKNFEDYIHKDTSFSNNKCTIIWKEGRCMKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSS
SRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAK
NVFLTREKTVTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAARLYFNKNVEDLTVEEGAMLAGLPKSPN
GYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQGKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGG
YTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYA
PALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLS
TALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKVLNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAG
VYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKP
EGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPS
FYVVPYNTQTNREGEKSKVAKP
>Mature_742_residues
MFVSIISTLLKNFEDYIHKDTSFSNNKCTIIWKEGRCMKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSS
SRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAK
NVFLTREKTVTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAARLYFNKNVEDLTVEEGAMLAGLPKSPN
GYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQGKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGG
YTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYA
PALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLS
TALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKVLNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAG
VYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKP
EGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPS
FYVVPYNTQTNREGEKSKVAKP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=304, Percent_Identity=38.8157894736842, Blast_Score=195, Evalue=1e-50,
Organism=Escherichia coli, GI1786343, Length=566, Percent_Identity=28.7985865724382, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788867, Length=537, Percent_Identity=26.8156424581006, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=35.4651162790698, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 83789; Mature: 83789

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSIISTLLKNFEDYIHKDTSFSNNKCTIIWKEGRCMKKVKWTLLGGLATLVLAIVLYK
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVAVED
HHHHHCCCEECCCCEEEECCCHHHHHCCCCEEHHEECCCCCCCHHHHHHHHHHHHHHHHC
SRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTVTRKLKEVAIS
CHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAARLYFNKNVEDLTVEEGAMLAGLPKSPN
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCC
GYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQGKTIALYKNLDENELAYMPYIDM
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCCCCEEEEEECCCCCCEEECCHHHH
VIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFLLMD
HHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHS
CCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
FEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCEEEECCCHH
TALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKVLNRHGAVIGESPKVETKIFSKQT
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHH
AWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGAVWI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEE
GYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKM
CHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHCCCCEEEEEECCEEE
KLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPS
EEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCC
FYVVPYNTQTNREGEKSKVAKP
EEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFVSIISTLLKNFEDYIHKDTSFSNNKCTIIWKEGRCMKKVKWTLLGGLATLVLAIVLYK
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVAVED
HHHHHCCCEECCCCEEEECCCHHHHHCCCCEEHHEECCCCCCCHHHHHHHHHHHHHHHHC
SRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTVTRKLKEVAIS
CHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAARLYFNKNVEDLTVEEGAMLAGLPKSPN
HHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCC
GYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQGKTIALYKNLDENELAYMPYIDM
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCCCCEEEEEECCCCCCEEECCHHHH
VIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFLLMD
HHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHS
CCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
FEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCEEEECCCHH
TALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKVLNRHGAVIGESPKVETKIFSKQT
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHH
AWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGAVWI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEE
GYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKM
CHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHCCCCEEEEEECCEEE
KLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPS
EEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCC
FYVVPYNTQTNREGEKSKVAKP
EEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]