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The map label for this gene is napB [H]

Identifier: 117921870

GI number: 117921870

Start: 4110083

End: 4110514

Strand: Direct

Name: napB [H]

Synonym: Shewana3_3433

Alternate gene names: 117921870

Gene position: 4110083-4110514 (Clockwise)

Preceding gene: 117921869

Following gene: 117921872

Centisome position: 82.66

GC content: 50.93

Gene sequence:

>432_bases
ATGAAAAAAATACTCACCTTAGCTGCGATTGTATTAGCCATCGGCGGCTGCTCAGGCCAACAAGCAGACTCTCAAACCAC
ACCCGTAAACATCAAATCCCTCGCCGGCGATAGCGCGGTAACGGATATTCGCCCAGCCGATGCCATGCCCGTCTACCCAA
GCCGTGGCAAGGCGCTAGAGCGCAGCTTTACCGACCAACCACCACTCATTCCCCATAAGGATGATTACAAAATCACCTTG
GATAAGAATGGCTGTTTAACTTGTCACAGCTGGGATAAAGCCCCACGGATGAAAGCCACGCCAGTGGCGAAGTCCCACGT
AATCGACGATAAAGGCACGCTCAATGGCCATAACTATTTCTGCACCCAGTGCCACGTTGCACAGGCAGAAAACAAGGCGC
CTTTAGTCGAGAACAAGTTCTCAAATCAATAA

Upstream 100 bases:

>100_bases
CTCAGGCTCAACTTAGCAAAGGCGATTCGACCGATTGTCAGTCGCTATCAACCACTTCACCAATTCGTCAACACATTGCC
CCCAAGGCGGAGAATGACCA

Downstream 100 bases:

>100_bases
CACAGCACTTCCCTGCCCACAAAAGCCACTGCTAACCCAGTGGCTTTTTATTTTCCGCAATAAAATCTCAGCGCTAAGCT
TTGCCCGCATAGTGTTCAAA

Product: periplasmic nitrate reductase subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKKILTLAAIVLAIGGCSGQQADSQTTPVNIKSLAGDSAVTDIRPADAMPVYPSRGKALERSFTDQPPLIPHKDDYKITL
DKNGCLTCHSWDKAPRMKATPVAKSHVIDDKGTLNGHNYFCTQCHVAQAENKAPLVENKFSNQ

Sequences:

>Translated_143_residues
MKKILTLAAIVLAIGGCSGQQADSQTTPVNIKSLAGDSAVTDIRPADAMPVYPSRGKALERSFTDQPPLIPHKDDYKITL
DKNGCLTCHSWDKAPRMKATPVAKSHVIDDKGTLNGHNYFCTQCHVAQAENKAPLVENKFSNQ
>Mature_143_residues
MKKILTLAAIVLAIGGCSGQQADSQTTPVNIKSLAGDSAVTDIRPADAMPVYPSRGKALERSFTDQPPLIPHKDDYKITL
DKNGCLTCHSWDKAPRMKATPVAKSHVIDDKGTLNGHNYFCTQCHVAQAENKAPLVENKFSNQ

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=94, Percent_Identity=34.0425531914894, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15489; Mature: 15489

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILTLAAIVLAIGGCSGQQADSQTTPVNIKSLAGDSAVTDIRPADAMPVYPSRGKALE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
RSFTDQPPLIPHKDDYKITLDKNGCLTCHSWDKAPRMKATPVAKSHVIDDKGTLNGHNYF
HCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKAPLVENKFSNQ
EEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKILTLAAIVLAIGGCSGQQADSQTTPVNIKSLAGDSAVTDIRPADAMPVYPSRGKALE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
RSFTDQPPLIPHKDDYKITLDKNGCLTCHSWDKAPRMKATPVAKSHVIDDKGTLNGHNYF
HCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKAPLVENKFSNQ
EEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]