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The map label for this gene is ispB [H]

Identifier: 117921658

GI number: 117921658

Start: 3862811

End: 3863806

Strand: Direct

Name: ispB [H]

Synonym: Shewana3_3220

Alternate gene names: 117921658

Gene position: 3862811-3863806 (Clockwise)

Preceding gene: 117921650

Following gene: 117921661

Centisome position: 77.69

GC content: 48.8

Gene sequence:

>996_bases
ATGGCCCCACAACCAGAGTCTATTATGGATTTAAACGCTATTCGTCAACTGGCTGACACTGATATGCAAGCAGTCAATCA
GCTGATCTATAAACAGCTGGAGTCAGATGTCGCCCTGATAAACCAGTTAGGTTTCTACATTATTAATGGTGGTGGTAAAC
GTCTTCGTCCTTTACTGTCCGTCCTCGCAGCACGCGCCGTTGACTACCAAGGTGAAGCTCACCTTAAGCTTGCCGCGATT
ATTGAATTTATTCATACCGCTTCATTACTGCACGATGATGTGGTCGATGAATCGACCCTCAGACGCGGCCGCGAAACCGC
CAACGCACTCTTTGGTAACAGCGCCAGTGTATTAGTGGGAGACTTCCTTTATACCCGCTCATTCCAAATGATGACCGAAC
TCGACAGCATGCGCGTACTGCGTGTATTAGCCGATACCACCAACGTATTGGCCGAGGGGGAAGTATTACAGCTAATGAAC
TGTAATGACCCTAATACCACAGAAGAAAGCTATATGCGGGTGATCTATTGTAAGACCGCTAAGCTATTCGAAGCCGCCAC
GTTACTGGCTGCCGTATTAGCCGGCGCCACTCCCGAGCAAGAGGCCGCATTAGGTGACTATGGTAAGTATTTAGGTACTG
CCTTCCAGTTAACAGATGATTTACTCGACTACACCGCCGACAGTGAAGAGTTAGGTAAAAACATCGGTGATGACCTCGCC
GAAGGTAAACCGACCTTACCGCTGATCTATGCCATTGCCCATGGCACAGAAGAAGAGCAGCAACTCATTCGTCAAGCGAT
TGAGCAAGGTGATGGCACCCACGCCATTGAGAAAATTGTTGCCGCTCTGCATCACTGCGGCGCGCTCACTTACACTCAGC
AAAAAGCCATTGAAGAATCCAACAAGGCGATTGCAGCGCTGGCGGTATTACCCGACAGTGATTTCAAAACCGCATTAATT
TCGCTGGCAAAAATCTCCGTTGATCGCAACCACTAA

Upstream 100 bases:

>100_bases
TAACTCGCACATTAGTAGTGTGAGTACTGTCATATGTCGGAATTTTAGGTAAAATTCATTCTATATAACACGTGAGCCTA
AAATAGAGGCCGGGTACGTA

Downstream 100 bases:

>100_bases
TTGGCTGCGATAAACCAATAAAAAGGCCTTGCTTAGCAAGGCCTTTTTATTTTTAACGTTCTTTATCAAAGCGAACTTAA
ACCTGCCATTGGATCGGTAC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MAPQPESIMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVDYQGEAHLKLAAI
IEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMRVLRVLADTTNVLAEGEVLQLMN
CNDPNTTEESYMRVIYCKTAKLFEAATLLAAVLAGATPEQEAALGDYGKYLGTAFQLTDDLLDYTADSEELGKNIGDDLA
EGKPTLPLIYAIAHGTEEEQQLIRQAIEQGDGTHAIEKIVAALHHCGALTYTQQKAIEESNKAIAALAVLPDSDFKTALI
SLAKISVDRNH

Sequences:

>Translated_331_residues
MAPQPESIMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVDYQGEAHLKLAAI
IEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMRVLRVLADTTNVLAEGEVLQLMN
CNDPNTTEESYMRVIYCKTAKLFEAATLLAAVLAGATPEQEAALGDYGKYLGTAFQLTDDLLDYTADSEELGKNIGDDLA
EGKPTLPLIYAIAHGTEEEQQLIRQAIEQGDGTHAIEKIVAALHHCGALTYTQQKAIEESNKAIAALAVLPDSDFKTALI
SLAKISVDRNH
>Mature_330_residues
APQPESIMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSVLAARAVDYQGEAHLKLAAII
EFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVGDFLYTRSFQMMTELDSMRVLRVLADTTNVLAEGEVLQLMNC
NDPNTTEESYMRVIYCKTAKLFEAATLLAAVLAGATPEQEAALGDYGKYLGTAFQLTDDLLDYTADSEELGKNIGDDLAE
GKPTLPLIYAIAHGTEEEQQLIRQAIEQGDGTHAIEKIVAALHHCGALTYTQQKAIEESNKAIAALAVLPDSDFKTALIS
LAKISVDRNH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=30.4347826086957, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI4758430, Length=262, Percent_Identity=27.4809160305344, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI83700220, Length=262, Percent_Identity=27.4809160305344, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=64.9068322981366, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=30.6382978723404, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=321, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=289, Percent_Identity=33.5640138408304, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325188, Length=244, Percent_Identity=25.8196721311475, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.5436241610738, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281365769, Length=297, Percent_Identity=26.2626262626263, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24660002, Length=297, Percent_Identity=26.2626262626263, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36096; Mature: 35965

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPQPESIMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
VLAARAVDYQGEAHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
DFLYTRSFQMMTELDSMRVLRVLADTTNVLAEGEVLQLMNCNDPNTTEESYMRVIYCKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEEHEEHHH
KLFEAATLLAAVLAGATPEQEAALGDYGKYLGTAFQLTDDLLDYTADSEELGKNIGDDLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHH
EGKPTLPLIYAIAHGTEEEQQLIRQAIEQGDGTHAIEKIVAALHHCGALTYTQQKAIEES
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NKAIAALAVLPDSDFKTALISLAKISVDRNH
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
APQPESIMDLNAIRQLADTDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
VLAARAVDYQGEAHLKLAAIIEFIHTASLLHDDVVDESTLRRGRETANALFGNSASVLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
DFLYTRSFQMMTELDSMRVLRVLADTTNVLAEGEVLQLMNCNDPNTTEESYMRVIYCKTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEEHEEHHH
KLFEAATLLAAVLAGATPEQEAALGDYGKYLGTAFQLTDDLLDYTADSEELGKNIGDDLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHH
EGKPTLPLIYAIAHGTEEEQQLIRQAIEQGDGTHAIEKIVAALHHCGALTYTQQKAIEES
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHC
NKAIAALAVLPDSDFKTALISLAKISVDRNH
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]