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The map label for this gene is ksgA [H]

Identifier: 117921647

GI number: 117921647

Start: 3853650

End: 3854456

Strand: Direct

Name: ksgA [H]

Synonym: Shewana3_3209

Alternate gene names: 117921647

Gene position: 3853650-3854456 (Clockwise)

Preceding gene: 117921646

Following gene: 117921648

Centisome position: 77.5

GC content: 49.69

Gene sequence:

>807_bases
ATGAGTAGCAAAGTACATTTAGGCCACACGGCCAGAAAGCGTTTCGGACAAAACTTTTTAACCGACGACAATGTGATTAA
CCGCATTGTGGGTGCGATCGTCCCCGATAATGACCACGTGATGGTCGAAATCGGCCCCGGCCTTGGCGCCTTAACCGAAC
CTGTCGCCACGGCGATAGATAACCTGACCGTTGTCGAGTTAGACCGCGATCTGGTTGAGCGCTTGCAGAATCACCCTGTA
CTTAAGGATAAGCTCACGATTCACCAAGGCGATGCGCTGCAATTCGACTTTAGCCAGTTGGTGGTACCGGGTAAAAAACT
CAAAGTGTTTGGTAACTTACCCTATAACATTTCAACGCCTTTGATGTTCCATCTGTTCGAGTTTGCCGAGCAGATTGAAA
CCATGCACTTTATGTTGCAAAAAGAAGTGGTACTGCGTTTATCGGCAAGCCCGGGTAACAAGGCCTATGGCCGCTTAACG
GTGATGGCACAGTACTTCTGCCAAGTGGTTCCCGTGCTCGAAGTGCCACCCCATAGCTTTGCGCCACCGCCAAAGGTGGA
TTCTGCCGTGGTACGTCTACTGCCCTATGCAGAAAAACCTTTCCCCTGTAAGGATGTGAATGTACTGCGCCAGCTGTGCA
CCACAGCCTTTAACATGCGCCGTAAAACACTGCGTAACAACCTCAAGCAAGTGTTAAGCGATGAAGAGTTTGAGCAATTA
GGCATAGATCCCAATCTGCGCCCTGAGCAAATCAGTGTTGAGCAATATGTCGCCATGGCGAATATGGTGTGTGATAAGCA
GGCTTAA

Upstream 100 bases:

>100_bases
TCGATCTTGCGGGAACTGGCAAAGCCGATATTGGCAGTTTTGTCTGCGCCTTGAATAAAGCCATTGAGTTATCCTCAAAA
AACTAAACGATTAGATATTA

Downstream 100 bases:

>100_bases
GCAATATCAGTTTGATGGGCACTTTAGGGTGCCCTATCTTATTCAAGGGAGCAACATGAGCGCATTGGATGACTCAATCC
GAGTCGAAGTCAAAACCGAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSSKVHLGHTARKRFGQNFLTDDNVINRIVGAIVPDNDHVMVEIGPGLGALTEPVATAIDNLTVVELDRDLVERLQNHPV
LKDKLTIHQGDALQFDFSQLVVPGKKLKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGNKAYGRLT
VMAQYFCQVVPVLEVPPHSFAPPPKVDSAVVRLLPYAEKPFPCKDVNVLRQLCTTAFNMRRKTLRNNLKQVLSDEEFEQL
GIDPNLRPEQISVEQYVAMANMVCDKQA

Sequences:

>Translated_268_residues
MSSKVHLGHTARKRFGQNFLTDDNVINRIVGAIVPDNDHVMVEIGPGLGALTEPVATAIDNLTVVELDRDLVERLQNHPV
LKDKLTIHQGDALQFDFSQLVVPGKKLKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGNKAYGRLT
VMAQYFCQVVPVLEVPPHSFAPPPKVDSAVVRLLPYAEKPFPCKDVNVLRQLCTTAFNMRRKTLRNNLKQVLSDEEFEQL
GIDPNLRPEQISVEQYVAMANMVCDKQA
>Mature_267_residues
SSKVHLGHTARKRFGQNFLTDDNVINRIVGAIVPDNDHVMVEIGPGLGALTEPVATAIDNLTVVELDRDLVERLQNHPVL
KDKLTIHQGDALQFDFSQLVVPGKKLKVFGNLPYNISTPLMFHLFEFAEQIETMHFMLQKEVVLRLSASPGNKAYGRLTV
MAQYFCQVVPVLEVPPHSFAPPPKVDSAVVRLLPYAEKPFPCKDVNVLRQLCTTAFNMRRKTLRNNLKQVLSDEEFEQLG
IDPNLRPEQISVEQYVAMANMVCDKQA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=293, Percent_Identity=31.0580204778157, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI7657198, Length=268, Percent_Identity=30.9701492537313, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=62.781954887218, Blast_Score=341, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI25141369, Length=287, Percent_Identity=28.9198606271777, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=32.3943661971831, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=219, Percent_Identity=31.9634703196347, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357273, Length=283, Percent_Identity=28.9752650176678, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30157; Mature: 30026

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKVHLGHTARKRFGQNFLTDDNVINRIVGAIVPDNDHVMVEIGPGLGALTEPVATAID
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC
NLTVVELDRDLVERLQNHPVLKDKLTIHQGDALQFDFSQLVVPGKKLKVFGNLPYNISTP
CCEEEECCHHHHHHHHCCCCCHHCEEEECCCCEEECHHHHHCCCCEEEEEECCCCCCCHH
LMFHLFEFAEQIETMHFMLQKEVVLRLSASPGNKAYGRLTVMAQYFCQVVPVLEVPPHSF
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCC
APPPKVDSAVVRLLPYAEKPFPCKDVNVLRQLCTTAFNMRRKTLRNNLKQVLSDEEFEQL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GIDPNLRPEQISVEQYVAMANMVCDKQA
CCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSKVHLGHTARKRFGQNFLTDDNVINRIVGAIVPDNDHVMVEIGPGLGALTEPVATAID
CCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC
NLTVVELDRDLVERLQNHPVLKDKLTIHQGDALQFDFSQLVVPGKKLKVFGNLPYNISTP
CCEEEECCHHHHHHHHCCCCCHHCEEEECCCCEEECHHHHHCCCCEEEEEECCCCCCCHH
LMFHLFEFAEQIETMHFMLQKEVVLRLSASPGNKAYGRLTVMAQYFCQVVPVLEVPPHSF
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCC
APPPKVDSAVVRLLPYAEKPFPCKDVNVLRQLCTTAFNMRRKTLRNNLKQVLSDEEFEQL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GIDPNLRPEQISVEQYVAMANMVCDKQA
CCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA