Click here to switch to the map view.

The map label for this gene is yaaJ [H]

Identifier: 117921582

GI number: 117921582

Start: 3769478

End: 3770944

Strand: Reverse

Name: yaaJ [H]

Synonym: Shewana3_3144

Alternate gene names: 117921582

Gene position: 3770944-3769478 (Counterclockwise)

Preceding gene: 117921588

Following gene: 117921581

Centisome position: 75.84

GC content: 48.81

Gene sequence:

>1467_bases
ATGCTAGAAACGATTGTAAACTTTTTGAACGCACTGCTCTGGGGCAAATTGCTCGTTTACGGATTGGTGGGTGCGGGGCT
TTATTTCACCTTACGACTTGTATTTATTCAACTTACACACTTCAAGCACTCTCTAAAAGTCATGACCATGAGCCGCCAAG
GCTGTGAAAGTGGTCTGTCTTCATTCCAAGTCTTTTGTACCAGTATGGCCGCTCGGGTTGGTGCCGGAAACATGGCTGGT
GTTGCCGTGGCGATTGGCGCTGCTGGACCTGGCGCTGTGTTCTGGATGTGGTTAATCGCCATGTTGGGTATGGCGACGGC
TATGGTGGAGTCGACGCTTGCACAAGTATATAAGGTTCGAGACACCAACGGTCAGTTTCGAGGCGGTCCTTCCTATTATA
TGGAGAAGGGACTTGGTCAGAGGTGGATGGGGGTGTTGTTCGCTTTCTTCCTGATCATCGCCTTTGGTTTAGTGTTTAAC
GCGGTGCAAGCCAATACCATTACAGGCGCAATGGAGCGGGTGTTTGGCTTCAACCCGACCTATGTTGGTATCGGCTTAGT
GTTAGCCAGTGGTTTTGTGATTGTCGGTGGCTTACGTAAAGTTGCGAGAGTATCAGAGTTTATCGTGCCTATCATGGCAC
TGGCTTATATTCTGATCGCTTTTATTATCGTGCTGTTCAATTTAGATCAACTGCCAGCCATCATCAGCTTGATTGTAAAG
AGTGCGTTCGGTTGGCAAGAGGCCGCCGCAGGTGGTGTTGCTTATACGGTCGCTCAGGCTATGCAAGCGGGAATCGCCCG
TGGTTTGTTTTCAAACGAGGCGGGGATGGGCAGCGCGGCAAACGTGGCGGCGAGTGCCTCACCTAATCCTAATCATCCTG
CATCCCAAGGTTTTGTGCAGATGATGGGAGTGTTTGTCGATACCATCGTTATCTGTACAGCAACTGCGGCGATTATTTTG
CTTTCAGGCGATATCGGTTCTAGCGAAGATGGCATTCGTCTCACCATCAATGCGATGTCAAACCACGTAGGAGATTGGGG
CGGCGCCTTTATTGCGATAGCTATCTTCCTATTCTGCTTTACCTCTATCATTGCTAACTATTCCTACGCCGAAACCAACG
TAATGTTCTTAACGGGTAACAGCACTAAGGCGTTACCGCTATTTCGTCTGTGCGTATTAGGCATGGTGATGTTTGGTGCT
GTGGCCAAAATCAGCTTAGTGTGGAACTTAGCGGACGTGTCTATGGGACTAATGGCAACGGTCAATATCATTGCATTGCT
GCTGTTATCTGGCTTAGCAATACGGGTTATCAACGATTACTGTGAGCAATTGAAGAGTGGCAAAATGCCTGAGTTCGATC
GCAGTAAGTTCCCCGAATTGATGGAACAACTCGATGATGGTATTTGGCAGGATAATAGTGCCAACGAGCAGGCGAAAGCG
CGTACTGAATCTGCGCTTAGTCGCTAA

Upstream 100 bases:

>100_bases
TATTCTTACCTTTATTTGGAAGGATTCATTCACCACAAATGTTGCACCTCGCTAAGTATAGGTGGAGGGAGGCATTTGCT
ACGGGAAGAAGTAAAATAAT

Downstream 100 bases:

>100_bases
ACTCAAGTTCACTGATTAGCGCTATCGGAAAAACCACGTCATCCGTACGTGGTTTTTTTATGCTTGTTCAGTATCATAGT
GGCGTATTTTTTTGTTGGAG

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTLRLVFIQLTHFKHSLKVMTMSRQGCESGLSSFQVFCTSMAARVGAGNMAG
VAVAIGAAGPGAVFWMWLIAMLGMATAMVESTLAQVYKVRDTNGQFRGGPSYYMEKGLGQRWMGVLFAFFLIIAFGLVFN
AVQANTITGAMERVFGFNPTYVGIGLVLASGFVIVGGLRKVARVSEFIVPIMALAYILIAFIIVLFNLDQLPAIISLIVK
SAFGWQEAAAGGVAYTVAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAIIL
LSGDIGSSEDGIRLTINAMSNHVGDWGGAFIAIAIFLFCFTSIIANYSYAETNVMFLTGNSTKALPLFRLCVLGMVMFGA
VAKISLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYCEQLKSGKMPEFDRSKFPELMEQLDDGIWQDNSANEQAKA
RTESALSR

Sequences:

>Translated_488_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTLRLVFIQLTHFKHSLKVMTMSRQGCESGLSSFQVFCTSMAARVGAGNMAG
VAVAIGAAGPGAVFWMWLIAMLGMATAMVESTLAQVYKVRDTNGQFRGGPSYYMEKGLGQRWMGVLFAFFLIIAFGLVFN
AVQANTITGAMERVFGFNPTYVGIGLVLASGFVIVGGLRKVARVSEFIVPIMALAYILIAFIIVLFNLDQLPAIISLIVK
SAFGWQEAAAGGVAYTVAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAIIL
LSGDIGSSEDGIRLTINAMSNHVGDWGGAFIAIAIFLFCFTSIIANYSYAETNVMFLTGNSTKALPLFRLCVLGMVMFGA
VAKISLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYCEQLKSGKMPEFDRSKFPELMEQLDDGIWQDNSANEQAKA
RTESALSR
>Mature_488_residues
MLETIVNFLNALLWGKLLVYGLVGAGLYFTLRLVFIQLTHFKHSLKVMTMSRQGCESGLSSFQVFCTSMAARVGAGNMAG
VAVAIGAAGPGAVFWMWLIAMLGMATAMVESTLAQVYKVRDTNGQFRGGPSYYMEKGLGQRWMGVLFAFFLIIAFGLVFN
AVQANTITGAMERVFGFNPTYVGIGLVLASGFVIVGGLRKVARVSEFIVPIMALAYILIAFIIVLFNLDQLPAIISLIVK
SAFGWQEAAAGGVAYTVAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAIIL
LSGDIGSSEDGIRLTINAMSNHVGDWGGAFIAIAIFLFCFTSIIANYSYAETNVMFLTGNSTKALPLFRLCVLGMVMFGA
VAKISLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYCEQLKSGKMPEFDRSKFPELMEQLDDGIWQDNSANEQAKA
RTESALSR

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=470, Percent_Identity=48.5106382978723, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52164; Mature: 52164

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCC
>Mature Secondary Structure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HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]