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The map label for this gene is dnaG [H]

Identifier: 117921524

GI number: 117921524

Start: 3697709

End: 3699433

Strand: Direct

Name: dnaG [H]

Synonym: Shewana3_3086

Alternate gene names: 117921524

Gene position: 3697709-3699433 (Clockwise)

Preceding gene: 117921523

Following gene: 117921525

Centisome position: 74.37

GC content: 47.25

Gene sequence:

>1725_bases
ATGGCAATACCTCGTGATTTTATCAATGAGCTAATCGCTCGCACCGACATTGTCGAGCTTATCGACCGCAAGGTGCCCTT
GAAAAAGGCGGGTAAAAACTACTCGGCCTGTTGTCCTTTTCATAGCGAAAAATCACCTTCGTTTACCGTTAGCCGCGATA
AACAGTTTTATCATTGTTTTGGCTGCGGTGCCCACGGCAACGCCATCGATTTTGTGATGGAGTACGACAGACTCGACTTT
GTCGATGCCATAGAAGATCTTGCCGGACAACTGGGCTTAGAAGTCCCAAGGGAACAAGGCACGGGTAAACGCCAAGACGA
TGGCCTAAGCCGTGATCTATACCAATTAATGGAAGAAGCGAGCCGCTTTTTTCAAACTCAGTTAAGACAACACCAAGACA
AGCAAAAAGTCATCGACTACCTCGCCTATCGCGGATTATCCGATGATATTGTTGAGCATTTTGGGATTGGTTTTGCGCCC
GACGGTTGGGATGGCTTATTAAGCCGCTACCGACACAGTCAAGATGCACAGGATAAACTCCTGACCGCAGGCATGCTGAT
CAGCAATGACAGCGGTAAAAGATACGACAGATTCCGTGACCGCCTGATGTTTCCTATTCGCGACCGCCGTGGTCGTGTGA
TTGGATTTGGTGGCAGAGTATTGGGAGATGGCACCCCCAAGTACTTGAATTCGCCAGAAACGCCCATATTTCATAAGGGC
AATGAACTCTACGGCCTATACGAGCTAAAACAACGTCATCGCGATCCCGATAAGGTACTGATTGTCGAAGGCTATATGGA
CGTGGTCGCCCTCGCCCAATATGGCGTGGATTACGCGGTCGCCTCCCTTGGCACATCGACGACGGCTGAACAATTCCAAT
TGTTGCTGCGTAGTGCTAAGGAAGTGATTTGTTGTTATGACGGCGATAGAGCCGGTAATGAGGCCGCTTGGCGCGCCTTA
GAAACTGCTTTACCCCTACTCAAACCAGGGGACAAAGTGCGTTTTATGTTTTTACCTCAATCTGAAGATCCGGATTCAAT
GGTTCGTAAGATTGGTAAAGACGCATTCGAGCAAATGATGGAAAGTGCCATCACCCTGCCCGAATTTTTGTTTGATACAT
TAGCGACAAAATTTGGTACCGATAAAGGTAACTTAGCCAAACAAGCCTTTGGCCTAATTGAAAAAATTCAAGATACTGTG
CTGCAAAACCTACTGCTTGAGAACTTAGCTCACAAGTTAGGCATGAACAGCTCAGACGATATGAAGAAAAAACTGGGTTT
TAGTGTCAAACAAACTAAGCCCACAGCATCAACGGGACTCAAAGGACGCGGTACACCATTGCGACTTGCCATCGCCTTGT
TAGTACAGCATCCCGAGCTGGGAGTGGGATTATCCCCACAACCGGCATTAAACCATCTGCAAATGCCAGGCATCGACTTG
CTGCCATTGCTGTTGGAATTAACACGAGAGCATAGGTTAAACAGCGCACAACTACTTGAGCAATTCAGAGACAGCCCCCA
CAGCGGGACGCTGCATAAATTAGCCCAATGGGACCATCAAGTGGCGGATGAAAACCTGCTCGGAGAGTTTAAAAAAGCCC
TGATTTGGCTGAACAATCAATATATTGAGCAGCGATATCAGGAATTGAGTCTTAAACAGACCCATACTAAAGAAGAATGG
ATACAGCTGCAGAAGCTGATCTTAGTCATGAAAGGACTAAACTGA

Upstream 100 bases:

>100_bases
GTGCAAAATTGCAATAATTAGCGTTTCAATGTATGAACAAGCCGTGCCTATGCGCGGCTTGTTTGTTTAAATTCGCGCAA
GGCGAGATTTTAAGCACTCA

Downstream 100 bases:

>100_bases
GGCAAAAATGTCCTCAATCAGTATCCCAGTGACTAGCACAGGTAAGTGCACATGGCTATAATTGACGATTTGCGCGGCAC
TTAGCACAAGCTAACTGTCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
VDAIEDLAGQLGLEVPREQGTGKRQDDGLSRDLYQLMEEASRFFQTQLRQHQDKQKVIDYLAYRGLSDDIVEHFGIGFAP
DGWDGLLSRYRHSQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHRDPDKVLIVEGYMDVVALAQYGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGNEAAWRAL
ETALPLLKPGDKVRFMFLPQSEDPDSMVRKIGKDAFEQMMESAITLPEFLFDTLATKFGTDKGNLAKQAFGLIEKIQDTV
LQNLLLENLAHKLGMNSSDDMKKKLGFSVKQTKPTASTGLKGRGTPLRLAIALLVQHPELGVGLSPQPALNHLQMPGIDL
LPLLLELTREHRLNSAQLLEQFRDSPHSGTLHKLAQWDHQVADENLLGEFKKALIWLNNQYIEQRYQELSLKQTHTKEEW
IQLQKLILVMKGLN

Sequences:

>Translated_574_residues
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDF
VDAIEDLAGQLGLEVPREQGTGKRQDDGLSRDLYQLMEEASRFFQTQLRQHQDKQKVIDYLAYRGLSDDIVEHFGIGFAP
DGWDGLLSRYRHSQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
NELYGLYELKQRHRDPDKVLIVEGYMDVVALAQYGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGNEAAWRAL
ETALPLLKPGDKVRFMFLPQSEDPDSMVRKIGKDAFEQMMESAITLPEFLFDTLATKFGTDKGNLAKQAFGLIEKIQDTV
LQNLLLENLAHKLGMNSSDDMKKKLGFSVKQTKPTASTGLKGRGTPLRLAIALLVQHPELGVGLSPQPALNHLQMPGIDL
LPLLLELTREHRLNSAQLLEQFRDSPHSGTLHKLAQWDHQVADENLLGEFKKALIWLNNQYIEQRYQELSLKQTHTKEEW
IQLQKLILVMKGLN
>Mature_573_residues
AIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCFGCGAHGNAIDFVMEYDRLDFV
DAIEDLAGQLGLEVPREQGTGKRQDDGLSRDLYQLMEEASRFFQTQLRQHQDKQKVIDYLAYRGLSDDIVEHFGIGFAPD
GWDGLLSRYRHSQDAQDKLLTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKGN
ELYGLYELKQRHRDPDKVLIVEGYMDVVALAQYGVDYAVASLGTSTTAEQFQLLLRSAKEVICCYDGDRAGNEAAWRALE
TALPLLKPGDKVRFMFLPQSEDPDSMVRKIGKDAFEQMMESAITLPEFLFDTLATKFGTDKGNLAKQAFGLIEKIQDTVL
QNLLLENLAHKLGMNSSDDMKKKLGFSVKQTKPTASTGLKGRGTPLRLAIALLVQHPELGVGLSPQPALNHLQMPGIDLL
PLLLELTREHRLNSAQLLEQFRDSPHSGTLHKLAQWDHQVADENLLGEFKKALIWLNNQYIEQRYQELSLKQTHTKEEWI
QLQKLILVMKGLN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=574, Percent_Identity=50.3484320557491, Blast_Score=556, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65013; Mature: 64882

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFVDAIEDLAGQLGLEVPREQGTGKRQDDGLSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHH
SRFFQTQLRQHQDKQKVIDYLAYRGLSDDIVEHFGIGFAPDGWDGLLSRYRHSQDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHH
LTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHHHEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCHHHCCCCCCCCEECC
NELYGLYELKQRHRDPDKVLIVEGYMDVVALAQYGVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDRAGNEAAWRALETALPLLKPGDKVRFMFLPQSEDPDSMVRKIGKDAFEQMM
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ESAITLPEFLFDTLATKFGTDKGNLAKQAFGLIEKIQDTVLQNLLLENLAHKLGMNSSDD
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MKKKLGFSVKQTKPTASTGLKGRGTPLRLAIALLVQHPELGVGLSPQPALNHLQMPGIDL
HHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHH
LPLLLELTREHRLNSAQLLEQFRDSPHSGTLHKLAQWDHQVADENLLGEFKKALIWLNNQ
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIEQRYQELSLKQTHTKEEWIQLQKLILVMKGLN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPRDFINELIARTDIVELIDRKVPLKKAGKNYSACCPFHSEKSPSFTVSRDKQFYHCF
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEEECCCCEEEEE
GCGAHGNAIDFVMEYDRLDFVDAIEDLAGQLGLEVPREQGTGKRQDDGLSRDLYQLMEEA
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHH
SRFFQTQLRQHQDKQKVIDYLAYRGLSDDIVEHFGIGFAPDGWDGLLSRYRHSQDAQDKL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHH
LTAGMLISNDSGKRYDRFRDRLMFPIRDRRGRVIGFGGRVLGDGTPKYLNSPETPIFHKG
HHHHHEEECCCCHHHHHHHHHCCCCCCCCCCCEEEECCEEECCCCHHHCCCCCCCCEECC
NELYGLYELKQRHRDPDKVLIVEGYMDVVALAQYGVDYAVASLGTSTTAEQFQLLLRSAK
CCEEHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHH
EVICCYDGDRAGNEAAWRALETALPLLKPGDKVRFMFLPQSEDPDSMVRKIGKDAFEQMM
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ESAITLPEFLFDTLATKFGTDKGNLAKQAFGLIEKIQDTVLQNLLLENLAHKLGMNSSDD
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MKKKLGFSVKQTKPTASTGLKGRGTPLRLAIALLVQHPELGVGLSPQPALNHLQMPGIDL
HHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCHHH
LPLLLELTREHRLNSAQLLEQFRDSPHSGTLHKLAQWDHQVADENLLGEFKKALIWLNNQ
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIEQRYQELSLKQTHTKEEWIQLQKLILVMKGLN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]